集约化牛场泌乳奶牛硒缺乏症的调查及血液临床病理学变化
发布时间：2018-10-21 16:24
【摘要】：硒缺乏症是泌乳奶牛的一种重要的营养代谢病。它会引起泌乳期奶牛产奶量降低，免疫功能低下，并继发其他疾病如乳房炎、子宫内膜炎等。近年来，我省集约化牛场奶牛单产不断提高，亚临床代谢病时有发生，危害日趋严重。因此，本实验在两个集约化牛场开展了硒缺乏症的调查，对硒缺乏的奶牛进行了抗氧化、免疫、肝功以及泌乳性能的检测，旨在阐明硒缺乏对集约化牛场高产奶牛健康的影响，为今后高产奶牛硒缺乏症的早期预防提供科学的理论依据。 本试验选择了两个集约化奶牛场(A、B)，分别在泌乳前期（15-20d）、泌乳盛期（50-70d）、泌乳中期（120-150d）和泌乳后期（200-220d）随机选取年龄、体况、泌乳量及胎次相近的奶牛各10头，共80头。通过检测血浆中硒含量，调查A、B两牧场奶牛硒缺乏症的发病率。并根据奶牛血浆中硒水平，选取了缺硒组（30头）与健康对照组（20头），检测试验奶牛的泌乳性能、繁殖性能以及能量代谢（NEFA、BHBA和GLU），抗氧化（GSH-Px、SOD、T-AOC、MDA、T-NOS、LPO、NO、CAT）、免疫功能（TNF-α、IL-6、IgG、IL-1β、IL-2）、肝功（ALB、ALP、TP、GLB、A/G）和生殖激素（FSH、LH、E2、P4），进行Pearson相关性分析和回归分析对牧场进行硒缺乏预警。 研究结果显示：1）牧场A奶牛硒缺乏症的发病率为72.5%，牧场B为77.5%。2）缺硒组与健康对照组相比，奶牛乳脂、乳蛋白及乳糖产量显著的降低（P0.05），初配天数和配种天数明显的延长（P0.05）。3）与健康对照组相比，缺硒组血浆中GSH-Px活性显著的降低，与硒水平呈显著正相关（R=0.36,P=0.01）；MDA、T-NOS和LPO水平明显升高，MDA（R=-0.36,P=0.02）、T-NOS（R=-0.30,P=0.05）、LPO（R=-0.31,P=0.04）与硒水平呈显著负相关。4）与健康对照组相比，缺硒组血浆中IL-1β、IgG、TNF-α和IL-6水平都显著的降低（P0.05），TNF-α（R=0.37,P=0.02）、IL-6（R=0.35,P=0.03）、IgG（R=0.39,P=0.02）、IL-2（R=0.38,P=0.03）与硒水平呈显著正相关。5）与健康对照组相比，缺硒组血浆中ALP、TP水平明显的增加，ALP（R=-0.32,P=0.03）、TP（R=-0.41,P=0.00）与硒水平呈显著负相关。6）与健康对照组相比，缺硒组血浆中P4含量显著的降低，P4（R=0.79,P=0.00）与硒水平呈显著正相关。7）泌乳奶牛血浆中GSH-Px、MDA、IL-2，TNF-α，，IL-6、TP、ALP与硒缺乏密切相关，当GSH-Px水平低于42.367U/mL，则表明该奶牛场硒缺乏症的风险会增加。 结论：1）两个集约化牛场泌乳奶牛普遍存在硒缺乏症，高达72%以上。2）硒缺乏症不仅会影响泌乳奶牛抗氧化能力和免疫功能，而且会降低奶牛泌乳性能和繁殖性能。3）确立了泌乳奶牛硒缺乏时血浆中GSH-Px的风险预警值。
[Abstract]:Selenium deficiency is an important nutritional metabolic disease in lactating cows. It can cause milk production decreased, immune function low, and secondary diseases such as mastitis, endometritis and so on. In recent years, the yield of dairy cows in intensive cattle farms in our province has been increasing, and subclinical metabolic diseases have occurred from time to time, and the harm is becoming more and more serious. Therefore, the investigation of selenium deficiency was carried out in two intensive cattle farms, and the antioxidative, immune, liver function and lactation performance of the dairy cows with selenium deficiency were tested in order to elucidate the effect of selenium deficiency on the health of high-yield cows in intensive cattle farms. To provide scientific theoretical basis for early prevention of selenium deficiency in dairy cows with high yield in the future. In this experiment, two intensive dairy cattle farms (AHB) were selected at the prelactation stage (15-20 d), the peak stage (50-70 d), the middle lactation stage (120-150 d) and the later stage (200-220 d). The incidence of selenium deficiency in dairy cattle was investigated by measuring the selenium content in plasma. According to the level of selenium in the plasma of dairy cows, the lactating performance of the experimental cows was tested by selecting the selenium deficiency group (30) and the healthy control group (20). Reproductive performance and energy metabolism (NEFA,BHBA and GLU), antioxidation (GSH-Px,SOD,T-AOC,MDA,T-NOS,LPO,NO,CAT), immune function (TNF- 伪, IL-6,IgG,IL-1 尾, IL-2), liver function (ALB,ALP,TP,GLB,A/G) and reproductive hormone (FSH,LH,E2,P4), Pearson correlation analysis and regression analysis were used to predict selenium deficiency in pastures. The results showed that: 1) the incidence of selenium deficiency in dairy cattle was 72.5 and 77.5.2 respectively. Milk protein and lactose yield were significantly decreased (P0.05), and the days of initial setting and breeding were significantly prolonged (P0.05). Compared with the healthy control group, the plasma GSH-Px activity of se deficiency group was significantly lower than that of healthy control group, and there was a significant positive correlation between the activity of GSH-Px and selenium level (RP0. 36, P < 0. 01). MDA,T-NOS鍜孡PO姘村钩鏄庢樉鍗囬珮,MDA(R=-0.36,P=0.02),T-NOS(R=-0.30,P=0.05),LPO(R=-0.31,P=0.04)涓庣姘村钩鍛堟樉钁楄礋鐩稿叧.4)涓庡仴搴峰鐓х粍鐩告瘮,缂虹缁勮娴嗕腑IL-1尾,IgG,TNF-伪鍜孖L-6姘村钩閮芥樉钁楃殑闄嶄綆(P0.05),TNF-伪(R=0.37,P=0.02),IL-6(R=0.35,P=0.03),IgG(R=0.39,P=0.02),IL-2(R=0.38,P=0.03)涓庣姘村钩鍛堟樉钁楁鐩稿叧.5)涓庡仴搴峰鐓х粍鐩告瘮, The level of ALP,TP in the plasma of se deficient group was significantly increased, ALP (Ru-0. 32), TP (Ru-0. 41), TP (P0. 00) and se level were negatively correlated. 6) compared with the healthy control group, there was a significant negative correlation between these two groups. P _ 4 content in plasma was significantly decreased in se deficiency group, and P _ 4 (R _ (0.79) P ~ (0. 00) was positively correlated with selenium level. 7) Plasma GSH-Px,MDA,IL-2,TNF- 伪 and IL-6,TP,ALP in lactating cows were closely correlated with selenium deficiency. When GSH-Px level was lower than 42.367 U / mL, the risk of selenium deficiency in the dairy was increased. Conclusion: 1) selenium deficiency is common in two intensive dairy cows, which is more than 72%. 2) selenium deficiency can not only affect the antioxidant capacity and immune function of lactating cows. It also reduced the lactating performance and reproductive performance of dairy cows. 3) the risk early warning value of GSH-Px in plasma was established in lactating cows with selenium deficiency.
【学位授予单位】：黑龙江八一农垦大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.23
本文编号：2285680
[Abstract]:Selenium deficiency is an important nutritional metabolic disease in lactating cows. It can cause milk production decreased, immune function low, and secondary diseases such as mastitis, endometritis and so on. In recent years, the yield of dairy cows in intensive cattle farms in our province has been increasing, and subclinical metabolic diseases have occurred from time to time, and the harm is becoming more and more serious. Therefore, the investigation of selenium deficiency was carried out in two intensive cattle farms, and the antioxidative, immune, liver function and lactation performance of the dairy cows with selenium deficiency were tested in order to elucidate the effect of selenium deficiency on the health of high-yield cows in intensive cattle farms. To provide scientific theoretical basis for early prevention of selenium deficiency in dairy cows with high yield in the future. In this experiment, two intensive dairy cattle farms (AHB) were selected at the prelactation stage (15-20 d), the peak stage (50-70 d), the middle lactation stage (120-150 d) and the later stage (200-220 d). The incidence of selenium deficiency in dairy cattle was investigated by measuring the selenium content in plasma. According to the level of selenium in the plasma of dairy cows, the lactating performance of the experimental cows was tested by selecting the selenium deficiency group (30) and the healthy control group (20). Reproductive performance and energy metabolism (NEFA,BHBA and GLU), antioxidation (GSH-Px,SOD,T-AOC,MDA,T-NOS,LPO,NO,CAT), immune function (TNF- 伪, IL-6,IgG,IL-1 尾, IL-2), liver function (ALB,ALP,TP,GLB,A/G) and reproductive hormone (FSH,LH,E2,P4), Pearson correlation analysis and regression analysis were used to predict selenium deficiency in pastures. The results showed that: 1) the incidence of selenium deficiency in dairy cattle was 72.5 and 77.5.2 respectively. Milk protein and lactose yield were significantly decreased (P0.05), and the days of initial setting and breeding were significantly prolonged (P0.05). Compared with the healthy control group, the plasma GSH-Px activity of se deficiency group was significantly lower than that of healthy control group, and there was a significant positive correlation between the activity of GSH-Px and selenium level (RP0. 36, P < 0. 01). MDA,T-NOS鍜孡PO姘村钩鏄庢樉鍗囬珮,MDA(R=-0.36,P=0.02),T-NOS(R=-0.30,P=0.05),LPO(R=-0.31,P=0.04)涓庣姘村钩鍛堟樉钁楄礋鐩稿叧.4)涓庡仴搴峰鐓х粍鐩告瘮,缂虹缁勮娴嗕腑IL-1尾,IgG,TNF-伪鍜孖L-6姘村钩閮芥樉钁楃殑闄嶄綆(P0.05),TNF-伪(R=0.37,P=0.02),IL-6(R=0.35,P=0.03),IgG(R=0.39,P=0.02),IL-2(R=0.38,P=0.03)涓庣姘村钩鍛堟樉钁楁鐩稿叧.5)涓庡仴搴峰鐓х粍鐩告瘮, The level of ALP,TP in the plasma of se deficient group was significantly increased, ALP (Ru-0. 32), TP (Ru-0. 41), TP (P0. 00) and se level were negatively correlated. 6) compared with the healthy control group, there was a significant negative correlation between these two groups. P _ 4 content in plasma was significantly decreased in se deficiency group, and P _ 4 (R _ (0.79) P ~ (0. 00) was positively correlated with selenium level. 7) Plasma GSH-Px,MDA,IL-2,TNF- 伪 and IL-6,TP,ALP in lactating cows were closely correlated with selenium deficiency. When GSH-Px level was lower than 42.367 U / mL, the risk of selenium deficiency in the dairy was increased. Conclusion: 1) selenium deficiency is common in two intensive dairy cows, which is more than 72%. 2) selenium deficiency can not only affect the antioxidant capacity and immune function of lactating cows. It also reduced the lactating performance and reproductive performance of dairy cows. 3) the risk early warning value of GSH-Px in plasma was established in lactating cows with selenium deficiency.
【学位授予单位】：黑龙江八一农垦大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.23
【引证文献】
相关硕士学位论文 前1条
1 牛聪;黑龙江省部分牛场肉牛营养代谢状况调查及疾病预警[D];黑龙江八一农垦大学;2016年
本文编号：2285680
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