益生菌对柔嫩艾美尔球虫感染雏鸡免疫水平的影响及免疫保护效果评价
发布时间：2018-10-18 13:14
【摘要】：鸡球虫病呈全球性分布,在养禽业中普遍存在且危害极大,往往造成巨大的经济损失且严重阻碍养禽业发展。益生菌有望成为防治球虫病的一条新路径。由于益生菌的指代范围极其广泛,所以本研究以乳酸菌为例探究益生菌对柔嫩艾美尔球虫(E.tenella)感染雏鸡免疫水平的影响及其免疫保护效果。本研究以1~31日龄雄性肉雏鸡为实验动物,并选取了3种乳酸菌菌株进行试验,试验选用的3种乳酸菌菌株分别为鼠李糖乳杆菌(Lactobacillus rhamnosus)ATCC53103、乳酸片球菌(Pediococcus acidilactici)ATCC8042和植物乳杆菌(Lactobacillus plantarum)WCFS1。将体重相近,健康的250只1日龄肉用雏鸡随机分为A、C、D、E 5组,每组50只分别饲养于经严格消毒且无球虫感染的环境中。雏鸡1~21日龄期间,A、B两组每日饲喂前空腹灌服生理盐水200μL/只,C、D、E三组每日饲喂前空腹灌服各自相应菌种2×108cfu/200μL,其中C组雏鸡灌服Lactobacillus rhamnosus ATCC53103,D组雏鸡灌服Pediococcus acidilactici ATCC8042,E组雏鸡灌服Lactobacillus plantarum WCFS1。雏鸡22日龄时,每组处死25只进行外周血中淋巴细胞增殖能力的检测、CD3+CD4+和CD3+CD8+T淋巴细胞含量的检测、细胞因子IL-2和IFN-γ分泌量的检测、抗体Ig G含量的检测以及食糜中SIgA含量的检测。除A组外其它各组余下雏鸡分别经口感染已孢子化的E.tenella卵囊5×104个/只,攻虫8天后,雏鸡31日龄时处死全部雏鸡,除检测上述免疫指标外,对雏鸡的相对增重率、血便记分、盲肠病变程度、盲肠内容物中的卵囊值、粪便中的卵囊排出量、抗球虫指数及病理组织切片也进行相应检测。具体试验结果如下:(1)乳酸菌对E.tenella感染雏鸡免疫水平的影响:球虫感染前、后,雏鸡22日龄和31日龄时分别对雏鸡进行相关免疫指标检测,结果表明试验所用三株乳酸菌在球虫感染前、后均能够提高雏鸡外周血中淋巴细胞的增殖能力,促进外周血中CD3+CD4+和CD3+CD8+T淋巴细胞的生成,进而提高外周血中细胞因子IL-2和IFN-γ的分泌量,而提高雏鸡细胞免疫水平;另外,试验所用三株乳酸菌也能够通过提高外周血中IgG抗体及肠内容物中SIgA抗体的分泌量从而提高雏鸡体液免疫水平。其中免疫保护作用效果最好的菌株是Lactobacillus rhamnosus ATCC53103,Lactobacillus plantarum WCFS1次之,Pediococcus acidilactici ATCC8042最次。(2)乳酸菌对鸡E.tenella的免疫保护效果评价:结果表明在球虫感染前、后,灌服乳酸菌的3组雏鸡其平均增重均比B组高,且C组和B相比差异显著(P㩳0.05);灌服乳酸菌的C、D、E三组雏鸡其血便记分均低于B组,且C组最低;B组雏鸡盲肠病变最严重,病变记分平均值达到3.25,极显著的高于C、E两组(P㩳0.01),显著的高于D组(P㩳0.05);灌服乳酸菌的C、D、E三组雏鸡其卵囊排出量均低于B组,且C组最低;灌服乳酸菌的三组雏鸡其抗球虫指数分别为152.40、123.22、133.26,均在120~160之间,具有中等的抗球虫效果,且Lactobacillus rhamnosusATCC53103的抗球虫效果最好,Lactobacillus plantarum WCFS1次之,Pediococcus acidilactici ATCC8042最次。
[Abstract]:Cocciasis is a global distribution, widespread and harmful in poultry industry, which often leads to huge economic losses and seriously hinders the development of poultry industry. Probiotics are expected to be a new path to the prevention and treatment of coccidia. In this study, lactic acid bacteria were used as an example to investigate the effect of probiotics on the immune level of chicken infected with Eimeria tenella (E.tenella) and its immune protective effect. Three lactic acid bacteria strains were selected as experimental animals and three kinds of lactic acid bacteria strains were selected. The three lactic acid bacteria strains selected were Lactobacillus rhamnosus ATCC 53103, coccus acidilactici ATCC 8042 and Lactobacillus plantarum WCFS1, respectively. The broiler chicks were randomly divided into A, C, D and E 5 groups, and 50 animals in each group were housed in an environment of strict disinfection and no coccidia infection. During the age of 1-21 days, two groups of groups A and B were fed with normal saline (200. mu.L/ L, C, D, E) before daily feeding, and the two groups were fed with the corresponding strain of 2 ~ 108cfu/ 200 & mu; L. Group E chicks were inoculated with Lactobacillus plantarum WCFS1. The detection of lymphocyte proliferation in peripheral blood, the detection of CD3 + CD4 + and CD3 + CD8 + T lymphocyte content, the detection of cytokine IL-2 and IFN-IL secretion, the detection of antibody Ig G content and the detection of SIgA content in chyme were carried out at the age of 22 days. Except for the above-mentioned immunological indexes, the relative weight gain, blood stool score and cecum lesion degree of chicks were determined. The egg sac value in the cecal contents, the amount of egg sac discharge in the feces, the anticoccidial index and the pathological tissue sections were also detected accordingly. The specific test results are as follows: (1) The effect of lactic acid bacteria on immune level of chickens infected with E.tenella is as follows: before and after the infection of coccidia, the chickens are tested with relevant immune indexes at the age of 22 days and the age of 31 days respectively, and the results show that the three lactic acid bacteria used in the test are before the infection of coccidian. The method can improve the proliferation ability of the lymphocytes in the peripheral blood of the chicken, promote the generation of CD3 + CD4 + and CD3 + CD8 + T lymphocytes in the peripheral blood, and further improve the secretion of the cytokine IL-2 and IFN-IL in the peripheral blood, thereby improving the immune level of the chicken cells; and additionally, The three lactic acid bacteria used in the test can also improve the humoral immunity level of the chicken by increasing the secretion amount of the SIgA antibody in the IgG antibody and the intestinal contents in the peripheral blood. Among them, the best strain was Lactobacillus rhamnosus ATCC 53103, Lactobacillus plantarum WCFS1 was the second, and gioctactici ATCC 8042 was the best. (2) The effect of lactic acid bacteria on the immune protection of chicken E. Lacella showed that the average weight gain was higher than that of group B before and after coccidia infection, and the difference between group C and B was significantly higher than that in group B (P? 0.05); C, D of lactic acid bacteria were perfused with lactic acid bacteria. The scores of blood stool were lower in group E than in group B, and the lowest in group C, and the average value of lesion score was 3. 25 in group B, which was significantly higher than that of group C and E (P? 0.01), which was significantly higher than that of group D (P? 0.05); C, D of lactic acid bacteria in group B were significantly higher than that in group D (P? 0.05). The number of egg sacs was lower in group E than in group B, and the lowest in C group. The anticoccidia index of the three groups with lactic acid bacteria was 152. 40, 123. 22, 133. 26, all of which were 120 ~ 160, had moderate anti-coccidia effect, and Lactiobacter rhamnosous 53103 had the best anti-coccidial effect, and Lactobacillus plantarum WCFS1 was the second.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.31
本文编号：2279232
[Abstract]:Cocciasis is a global distribution, widespread and harmful in poultry industry, which often leads to huge economic losses and seriously hinders the development of poultry industry. Probiotics are expected to be a new path to the prevention and treatment of coccidia. In this study, lactic acid bacteria were used as an example to investigate the effect of probiotics on the immune level of chicken infected with Eimeria tenella (E.tenella) and its immune protective effect. Three lactic acid bacteria strains were selected as experimental animals and three kinds of lactic acid bacteria strains were selected. The three lactic acid bacteria strains selected were Lactobacillus rhamnosus ATCC 53103, coccus acidilactici ATCC 8042 and Lactobacillus plantarum WCFS1, respectively. The broiler chicks were randomly divided into A, C, D and E 5 groups, and 50 animals in each group were housed in an environment of strict disinfection and no coccidia infection. During the age of 1-21 days, two groups of groups A and B were fed with normal saline (200. mu.L/ L, C, D, E) before daily feeding, and the two groups were fed with the corresponding strain of 2 ~ 108cfu/ 200 & mu; L. Group E chicks were inoculated with Lactobacillus plantarum WCFS1. The detection of lymphocyte proliferation in peripheral blood, the detection of CD3 + CD4 + and CD3 + CD8 + T lymphocyte content, the detection of cytokine IL-2 and IFN-IL secretion, the detection of antibody Ig G content and the detection of SIgA content in chyme were carried out at the age of 22 days. Except for the above-mentioned immunological indexes, the relative weight gain, blood stool score and cecum lesion degree of chicks were determined. The egg sac value in the cecal contents, the amount of egg sac discharge in the feces, the anticoccidial index and the pathological tissue sections were also detected accordingly. The specific test results are as follows: (1) The effect of lactic acid bacteria on immune level of chickens infected with E.tenella is as follows: before and after the infection of coccidia, the chickens are tested with relevant immune indexes at the age of 22 days and the age of 31 days respectively, and the results show that the three lactic acid bacteria used in the test are before the infection of coccidian. The method can improve the proliferation ability of the lymphocytes in the peripheral blood of the chicken, promote the generation of CD3 + CD4 + and CD3 + CD8 + T lymphocytes in the peripheral blood, and further improve the secretion of the cytokine IL-2 and IFN-IL in the peripheral blood, thereby improving the immune level of the chicken cells; and additionally, The three lactic acid bacteria used in the test can also improve the humoral immunity level of the chicken by increasing the secretion amount of the SIgA antibody in the IgG antibody and the intestinal contents in the peripheral blood. Among them, the best strain was Lactobacillus rhamnosus ATCC 53103, Lactobacillus plantarum WCFS1 was the second, and gioctactici ATCC 8042 was the best. (2) The effect of lactic acid bacteria on the immune protection of chicken E. Lacella showed that the average weight gain was higher than that of group B before and after coccidia infection, and the difference between group C and B was significantly higher than that in group B (P? 0.05); C, D of lactic acid bacteria were perfused with lactic acid bacteria. The scores of blood stool were lower in group E than in group B, and the lowest in group C, and the average value of lesion score was 3. 25 in group B, which was significantly higher than that of group C and E (P? 0.01), which was significantly higher than that of group D (P? 0.05); C, D of lactic acid bacteria in group B were significantly higher than that in group D (P? 0.05). The number of egg sacs was lower in group E than in group B, and the lowest in C group. The anticoccidia index of the three groups with lactic acid bacteria was 152. 40, 123. 22, 133. 26, all of which were 120 ~ 160, had moderate anti-coccidia effect, and Lactiobacter rhamnosous 53103 had the best anti-coccidial effect, and Lactobacillus plantarum WCFS1 was the second.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.31
【参考文献】
相关期刊论文 前2条
1 雷娜;陈书明;;鸡IFN-γ基因克隆表达及其抗球虫研究进展[J];中国畜牧兽医;2009年07期
2 张正黎;廖党金;;球虫疫苗研究进展[J];中国兽医寄生虫病;2008年04期
，本文编号：2279232
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