4种兽用注射液中非法添加倍他米松UPLC-PDA检查方法的建立
发布时间：2018-10-18 17:38
【摘要】：为了建立柴胡注射液、维生素C注射液、盐酸林可霉素注射液、黄芪多糖注射液中非法添加倍他米松的超高效液相色谱检查方法,采用Waters ACQUITY UPLC湟BEH C18色谱柱(2.1 mm×50 mm,1.7μm),以0.75%三乙胺溶液(用磷酸调节p H为3.0)-甲醇-乙腈(50∶45∶5)为流动相;流速为0.3 m L·min-1;二极管阵列检测器,波长采集范围为190~400 nm,记录色谱图波长242 nm。柱温25℃,进样量10μL。倍他米松质量浓度在20.0~180.0 mg·L-1内峰面积呈良好线性关系(R2=0.9997,n=7),在柴胡注射液、盐酸林可霉素注射液、维生素C注射液、黄芪多糖注射液中倍他米松的平均回收率分别为103.5%(RSD=0.2%)、101.2%(RSD=0.8%)、100.4%(RSD=1.7%)、99.2%(RSD=1.7%)。本方法简便、快速、准确,可用于柴胡注射液等四种兽用注射液中非法添加倍他米松的检测。
[Abstract]:In order to establish a method for the determination of betamethasone in Radix Bupleurum injection, Vitamin C injection, lincomycin Hydrochloride injection and Astragalus Polysaccharide injection by Ultra-high performance liquid Chromatography. Waters ACQUITY UPLC Huang BEH C18 column (2.1 mm 脳 50 mm,1.7 渭 m),) was used as mobile phase with 0.75% triethylamine solution (adjusted with phosphoric acid to pH 3.0) -methanol-acetonitrile (50:45:5), and the flow rate was 0.3 mL min-1; diode array detector. Wavelength acquisition range is 190,400 nm, recording chromatogram wavelength 242 nm. The column temperature is 25 鈩，
本文编号：2279887
[Abstract]:In order to establish a method for the determination of betamethasone in Radix Bupleurum injection, Vitamin C injection, lincomycin Hydrochloride injection and Astragalus Polysaccharide injection by Ultra-high performance liquid Chromatography. Waters ACQUITY UPLC Huang BEH C18 column (2.1 mm 脳 50 mm,1.7 渭 m),) was used as mobile phase with 0.75% triethylamine solution (adjusted with phosphoric acid to pH 3.0) -methanol-acetonitrile (50:45:5), and the flow rate was 0.3 mL min-1; diode array detector. Wavelength acquisition range is 190,400 nm, recording chromatogram wavelength 242 nm. The column temperature is 25 鈩，
本文编号：2279887
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