青海省海北州藏系绵羊种羊几种疫病的血清学调查

发布时间：2018-10-22 10:07

【摘要】：为了保证外调藏系种羊不感染疫病及无疫病病原传播,对筛选出的准备调运的藏系种羊进行疫病的血清学调查,试验分别采用布鲁氏菌试管凝集试验、小反刍兽疫病毒抗体直接竞争ELISA方法、口蹄疫病毒非结构蛋白抗体单克隆抗体阻断ELISA方法和口蹄疫O型液相阻断ELISA方法对藏系绵羊30份血清样品进行了布鲁氏菌、小反刍兽疫、口蹄疫自然感染和疫苗免疫的血清学抗体检测。结果表明:所有被检血清中均未检出布鲁氏菌和自然感染口蹄疫病毒抗体阳性血清。小反刍兽疫和口蹄疫疫苗免疫抗体水平检测显示,小反刍兽疫免疫抗体阳性血清30份,阳性率为100%;口蹄疫免疫抗体阳性血清28份、可疑血清1份、阴性血清1份,阳性率为93.33%,阳性血清免疫抗体效价均大于1∶128(保护率大于99.00%),可疑血清免疫抗体效价1∶90(保护率大于50.00%),阴性血清免疫抗体效价小于1∶2(不保护)。说明此次海北州筛选的藏系绵羊种羊未感染上述疫病,且疫苗免疫抗体效价水平较好,适合作为外调种羊。
[Abstract]:In order to ensure the non-infection and no transmission of infectious disease in Tibetan sheep, serological investigation was carried out on the selected Tibetan sheep to be transferred, and the test tube agglutination test of Brucella was used. Brucellosis was performed in 30 sera of Tibetan sheep by ELISA method of direct competition of small ruminant virus antibody, monoclonal antibody against foot-and-mouth disease virus non-structural protein antibody blocking ELISA and liquid phase blocking ELISA method of foot-and-mouth disease (FMD). Detection of serological antibodies against natural infection and vaccine immunization of foot-and-mouth disease. The results showed that Brucella and FMDV antibody positive sera were not detected in all the tested sera. The level of immune antibody against small ruminant and foot-and-mouth disease vaccine showed that 30 sera were positive for small ruminant disease, and the positive rate was 100. 28 sera were positive for FMDV, 1 was suspicious, and 1 was negative. The positive rate was 93.33. The titer of positive serum immune antibody was higher than 1: 128 (the protective rate was more than 99.00%), the antibody titer of suspicious serum was 1:90 (the protective rate was more than 50.00%), and the negative serum immune antibody titer was less than 1:2 (unprotected). The results showed that Tibetan sheep bred in Haibei state were not infected with the above disease, and the antibody titer of vaccine was better, so it was suitable for breeding sheep.
【作者单位】：青海省畜牧兽医科学院;国家绒毛用羊产业技术体系海北综合试验站;青海省海北藏族自治州畜牧兽医科学研究所;
【基金】：国家绒毛用羊产业技术体系项目(CARS-40-09)
【分类号】：S858.26

【相似文献】