陕西省部分地区猪圆环病毒2型全基因组的遗传变异分析

发布时间：2018-10-19 12:31

【摘要】：为了解陕西省猪圆环病毒2型(PCV2)的遗传变异情况,根据GenBank登录的PCV2全基因组序列,设计1对引物,从陕西省部分地区规模化猪场疑似PCV2感染的病猪采集病料9份,应用PCR扩增PCV2的全基因,其中6份为阳性,并对扩增的6个PCV2全基因组序列进行测序和序列分析。基因测序表明,PCV2基因组全长为1 767bp;对6株病毒序列进行同源性比较,6个毒株全基因之间核苷酸同源性为98.3%~100%,与GenBank上已发表的国内外毒株全基因组比较,同源性为96.3%~97.8%;与近几年陕西株比较发现基因变异程度不稳定,与2013年分离株(KX352154.1)同源性最高,2015年分离株(KX352159.1)次之,反而与2014年分离株(KX068219.1)最低;对6个毒株的ORF1和ORF2基因进行同源性比较,6个毒株的ORF1和ORF2基因的核苷酸同源性分别为98.1%~100%和98.2%~100%,与GenBank上已发表的国内外参考株ORF1和ORF2基因进行同源性比较,同源性分别为97.6%~99.8%和91.5~96.0%。陕西省流行的PCV2基因组较为保守,变异不大,同源性很高。
[Abstract]:In order to understand the genetic variation of porcine circovirus type 2 (PCV2) in Shaanxi Province, a pair of primers was designed according to the whole PCV2 genome sequence registered by GenBank, and 9 samples were collected from suspected PCV2 infected pigs in some parts of Shaanxi Province. The whole gene of PCV2 was amplified by PCR, 6 of them were positive, and the whole genomic sequences of 6 PCV2 were sequenced and sequenced. Gene sequencing showed that the total length of PCV2 genome was 1767 BP, and the nucleotide homology among the whole genes of 6 strains was 98.3100, which was compared with the whole genome of domestic and foreign strains published on GenBank. Compared with Shaanxi strain in recent years, the degree of gene variation was unstable, the homology was the highest with KX352154.1 in 2013, followed by KX352159.1 in 2015 and the lowest in 2014 (KX068219.1). The nucleotide homology of ORF1 and ORF2 genes in 6 strains was 98.1100.The homology was 97.6% and 91.596.0%, respectively, compared with ORF1 and ORF2 genes, which had been published at home and abroad on GenBank, and the nucleotide homology of ORF1 gene and ORF2 gene of 6 strains were 98.1% and 98.2100%, respectively, and the homology was 91.596% and 91.596%, respectively, compared with ORF1 and ORF2 genes published at home and abroad on GenBank. The prevalent PCV2 genome in Shaanxi Province is more conserved, with little variation and high homology.
【作者单位】：西北农林科技大学动物医学院;
【基金】：陕西省农业科技创新与攻关项目(2016NY-095) 西北农林科技大学基本科研业务费项目(Z109021427)
【分类号】：S852.651

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