猪瘟兔化弱毒疫苗与猪圆环病毒2型灭活疫苗同时接种的免疫效果评价

发布时间：2018-10-18 16:35

【摘要】：为评价猪瘟兔化弱毒疫苗和猪圆环病毒2型(PCV2)灭活疫苗同时免疫效果,本实验采用猪瘟活疫苗(细胞源)和PCV2灭活疫苗(LG株)两种疫苗同时分点注射和混合注射35日龄仔猪,于免疫后间隔3周、5周、7周、9周采血,通过猪瘟病毒(CSFV)抗体检测试剂盒和PCV2抗体检测试剂盒进行CSFV和PCV2特异性抗体检测。结果显示,无论是两种疫苗同时颈部两侧分点分别注射,还是用PCV2灭活疫苗稀释CSFV活疫苗后混合免疫,免疫后3周CSFV和PCV2抗体均呈阳性,CSFV抗体滴度和PCV2抗体滴度于7周和9周达到峰值,与各疫苗单独免疫对照组抗体产生时间和滴度无明显差异。实验表明,两种疫苗可以同时注射或采用PCV2灭活疫苗稀释CSFV活疫苗进行免疫,均不影响各自免疫抗体的产生。因此,可以将PCV2灭活疫苗作为CSFV活疫苗的稀释剂,采用一次性注射的形式同时接种这两种疫苗,为简化这两种病临床疫苗免疫注射方面提供了实验依据。
[Abstract]:In order to evaluate the simultaneous immunization effect of hog fever attenuated rabbit vaccine and porcine circovirus type 2 (PCV2) inactivated vaccine, live vaccine (cell source) and PCV2 inactivated vaccine (LG strain) were used in this study. Blood samples were collected at intervals of 3 weeks, 5 weeks, 7 weeks and 9 weeks after immunization. CSFV and PCV2 specific antibodies were detected by (CSFV) antibody detection kit and PCV2 antibody detection kit. The results showed that either the two vaccines were injected separately on both sides of the neck at the same time, or the CSFV live vaccine was diluted with PCV2 inactivated vaccine. The antibody titers of CSFV and PCV2 were positive at 3 weeks after immunization. The titer of CSFV antibody and PCV2 antibody reached the peak at 7 weeks and 9 weeks after immunization. There was no significant difference between the antibody production time and titer of each vaccine alone compared with the control group. The results showed that the two vaccines could be injected simultaneously or diluted with PCV2 inactivated vaccine to immunize CSFV live vaccine without affecting the production of their respective immune antibodies. Therefore, the inactivated PCV2 vaccine can be used as the diluent of CSFV live vaccine, and the two vaccines can be inoculated simultaneously by one-off injection, which provides the experimental basis for simplifying the clinical immunization of the two diseases.
【作者单位】：中国农业大学生物学院;哈尔滨维科生物技术开发公司;
【分类号】：S858.28

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