蛇床子素体外抗鸽毛滴虫的效果及形态学观察

发布时间：2018-10-23 19:47

【摘要】：为了观察蛇床子素体外抑制鸽毛滴虫的效果及其对形态学的影响,试验分为4组,蛇床子素浓度分别为0(空白)、1、3、5 mg/m L,分别在培养2、4、6、8、12、24 h从各组取样,于光学及电子显微镜下观察鸽毛滴虫的形态学变化,记录死亡情况。结果显示:随着蛇床子素浓度的增加,鸽毛滴虫的死亡率随之升高,死亡时间随之缩短。5 mg/m L组的效果最佳,用药12 h即可杀灭全部的毛滴虫;浓度为1 mg/m L的药物组至24 h仍未全部死亡;浓度为3 mg/m L的药物组至24 h全部死亡。扫描电镜观察显示,药物作用初始阶段鸽毛滴虫由梨形逐步变圆,形态完整;不久虫体边缘模糊,表面出现皱褶,局部有凹陷;接着凹陷面积扩大,内部出现空洞,虫体形状变得不规则,最后虫体崩解成碎片。结果表明蛇床子素具有明显抗鸽毛滴虫的效果。
[Abstract]:In order to observe the inhibitory effect of osthol on Trichomonas pigeon in vitro and its effect on morphology, the experiment was divided into 4 groups: the concentration of osthol was 0 (blank), and the concentration of osthol was 5 mg/m / L. Morphological changes of Trichomonas pigeon were observed under optical and electron microscope, and death was recorded. The results showed that with the increase of osthol concentration, the death rate of Trichomonas pigeon increased and the time of death was shortened. The best effect was in the group of 0.5 mg/m / L, and all trichomonas could be killed after 12 h treatment. The drug group with a concentration of 1 mg/m / L did not die at 24 h, and the drug group with 3 mg/m L did not die at 24 h. Scanning electron microscope observation showed that trichomonas pigeon gradually became round and complete in the initial stage of drug action. Soon, the edge of the insect was blurred, the surface was wrinkled and there was a local depression, and then the area of the depression was enlarged, and the inner cavity appeared. The shape of the worm became irregular, and eventually the insect collapsed into pieces. The results showed that osthol had obvious effect against Trichomonas pigeon.
【作者单位】：南京农业大学动物医学院;金陵科技学院;
【基金】：南京市生物农业项目资助(编号2013-32) 南京市科技计划项目资助(编号201201026)
【分类号】：S858.39

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