杜仲叶影响绵羊血清代谢组学的研究

发布时间：2018-10-17 21:17

【摘要】：为探索杜仲叶(Eucommia ulmoides leaves,EUL)对绵羊营养代谢和生理功能的影响,本研究选取25~30kg、70~80日龄的湖羊30只,随机分为3组,每组10只,分别为对照组(无杜仲叶日粮组,CTL)、低剂量添加组(10%杜仲叶日粮组,EUL1)、高剂量添加组(20%杜仲叶日粮组,EUL2)。预试期15d,正试期90d。试验结束时,通过静脉采血,离心后分别取血浆和血清,血浆用于生化分析,血清用于代谢组学分析。结果显示,饲喂杜仲叶后,EUL1和EUL2组绵羊血浆中葡萄糖、非酯化脂肪酸、高密度脂蛋白和极低密度脂蛋白均升高,EUL2组与CTL组差异显著或极显著(P0.05;P0.01),EUL1组与CTL组差异不显著(P0.05);EUL1和EUL2组尿素较CTL组极显著降低(P0.01),总胆固醇水平显著降低(P0.05),EUL1和EUL2组间差异不显著(P0.05)。血清样品采用液相色谱—质谱联用仪(LC-MS)进行检测,在正模式下得到593个代谢物,在负模式下得到1 570个代谢物。通过主成分分析(PCA)、偏最小二乘判别分析(PLS-DA)及正交偏最小二乘判别分析(OPLS-DA)发现,各试验组间的差异代谢物较多,能将其得分图明显区分。进一步对变量重要性因子大于1.0(VIP1.0)的代谢物进行t检验后发现,各组之间有24种特征代谢物差异显著(P0.05)。这些差异代谢物涉及体内葡萄糖、脂肪酸和氨基酸等的代谢,有的代谢物还与动物的免疫机能相关。杜仲叶饲料对绵羊的营养代谢、生理状态和免疫状况可产生明显的影响,代谢组学可有针对性地分析代谢物发生的步骤及生理作用,为阐明杜仲叶影响营养代谢的机制提供参考。
[Abstract]:In order to explore the effect of Eucommia ulmoides leaf (Eucommia ulmoides leaves,EUL on the nutritional metabolism and physiological function of sheep, 30 Hu sheep (2530 kg / kg) were randomly divided into 3 groups (10 in each group). The rats were divided into two groups: control group (low dose, CTL), diet without Eucommia ulmoides leaf diet group, 10% Eucommia ulmoides leaf diet group, EUL1) and high dose supplementation group (20% Eucommia ulmoides leaf diet group, EUL2 group). The pre-test period was 15 days and the positive test period was 90 days. At the end of the experiment, blood was collected through vein, and plasma and serum were collected after centrifugation. Plasma was used for biochemical analysis and serum was used for metabolomics analysis. The results showed that after feeding Eucommia ulmoides leaves, the plasma glucose and non-esterified fatty acids in EUL1 and EUL2 groups were determined. High density lipoprotein and very low density lipoprotein increased, the difference between EUL2 group and CTL group was significant or extremely significant (P0.05, P0.01), the difference between EUL1 group and CTL group was not significant (P0.05), the urea in EUL1 and EUL2 group was significantly lower than that in CTL group (P0.01), the total cholesterol level was significantly lower (P0.05), and the difference between EUL1 and EUL2 group was not significant (P0.05). Serum samples were detected by liquid chromatography-mass spectrometry (LC-MS). 593 metabolites were obtained in positive mode and 1 570 metabolites in negative mode. By principal component analysis (PCA),) partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA), it was found that there were more different metabolites among the test groups, and their score diagrams could be distinguished obviously. Further t test of metabolites with variable importance factor greater than 1. 0 (VIP1.0) showed that there were significant differences in 24 characteristic metabolites among the groups (P0.05). These metabolites are related to the metabolism of glucose, fatty acids and amino acids, and some metabolites are related to the immune function of animals. The leaf feed of Eucommia ulmoides could have obvious effects on the nutritional metabolism, physiological state and immune status of sheep, and metabolomics could be used to analyze the steps and physiological effects of metabolites. In order to elucidate the mechanism of Eucommia ulmoides leaves affecting nutrient metabolism.
【作者单位】：河南农业大学牧医工程学院农业部动物生化与营养重点试验室;河南农业大学现代试验技术与管理中心;洛阳市龙须坡农牧有限公司;河南省汝阳县农牧局;平顶山市畜牧局;济源市动物卫生监督所;郑州市现代农业科技服务中心;
【基金】：河南省科技厅科技惠民计划项目:信息化技术在杜仲羊生产中的应用(162207110009)
【分类号】：S826.5

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