转Bt基因水稻秸秆对小鼠代谢、发育和繁殖的安全性评价

发布时间：2018-10-26 14:14

【摘要】：本研究利用转Bt基因水稻秸秆(秸秆粉与小鼠普通饲料成分按质量比1：3混合配制成饲料)喂养小鼠90d,通过对小鼠的代谢、发育与繁殖等方面的分析,确定Bt蛋白对哺乳动物小鼠的影响,探讨转Bt基因水稻秸秆应用于饲料生产的安全性问题。1.所有小鼠在90天的饲养过程中饮食、饮水和日常行为均表现正常,没有发生中毒或死亡现象；2.血[相、血生化和血液流变学等相关指标,90d小鼠试验组和对照组差异均不显著；3.利用ELISA酶联免疫技术测出90d小鼠血清中CAT、SOD、POD、GSH-Px和AchE酶活,结果发现试验组和对照组差异均不显著；4.利用流式细胞术(FCM)检测90d小鼠血淋巴细胞的凋亡与钙流情况,结果发现无论是细胞凋亡还是钙离子流失试验组与对照组差异均不显著；5.将90d小鼠解剖取主要脏器,观察脏器病变、检测脏器指数,同时利用ELISA酶联免疫技术测定各脏器中CAT、SOD、POD、GSH-Px和AchE酶活,结果发现脏器无病害、毒害情况,各脏器指数和CAT、SOD、POD、GSH-Px、AchE酶活试验组与对照组均无显著性差异；6.取90d雄鼠的精液,测定其精子密度、活力、活率、畸形率和顶体完整率,结果发现试验组与对照组均无明显影响；7.取90d雌鼠的卵巢制作石蜡切片,观察组织病变情况并统计原始卵泡、初级卵泡、次级卵泡和三级卵泡数量,结果发现试验组与对照组卵巢组织没有明显病变情况,各级卵泡数量丰富且无显著性差异。研究表明,转Bt基因水稻秸秆对小鼠没有产生毒害作用,没有引起小鼠各脏器的病变,也没有刺激小鼠体内的关键代谢酶的解毒机制从而引起几种主要酶酶活的变化,并且对于雌性和雄性生殖健康也没有明显有害影响。
[Abstract]:In this study, Bt transgenic rice straw was used to feed mice for 90 days, and the metabolism, development and reproduction of mice were analyzed. To determine the effect of Bt protein on mammalian mice and to explore the safety of transgenic rice straw with Bt gene in feed production. 1. All the mice showed normal diet, drinking water and daily behavior during the 90-day feeding, and no poisoning or death occurred. 2. There was no significant difference in blood phase, blood biochemistry and hemorheology between the test group and the control group. The activities of CAT,SOD,POD,GSH-Px and AchE in serum of 90 day mice were measured by ELISA enzyme-linked immunosorbent assay. The results showed that there was no significant difference between the test group and the control group. 4. Flow cytometry (FCM) was used to detect the apoptosis and calcium flow in blood lymphocytes of 90 day mice. The results showed that there was no significant difference between the experimental group and the control group in both apoptosis and calcium ion loss. 5. The main organs of mice were dissected for 90 days, the pathological changes of organs were observed, and the index of organs was detected. Meanwhile, the activities of CAT,SOD,POD,GSH-Px and AchE in various organs were determined by ELISA enzyme-linked immunosorbent assay. The results showed that there was no disease and toxicity in the organs. There was no significant difference in organ index and CAT,SOD,POD,GSH-Px,AchE enzyme activity between the test group and the control group. 6. The sperm density, motility, viability, deformity rate and acrosomal integrity rate were measured in 90 day male rats. The results showed that there was no significant effect on sperm density, motility, acrosome integrity, and no significant difference between the experimental group and the control group. Paraffin sections were made from the ovaries of 90 d female rats. The pathological changes of the tissues were observed and the number of primary follicles, secondary follicles and tertiary follicles were counted. The results showed that there was no obvious pathological changes in the ovarian tissues between the experimental group and the control group. There was no significant difference in the number of follicles at all levels. The results showed that rice straw transformed with Bt gene had no toxic effect on mice, did not cause changes in various organs of mice, nor did it stimulate the detoxification mechanism of key metabolic enzymes in mice, thus causing changes in the activities of several major enzymes. There were also no significant adverse effects on reproductive health of both females and males.
【学位授予单位】：湖南农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S816

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