贵州省安顺地区蛇类裂头蚴感染调查及生物学特性的研究
发布时间：2018-10-17 22:00
【摘要】：目的了解贵州省安顺地区蛇类自然感染猬裂头蚴的情况,并对蛇源裂头蚴的生物学特性进行研究。方法①将野生蛇处死,去皮后按顺序编号。检测蛇体内猬裂头蚴的感染情况,观察蛇体感染裂头蚴后的病理组织变化;②口服法以5条/只裂头蚴感染昆明小鼠,于感染后10min、20min、30min、1h、2h、4h、6h、24h、7d、14d分别处死一组小鼠(3只小鼠/组),观察裂头蚴在小鼠体内的移行及分布;③将游离的或有囊包的裂头蚴分别感染二组小鼠(每组6只,每只感染5条裂头蚴),记录二组小鼠的感染率;④将80只小鼠,分成8组(每组10只,每只感染3条裂头蚴)。1~4组小鼠分别经口感染,在100%蛇胆汁浓度下处理0.5h、3h、6h或12h的裂头蚴,5~8组为生理盐水处理0.5h、3h、6h或12h的对照组;⑤将20只小鼠,分成2组,每组10只。第1组每只感染5条蛇株裂头蚴,第2组每只感染5条蛙株裂头蚴。2周后剖杀小鼠,收集小鼠体内的裂头蚴,计数各组裂头蚴的检出率。结果①在捕捉到的50条野生蛇中裂头蚴的感染率为78.0%(39/50),阳性蛇的感染强度为2~347条;在蛇体内,裂头蚴主要寄生于皮下(45.2%),其次为体腔(39.8%)及肌肉(15.0%);②发现小鼠经口服感染裂头蚴后,裂头蚴最早于10 min穿透胃肠壁;感染后20 min,腹腔内已见穿过肠壁的裂头蚴;感染后10 min至6 h,裂头蚴主要见于胃肠腔、胃肠壁和腹腔内;感染24 h,有少数的裂头蚴移行至小鼠皮下;感染7 d后,大多数的裂头蚴移行至皮下组织,从颈部、躯干及头部皮下组织检获的裂头蚴数分别为7、6和1条;脑、心脏、肝脏、肾脏及肺等器官未见裂头蚴寄生;③蛇体内的裂头蚴有三种致病状态,分别为游离状态、薄壁囊包的虫体及变性坏死囊包虫体。游离虫体对周围肌肉组织有轻度的挤压,见少量的嗜中性粒细胞浸润。薄壁囊包的虫体,其囊壁薄,由一层纤维组织形成,内含少量的炎性细胞,囊内虫体活动好。变性坏死囊包虫体,囊内的虫体逐渐由坏死无组织结构的虫体残片及嗜酸性物质替代,囊壁增厚,壁内的炎性细胞增多,主要以淋巴细胞为主,且囊壁出现早期的肉芽肿反应。用游离状态和有囊包壁囊包的裂头蚴感染小鼠后,两组的感染率分别为43.33%和56.67%,两组之间无明显差异(P0.05);④经胆汁浸泡后的裂头蚴对小鼠的感染性与生理盐水对照组比较,两者无明显差异(P0.05);⑤与蛙株裂头蚴相比,蛇株裂头蚴对小鼠的感染性无明显差异(P0.05)。结论贵州安顺地区野生蛇类裂头蚴感染严重,存在食品安全隐患。蛇类裂头蚴感染率的调查及生物学特性的研究有助于裂头蚴病致病机制、诊断及预防等方面的后续研究。
[Abstract]:Objective to investigate the natural infection of serpentine mitomycaria in Anshun, Guizhou Province, and to study the biological characteristics of serpentine mitomycaria. Methods 1 the wild snake was killed and the skin was removed and numbered in sequence. To detect the infection of mitomycaria in snake body and to observe the pathological changes of snake body after infection with mitomycaria. Ten minutes after infection, a group of mice (3 mice / group) were killed to observe the migration and distribution of mitomycaria in vivo, and the free or encapsulated mitomycariae were infected with two groups of mice (6 mice in each group, respectively) at 10 min, 20 min, 30 min, 1 h, 2 h, 4 h, 24 h, 7 d, 14 days, respectively. (4) 80 mice were divided into 8 groups (10 in each group and 3 in each). At the concentration of 100% snake bile, the mitomycaria was treated at 0.5 h, 3h, 6h or 12h, and the control group was treated with normal saline for 0.5h, 3h, 6h or 12h, and (5) 20 mice were divided into 2 groups, 10 rats in each group. The first group was infected with 5 filariae per snake strain, and the second group was infected with 5 frog strain mitomycaria. After 2 weeks, mice were dissected to collect the mitocercaria in mice, and the positive rate of each group was counted. Results (1) the infection rate of mitomycaria was 78.0% (39 / 50), and the infection intensity of positive serpent was 21,347. In the snake, the caterpillar was mainly parasitic on the skin (45.2%), followed by the body cavity (39.8%) and muscle (15.0%). Mitomycaria first penetrated the gastrointestinal wall at 10 min, and 20 min, after infection, the mitomycaria was found in the abdominal cavity, stomach wall and abdominal cavity from 10 min to 6 h after infection, and was mainly found in the gastrointestinal cavity, gastroenteric wall and abdominal cavity at 10 min to 6 h after infection. 7 days after infection, most of the mitomycaria migrated to subcutaneous tissue, and the number of mitomycaria seized from neck, trunk and head subcutaneous tissue was 76 and 1 respectively, brain, heart, liver, brain, heart and liver, brain, heart, liver, brain, heart and liver respectively. There were three pathogenetic states in the hydatid of the snake, which were free, thin wall cyst and denatured necrotic hydatid. The free body slightly compresses the surrounding muscle tissue and shows a small amount of neutrophil infiltration. Thin-walled cyst with a thin wall formed by a layer of fibrous tissue containing a small number of inflammatory cells. Denatured necrotic cyst hydatid, the cyst body was gradually replaced by necrotic and acidophilic substance, the cyst wall thickened, the inflammatory cells in the wall increased, mainly lymphocytes, and the early granuloma reaction occurred in the cyst wall. The infection rates of the two groups were 43.33% and 56.67%, respectively, and there was no significant difference between the two groups (P0.05). There was no significant difference between the two groups (P0.05), and 5 there was no significant difference in the infectivity of serpentine caterpillar to mice compared with frog strain (P0.05). Conclusion the infection of wild serpentine mitomycaria in Anshun area of Guizhou Province is serious and there are hidden dangers of food safety. The investigation of infection rate and biological characteristics of mitomycaria of snake is helpful to further study on pathogenesis, diagnosis and prevention of mitomycosis.
【学位授予单位】：贵阳医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.9
本文编号：2278137
[Abstract]:Objective to investigate the natural infection of serpentine mitomycaria in Anshun, Guizhou Province, and to study the biological characteristics of serpentine mitomycaria. Methods 1 the wild snake was killed and the skin was removed and numbered in sequence. To detect the infection of mitomycaria in snake body and to observe the pathological changes of snake body after infection with mitomycaria. Ten minutes after infection, a group of mice (3 mice / group) were killed to observe the migration and distribution of mitomycaria in vivo, and the free or encapsulated mitomycariae were infected with two groups of mice (6 mice in each group, respectively) at 10 min, 20 min, 30 min, 1 h, 2 h, 4 h, 24 h, 7 d, 14 days, respectively. (4) 80 mice were divided into 8 groups (10 in each group and 3 in each). At the concentration of 100% snake bile, the mitomycaria was treated at 0.5 h, 3h, 6h or 12h, and the control group was treated with normal saline for 0.5h, 3h, 6h or 12h, and (5) 20 mice were divided into 2 groups, 10 rats in each group. The first group was infected with 5 filariae per snake strain, and the second group was infected with 5 frog strain mitomycaria. After 2 weeks, mice were dissected to collect the mitocercaria in mice, and the positive rate of each group was counted. Results (1) the infection rate of mitomycaria was 78.0% (39 / 50), and the infection intensity of positive serpent was 21,347. In the snake, the caterpillar was mainly parasitic on the skin (45.2%), followed by the body cavity (39.8%) and muscle (15.0%). Mitomycaria first penetrated the gastrointestinal wall at 10 min, and 20 min, after infection, the mitomycaria was found in the abdominal cavity, stomach wall and abdominal cavity from 10 min to 6 h after infection, and was mainly found in the gastrointestinal cavity, gastroenteric wall and abdominal cavity at 10 min to 6 h after infection. 7 days after infection, most of the mitomycaria migrated to subcutaneous tissue, and the number of mitomycaria seized from neck, trunk and head subcutaneous tissue was 76 and 1 respectively, brain, heart, liver, brain, heart and liver, brain, heart, liver, brain, heart and liver respectively. There were three pathogenetic states in the hydatid of the snake, which were free, thin wall cyst and denatured necrotic hydatid. The free body slightly compresses the surrounding muscle tissue and shows a small amount of neutrophil infiltration. Thin-walled cyst with a thin wall formed by a layer of fibrous tissue containing a small number of inflammatory cells. Denatured necrotic cyst hydatid, the cyst body was gradually replaced by necrotic and acidophilic substance, the cyst wall thickened, the inflammatory cells in the wall increased, mainly lymphocytes, and the early granuloma reaction occurred in the cyst wall. The infection rates of the two groups were 43.33% and 56.67%, respectively, and there was no significant difference between the two groups (P0.05). There was no significant difference between the two groups (P0.05), and 5 there was no significant difference in the infectivity of serpentine caterpillar to mice compared with frog strain (P0.05). Conclusion the infection of wild serpentine mitomycaria in Anshun area of Guizhou Province is serious and there are hidden dangers of food safety. The investigation of infection rate and biological characteristics of mitomycaria of snake is helpful to further study on pathogenesis, diagnosis and prevention of mitomycosis.
【学位授予单位】：贵阳医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.9
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