无乳链球菌对体外培养的奶牛乳腺成纤维细胞瘦素及其受体表达的影响

发布时间：2018-10-20 20:26

【摘要】：为研究无乳链球菌对奶牛乳腺成纤维细胞(BMFB)瘦素(Leptin)及其长型受体(OB-Rb)表达的影响,本研究将不同浓度热灭活的无乳链球菌(0、10~5 cfu/mL、10~6 cfu/mL和10~8 cfu/mL)作用于BMFB,分别于不同作用时间采用荧光定量PCR(qPCR)方法检测Leptin及OB-Rb mRNA转录水平,western blot方法检测Leptin及OB-Rb蛋白的表达。各组总体变化规律结果显示,无乳链球菌作用BMFB后,除6 h、12 h 10~6 cfu/m L和10~8 cfu/mL作用浓度外,其余作用时间实验组Leptin mRNA的转录水平及蛋白表达量与对照组相比显著升高(p0.05);各作用时间实验组OB-Rb mRNA的转录水平及蛋白表达量与对照组相比极显著升高(p0.01)。上述研究结果表明,热灭活的无乳链球菌能够促进BMFB的Leptin及OB-Rb mRNA的转录水平和蛋白的表达,为奶牛乳腺纤维化的防治提供依据。
[Abstract]:To study the effect of streptococcus acuminata on the expression of (BMFB) leptin (Leptin) and its long form receptor (OB-Rb) in bovine breast fibroblasts. In this study, the expression of Leptin and OB-Rb proteins were detected by fluorescence quantitative PCR (qPCR) method with different concentrations of heat-inactivated streptococcus actinomycetes (0 ~ 105 cfu/mL,10~6 cfu/mL and 10 ~ (8) cfu/mL) on BMFB, at different time. Leptin and OB-Rb mRNA transcription level, western blot were used to detect the expression of Leptin and OB-Rb, respectively. The results of the total changes of each group showed that, after the treatment of BMFB without streptococcus lactis, the concentration of 10 6 cfu/m L and 10 8 cfu/mL was 10 6 cfu/m / L and 10 8 cfu/mL respectively at 6 h or 12 h after treatment with streptococcus lactis. The transcription level and protein expression of Leptin mRNA in the experimental group were significantly higher than those in the control group (p0.05), and the transcription level and protein expression of OB-Rb mRNA in the experimental group were significantly higher than those in the control group (p0.01). The results showed that the heat-inactivated streptococcus actinomycetes could promote the expression of Leptin and OB-Rb mRNA in BMFB and provide evidence for the prevention and treatment of breast fibrosis in dairy cows.
【作者单位】：内蒙古农业大学兽医学院;
【基金】：国家自然科学基金(31460642)
【分类号】：S858.23

【相似文献】