长沙生态动物园寄生虫调查及动物蛔虫Pcox1序列分析研究
发布时间：2018-10-24 20:35
【摘要】：本次研究在2015年7月采集长沙生态动物园绝大部分动物的粪便,进行了一次寄生虫调查。并从2012年到2015年进行样品采集,共收集到5种蛔虫成虫,分别是驯养东北虎蛔虫样本、白狮蛔虫样本、猎豹蛔虫样本、棕熊(熊)蛔虫样本、斑马蛔虫样本,采用实验室PCR技术对5种蛔虫线粒体coxl扩增测序,再用DNAstar5.0软件进行比对分析,确定5个蛔虫样本的种内种间关系。此研究在野生动物园动物疾病操控中有实用价值,并为野生动物蛔虫的研究和防控提供基础数据。研究结果如下：1.本次调查对77种野生动物粪便进行分类取样,其中27种动物感染寄生虫, 感染率为35.5%。2.经调查发现,球虫、蛔虫、线虫、鞭虫、绦虫、吸虫的感染率依次为2.6%,9.2%,43.4%,10.5%,5.3%,1.3%。3.经调查发现,球虫、蛔虫、线虫、鞭虫、绦虫、吸虫感染强度依次为1-392,1-894,1--280(蛔虫外其他线虫),1-7,1--353,188--298。4.蛔虫的分子线粒体DNA cox1基因片段核苷酸序列分析结果显示：Pcox1序列种内差异较小,种间差异较明显。因此得出结论,Pcox1基因的序列可以作为野生动物蛔虫的遗传标记。5.5种野生动物蛔虫的线粒体coxl系统进化分析驯养东北虎、白狮、猎豹、棕熊(熊)、斑马共5种蛔虫样本,比对系统进化树结果显示：驯养东北虎感染的蛔虫是猫弓首蛔虫,白狮和猎豹感染的蛔虫是狮弓蛔虫,棕熊感染的蛔虫是贝蛔虫,斑马感染的蛔虫是马副蛔虫。6.5种野生动物蛔虫的线粒体coxl基因序列分析结果显示：研究结果显示,5种动物的蛔虫样本Pcox1基因序列的相似性均在95%以上,5种动物的蛔虫样本与GenBank中其他蛔虫相应序列的相似度均低于92%。5种动物的蛔虫样本种间的差异(8.2%～15.6%)远远大于5种动物的蛔虫样本种内的变异(0-5.0%),说明Pcox1能为种间的遗传变异研究提供遗传标记,可用于5种动物的蛔虫样本蛔虫的种间鉴定检测。
[Abstract]:The study collected the feces of most animals from Changsha Ecological Zoo in July 2015 and conducted a parasite survey. From 2012 to 2015, five species of Ascaris lumbricoides were collected. They were domesticated Amur Ascaris, White Lion Ascaris, Cheetah Ascaris, Brown Bear Ascaris, zebra Ascaris. The mitochondrial coxl amplification and sequencing of five Ascaris lumbricoides were carried out by using laboratory PCR technique. The interspecific relationships of five Ascaris lumbricoides samples were determined by comparing and analyzing with DNAstar5.0 software. This study is of practical value in the manipulation of wild animal diseases and provides basic data for the study and prevention and control of Ascaris lumbricoides in wild animals. The results are as follows: 1. A total of 77 species of wild animal faeces were collected and 27 of them were infected with parasites, with an infection rate of 35. 5% and 2. 2%. The infection rate of coccidia, ascariasis, nematode, Trichuris, tapeworm and paragonimiasis was found to be 2.6and 9.2in turn. The results showed that the infection rates of coccidia, Ascaris lumbricoides, nematode, Trichuris, tapeworm and paragonimiasis were 1-392O1-8941-280 (other nematode), 1-71-353188 298.4. The nucleotide sequence analysis of molecular mitochondrial DNA cox1 gene of Ascaris lumbricoides showed that the difference of Pcox1 sequence was small in species and obvious in species. It was concluded that the sequence of Pcox1 gene could be used as a genetic marker of Ascaris lumbricoides in wild animals. 5. 5 species of wild animal Ascaris lumbricoides were collected from 5 species of Ascaris lumbricoides, including Amur tiger, white lion, cheetah, brown bear (bear) and zebra. The comparison of phylogenetic tree results showed that the infected Ascaris was a catworm, the white lion and cheetah infected the Ascaris, and the brown bear infected the Ascaris japonicus. The mitochondrial coxl gene sequence analysis of 6.5 species of wild animal Ascaris lumbricoides showed that the similarity of Pcox1 gene sequence of 5 kinds of animal samples was more than 95%. The similarity between Ascaris lumbricoides and other Ascaris in GenBank was lower than that in 92.5 species of Ascaris lumbricoides (8.2%), which was much larger than that in 5 species (0-5.0%), indicating that Pcox1 could be an interspecific genetic variation. Research provides genetic markers, It can be used for interspecific identification of Ascaris lumbricoides in 5 kinds of animals.
【学位授予单位】：湖南农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S852.7
本文编号：2292482
[Abstract]:The study collected the feces of most animals from Changsha Ecological Zoo in July 2015 and conducted a parasite survey. From 2012 to 2015, five species of Ascaris lumbricoides were collected. They were domesticated Amur Ascaris, White Lion Ascaris, Cheetah Ascaris, Brown Bear Ascaris, zebra Ascaris. The mitochondrial coxl amplification and sequencing of five Ascaris lumbricoides were carried out by using laboratory PCR technique. The interspecific relationships of five Ascaris lumbricoides samples were determined by comparing and analyzing with DNAstar5.0 software. This study is of practical value in the manipulation of wild animal diseases and provides basic data for the study and prevention and control of Ascaris lumbricoides in wild animals. The results are as follows: 1. A total of 77 species of wild animal faeces were collected and 27 of them were infected with parasites, with an infection rate of 35. 5% and 2. 2%. The infection rate of coccidia, ascariasis, nematode, Trichuris, tapeworm and paragonimiasis was found to be 2.6and 9.2in turn. The results showed that the infection rates of coccidia, Ascaris lumbricoides, nematode, Trichuris, tapeworm and paragonimiasis were 1-392O1-8941-280 (other nematode), 1-71-353188 298.4. The nucleotide sequence analysis of molecular mitochondrial DNA cox1 gene of Ascaris lumbricoides showed that the difference of Pcox1 sequence was small in species and obvious in species. It was concluded that the sequence of Pcox1 gene could be used as a genetic marker of Ascaris lumbricoides in wild animals. 5. 5 species of wild animal Ascaris lumbricoides were collected from 5 species of Ascaris lumbricoides, including Amur tiger, white lion, cheetah, brown bear (bear) and zebra. The comparison of phylogenetic tree results showed that the infected Ascaris was a catworm, the white lion and cheetah infected the Ascaris, and the brown bear infected the Ascaris japonicus. The mitochondrial coxl gene sequence analysis of 6.5 species of wild animal Ascaris lumbricoides showed that the similarity of Pcox1 gene sequence of 5 kinds of animal samples was more than 95%. The similarity between Ascaris lumbricoides and other Ascaris in GenBank was lower than that in 92.5 species of Ascaris lumbricoides (8.2%), which was much larger than that in 5 species (0-5.0%), indicating that Pcox1 could be an interspecific genetic variation. Research provides genetic markers, It can be used for interspecific identification of Ascaris lumbricoides in 5 kinds of animals.
【学位授予单位】：湖南农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S852.7
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