不同产羔数绵羊性腺轴比较转录组研究

发布时间：2018-10-17 13:44

【摘要】：试验通过对巴美肉羊性腺轴进行转录组分析,旨在挖掘影响多羔性状的关键功能基因。分别选取双羔组巴美肉羊5只、单羔组4只,利用转录组测序(RNA-Seq)技术对下丘脑、垂体、卵巢转录组文库进行测序,然后对得到的测序数据进行生物信息学分析。测序数据经过质量控制后,27个样品共得到112Gb的有效数据。差异基因分析表明,与单羔组相比,双羔组下丘脑中,上调表达基因111个,下调表达基因106个;垂体组织中,上调表达基因97个,下调表达基因142个;卵巢组织中,上调表达基因182个,下调表达基因67个。功能富集性分析表明,神经活性的配体-受体相互作用信号通路在下丘脑-垂体-卵巢性腺轴调控排卵及卵泡发育方面发挥着重要的调控作用。通过不同产羔数巴美肉羊性腺轴比较转录组研究,提供了全部的转录本信息,筛选了影响产羔数的相关功能基因,丰富和补充了绵羊基因组信息,为进一步阐明绵羊繁殖力差异的分子机制奠定基础。
[Abstract]:By transcriptome analysis of gonad axis in Bamei sheep, the key functional genes affecting the traits of lambs were excavated. Five lambs and four lambs were selected respectively. The hypothalamus, pituitary and ovary transcriptional libraries were sequenced by transcriptional sequencing (RNA-Seq) technique, and the sequence data were analyzed by bioinformatics. After quality control, 27 samples obtained valid 112Gb data. Differential gene analysis showed that, in the hypothalamus, 111 up-regulated genes and 106 down-regulated genes were found in the hypothalamus, 97 up-regulated genes and 142 down-regulated genes in pituitary tissues. There were 182 up-regulated genes and 67 down-regulated genes. Functional enrichment analysis showed that the ligand-receptor interaction signaling pathway of nerve activity plays an important role in the regulation of ovulation and follicular development by hypothalamus-pituitary-ovarian gonadal axis. Based on the comparative transcriptome study of gonad axis in different lambs, the complete transcription information was provided, and the related functional genes affecting lambing size were screened, which enriched and supplemented the genomic information of sheep. It lays a foundation for further elucidating the molecular mechanism of sheep fecundity difference.
【作者单位】：内蒙古农牧业科学院;内蒙古农业大学动物科学学院;内蒙古ATCG生物信息研究所;巴彦淖尔市家畜改良工作站;中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与种质创新重点实验室;
【基金】：国家自然科学基金项目(31360542) 国家肉羊产业技术体系建设项目(CARS-39) 内蒙古自治区农牧业科学院创新基金(2015CXJJM02) 内蒙古自治区自然科学基金博士基金(2016BS(LH)0303) 中国农业科学院科技创新工程(ASTIP-IAS13)
【分类号】：S826

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