一株鸭源H6N6亚型禽流感病毒的基因组测序及遗传进化分析

发布时间：2018-10-20 08:19

【摘要】：对2016年分离自华东地区某活禽交易市场的一株鸭源H6N6亚型禽流感病毒A/duck/Anhui/D0642/2016(H6N6)进行了全基因组序列扩增和测序,以及序列同源性比对和遗传进化分析。结果显示,该分离株的HA基因与野禽源病毒A/turtledove/Wuhan/HKBJ62/2015(H6N6)的核苷酸一致性最高,推导的裂解位点处氨基酸基序为P-Q-I-E-T-R-G,符合低致病性禽流感病毒的分子特征;NA基因无茎部缺失,与早期分离株A/duck/Fujian/6159/2007(H6N6)具有最高的核苷酸相似性;内部基因中,仅NP基因与H6N2亚型禽流感分离株的核苷酸相似性最高,PB2、PB1、PA、M和NS基因均与近几年的H6N6亚型病毒关系紧密。进一步的分析表明,D0642株的8个基因片段均属于欧亚进化谱系,可能由H6N6和H6N2亚型重组产生;并且NA和PB2基因还与当前国内优势流行的2.3.4.4分支H5N6亚型禽流感病毒关系密切,可以为其在自然界的进一步重组提供基因片段的来源。因此,须加强对H6亚型禽流感病毒的流行病学监测。
[Abstract]:A duck H6N6 subtype avian influenza virus (H6N6) isolated from a live poultry market in East China in 2016 was amplified and sequenced, sequence alignment and genetic evolution analysis were performed. The results showed that the nucleotide identity of the HA gene was the highest with that of wild fowl virus A/turtledove/Wuhan/HKBJ62/2015 (H6N6), and the deduced amino acid motif at the cleavage site was P-Q-I-E-T-R-G, which was consistent with the molecular characteristics of the low pathogenic avian influenza virus, and the NA gene had no stem deletion. The nucleotide similarity between NP gene and H6N2 subtype avian influenza isolate was the highest among the internal genes, and PB2,PB1,PA,M and NS genes were closely related to H6N6 subtype virus in recent years. Further analysis showed that the 8 gene fragments of D0642 belonged to the Eurasian phylogeny, which might be produced by the recombination of H6N6 and H6N2 subtypes, and the NA and PB2 genes were also closely related to the prevailing 2.3.4.4 H5N6 subtype avian influenza virus in China. It can provide a source of gene fragments for further recombination in nature. Therefore, epidemiological surveillance of H 6 subtype avian influenza virus should be strengthened.
【作者单位】：扬州大学兽医学院;扬州出入境检验检疫局;江苏高校动物重要疫病与人兽共患病防控协同创新中心;
【基金】：国家重点研发计划项目(2016YFD0500202-1、2016YFD0501601-3) “十二五”农村领域国家科技计划项目(2015BAD12B01-3) 江苏高校优势学科建设工程资助项目(PAPD)
【分类号】：S852.65

【参考文献】