山羊源益生芽胞杆菌的筛选

发布时间：2018-10-18 17:42

【摘要】：为获得山羊源优良益生芽胞杆菌菌株,利用刚果红染色法对26株山羊源芽胞杆菌进行产纤维素酶初筛,结合滤纸酶以及羧甲基纤维素(CMC)酶活力测定进行复筛,并对其耐酸、耐胆盐生物学特性进行研究,初步确定其益生特性,并进一步对经上述筛选出的菌株的抑菌性以及对药物的敏感性进行了测定。结果显示,从26株山羊源芽胞杆菌中初筛出4株产纤维素酶较好的芽胞杆菌,对4株芽胞杆菌产酶复筛以及对酸和胆盐的耐受性测定后,得到一株枯草芽胞杆菌,该菌株滤纸酶活为4.4U,CMC酶活为1610 U,在pH 3.0的环境中存活率大于50%,在0.3%的牛胆盐溶液中存活率高于70%,对金黄色葡萄球菌具有抑制作用,且对16种常用抗生素敏感。该株枯草芽胞杆菌X7F3可作为山羊源微生态制剂候选菌株。
[Abstract]:In order to obtain excellent strains of Bacillus probiotics from goat, 26 strains of Bacillus from goat were screened by Congo red staining method for cellulase production, combined with filter paper enzyme and carboxymethyl cellulose (CMC) enzyme activity. The biological characteristics of bile salt tolerance were studied, the prebiotic characteristics were preliminarily determined, and the bacteriostasis and drug sensitivity of the strains screened out above were determined. The results showed that four strains of Bacillus subtilis with good cellulase production were screened from 26 strains of Bacillus from goat, 4 strains of Bacillus subtilis were screened for enzyme production and the tolerance to acid and bile salt was determined, a strain of Bacillus subtilis was obtained. The filter paper enzyme activity of the strain was 1610 U, the survival rate in the environment of pH 3.0 was more than 50, and the survival rate in 0.3% of bovine bile salt solution was higher than 70%. The strain had inhibitory effect on Staphylococcus aureus and was sensitive to 16 common antibiotics. The strain of Bacillus subtilis X7F3 can be used as a candidate strain for goat microecological preparation.
【作者单位】：西南民族大学生命科学与技术学院;
【基金】：国家民委科技项目(14XNZ025) 四川省教改项目“兽医专业学位研究生教育实践基地建设”
【分类号】：S816

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