硒化党参多糖和大蒜多糖协同增强鸡外周血淋巴细胞和新城疫疫苗的免疫功效

发布时间：2018-10-26 13:52

【摘要】：为了验证硒化党参多糖(selenizing Codonopsis pilosula polysaccharide,sCPP)和大蒜多糖(garlic polysaccharide,GP)的协同增强免疫作用及其复方硒化党参多糖-大蒜多糖(sCPP-GP)的功效,为研制新型免疫增强剂提供理论依据,比较了这3种多糖的增强免疫活性。体外试验,用MTT法和RT-PCR法测定了3种多糖对鸡外周血淋巴细胞的增殖及其IL-2和IFN-γmRNA转录的影响;体内试验,将3种多糖配合新城疫疫苗免疫雏鸡,测定鸡外周血淋巴细胞增殖、血清HI抗体效价、血清IL-2和IFN-γ含量的动态变化。体外试验结果显示,3种多糖在合适的浓度均能显著促进淋巴细胞增殖及IL-2和IFN-γmRNA的转录,复方的作用显著强于两个组分药;体内试验结果显示,在免疫后4个时间点,3种多糖均能不同程度地促进T淋巴细胞增殖,提高血清HI抗体效价、提高血清IL-2和IFN-γ的含量,复方的作用显著强于两个组分药。研究结果表明,sCPP和GP能协同增强鸡外周血淋巴细胞和新城疫疫苗的免疫功效,复方sCPP-GP的作用显著强于两个组分药,可以作为新型免疫增强剂的候选方。
[Abstract]:In order to verify the synergistic immunological effect of Codonopsis selenoides polysaccharide (selenizing Codonopsis pilosula polysaccharide,sCPP) and garlic polysaccharide (garlic polysaccharide,GP) and the efficacy of compound Se-Codone-Codonopsis pilosula polysaccharide garlic polysaccharide (sCPP-GP), In order to provide theoretical basis for the development of new immune enhancers, the enhanced immune activities of these three polysaccharides were compared. In vitro, the effects of three polysaccharides on the proliferation of chicken peripheral blood lymphocytes and the transcription of IL-2 and IFN- 纬 mRNA were determined by MTT and RT-PCR methods. In vivo experiment, three kinds of polysaccharides were immunized with Newcastle disease vaccine to determine the proliferation of peripheral blood lymphocytes, the titer of serum HI antibody, and the dynamic changes of serum IL-2 and IFN- 纬 contents. The results of in vitro experiments showed that the three polysaccharides could significantly promote the proliferation of lymphocytes and the transcription of IL-2 and IFN- 纬 mRNA at the appropriate concentration, and the effect of the compound was significantly stronger than that of the two components. The results of in vivo test showed that at 4 time points after immunization, all three polysaccharides could promote T lymphocyte proliferation, increase the titer of serum HI antibody, and increase the contents of IL-2 and IFN- 纬 in serum. The effect of compound was significantly stronger than that of two components. The results showed that sCPP and GP could synergistically enhance the immune efficacy of chicken peripheral blood lymphocytes and Newcastle disease vaccine, and compound sCPP-GP could be used as a candidate for new immune enhancer.
【作者单位】：南京农业大学中兽医学研究室;
【基金】：国家自然科学基金(31272596) 公益性行业(农业)科研专项(201403051)
【分类号】：S853.74

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