绿茶粉和绿茶多酚对犬的抗氧化作用
发布时间：2018-10-24 07:27
【摘要】：本试验旨在探究绿茶粉及绿茶多酚对犬的抗氧化作用。选用5月龄杂交幼犬15只,随机分为3个组,每组5只。对照组饲喂基础饲粮,绿茶粉组饲喂基础饲粮+1.0%的绿茶粉,绿茶多酚组饲喂基础饲粮+0.25%的绿茶多酚。试验期为84 d。结果表明:与试验开始时相比,试验结束时对照组犬体重增长了162%,绿茶粉组犬体重增长了101%,绿茶多酚组犬体重增长了132%,且绿茶粉组和绿茶多酚组试验结束时犬体重显著低于对照组(P0.05),比对照组分别降低了61%和30%。试验第84天,绿茶粉组及绿茶多酚组犬血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)活性均显著或极显著低于对照组(P0.05或P0.01),血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性极显著高于对照组(P0.01),血清丙二醛(MDA)含量极显著低于对照组(P0.01),肝脏抗氧化基因[谷氨酸半胱氨酸连接酶催化亚基(GCLC)、谷氨酸半胱氨酸连接酶修饰亚基(GCLM)、血红素氧合酶1(HO-1)、过氧化氢酶(CAT)]和Ⅱ相解毒酶基因[谷胱甘肽硫转移酶M1(GSTM1)和醌氧化还原酶1(NQO1)]的mRNA相对表达量显著高于对照组(P0.05)。综上所述,在本试验条件下,在犬饲粮中添加1.0%的绿茶粉或0.25%的绿茶多酚均具有保护肝脏、抗氧化、促进Ⅱ相解毒酶基因表达及控制体重的作用。
[Abstract]:The aim of this study was to investigate the anti-oxidation effect of green tea powder and green tea polyphenols on dogs. Fifteen 5 month old crossbred puppies were randomly divided into 3 groups with 5 puppies in each group. The control group was fed with basic diet, the green tea powder group was fed with 1.0% green tea powder and the green tea polyphenol group was fed with 0.25% green tea polyphenol. The experimental period is 84 days. The results show that: compared with the beginning of the experiment, At the end of the experiment, the weight of control group increased 162%, green tea powder group increased 101%, green tea polyphenol group increased 132%, and the weight of green tea powder group and green tea polyphenol group was significantly lower than that of control group at the end of the experiment (P0.05), which decreased by 61% and 30% respectively. On the 84th day of the experiment, The activity of serum alanine aminotransferase (ALT), glutamic oxalacetic transaminase (AST), lactate dehydrogenase (LDH) in green tea powder group and green tea polyphenol group was significantly or extremely lower than that in control group (P0.05 or P0.01), and the activity of serum superoxide dismutase (SOD),) glutathione peroxidase (GSH-Px) was significantly lower than that in control group (P0.01). The content of malondialdehyde (MDA) in serum was significantly lower than that in the control group (P0.01). The liver antioxidant gene [glutamate cysteine ligase catalytic subunit (GCLC), glutamate cysteine ligase modified (GCLM), heme oxygenation] was significantly higher than that of the control group (P0.01). The relative expression of mRNA of enzyme 1 (HO-1), catalase (CAT)] and phase 鈪，
本文编号：2290724
[Abstract]:The aim of this study was to investigate the anti-oxidation effect of green tea powder and green tea polyphenols on dogs. Fifteen 5 month old crossbred puppies were randomly divided into 3 groups with 5 puppies in each group. The control group was fed with basic diet, the green tea powder group was fed with 1.0% green tea powder and the green tea polyphenol group was fed with 0.25% green tea polyphenol. The experimental period is 84 days. The results show that: compared with the beginning of the experiment, At the end of the experiment, the weight of control group increased 162%, green tea powder group increased 101%, green tea polyphenol group increased 132%, and the weight of green tea powder group and green tea polyphenol group was significantly lower than that of control group at the end of the experiment (P0.05), which decreased by 61% and 30% respectively. On the 84th day of the experiment, The activity of serum alanine aminotransferase (ALT), glutamic oxalacetic transaminase (AST), lactate dehydrogenase (LDH) in green tea powder group and green tea polyphenol group was significantly or extremely lower than that in control group (P0.05 or P0.01), and the activity of serum superoxide dismutase (SOD),) glutathione peroxidase (GSH-Px) was significantly lower than that in control group (P0.01). The content of malondialdehyde (MDA) in serum was significantly lower than that in the control group (P0.01). The liver antioxidant gene [glutamate cysteine ligase catalytic subunit (GCLC), glutamate cysteine ligase modified (GCLM), heme oxygenation] was significantly higher than that of the control group (P0.01). The relative expression of mRNA of enzyme 1 (HO-1), catalase (CAT)] and phase 鈪，
本文编号：2290724
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