羊口疮病程中Th17细胞相关细胞因子的变化
[Abstract]:Sheep contact with infectious pustular dermatitis, or Contagiouts Ecthyma,CE or ORF), is one of the most common diseases in sheep production in the world. It is widely used in Jilin, Ningxia, Yunnan, Henan and Hubei provinces in China. Xinjiang and Shaanxi provinces have reported that goats are more susceptible to infection than sheep, and the incidence of goat farms is usually 10% and 100%. Although the disease itself rarely leads to adult goat death, but to the newborn lamb serious harm, infection of mouth and lips caused by serious ulcers affect the feeding of lambs, and eventually lead to death; In addition, the disease also endangers the productive performance of adult sheep, causing mastitis and other diseases. The clinical features of sheep mouth ulcers include papules, blisters, pustules, skin rupture and finally scab, which is a typical inflammatory reaction. What kinds of cells and cytokines are involved in the process of this inflammation have not been reported yet. In this study, histopathological techniques, lymphocyte proliferation, real-time PCR and prokaryotic expression were used to study the characteristics of epithelial lesions in the course of sheep mouth ulcers. The healthy goat peripheral blood mononuclear lymphocytes (Peripheral blood mononuclear cells,PBMCs) were stimulated by the strong and weak strains of sheep mouth sore, and the proliferation of PBMCs and the level of cytokine mRNA were detected. At the same time, the levels of cytokine mRNA and the expression of IL-1 尾 and IL-6 protein were detected in sheep infected with aphtha. The results are as follows: 1. The main histological changes of skin injury caused by sheep mouth sore were vacuolar degeneration of epithelial cells and infiltration of inflammatory cells in subbasal connective tissue. With the development of the course of disease, the number of infiltrating cells increased, and the number of infiltrating cells was the most in pemphigus stage, and the number of infiltrating cells decreased gradually after entering the convalescent stage. Infiltrating cells mainly include lymphocytes, neutrophils, macrophages and other immune cells. The infiltration of inflammatory cells was mainly neutrophilic granulocyte in the early stage of the lesion, lymphocyte infiltration in blister and pemphigus, and lymphocyte and macrophage in convalescent stage. 2. Sheep mouth sore was strong and attenuated strains could significantly stimulate goat PBMCs proliferation. The effect of virulent strain was better than that of attenuated strain. The levels of IL-17 mRNA, IL-1 尾, IL-6 and IL-23 in PBMCs increased significantly, while TGF- 尾 mRNA decreased significantly. The level of cytokine mRNA produced by PBMCs in sheep mouth sore course showed that the levels of IL-1 尾, IL-6 and IL-23 mRNA in vesicular phase were significantly increased, IL-17 mRNA level in pemphigus phase was significantly increased, and IFN- 纬 mRNA level was also significantly increased. 3. Goat IL-1 尾 and IL-6 genes were cloned. The target fragments were 463 bp and 552 bp, prokaryotic expression fusion proteins, respectively. The size of IL-1 尾 and IL-6 fusion proteins were 37.4 kDa and 40.6 kDa, respectively. The polyclonal antibodies were prepared by immunizing rabbits. The titers of serum antibodies determined by Agar double diffusion were 1:16 and 1: 8, respectively. The results show that: 1. The pathological process of sheep mouth ulcer is essentially immune pathological reaction. The immune cells involved in this process are neutrophils at the early stage and lymphocytes at the later stage. The main cytokine that mediates the immune pathological reaction of sheep mouth sore is IL. According to the results of other cytokines measured in this study, we speculated that the IL 17 in sheep aphthous ulcer reaction originated from Th17 cells, in addition, Th1 cell subsets were also involved in the inflammatory reaction of sheep aphtha. 3. The cloning and expression of goat IL 1 尾, IL 6 protein and antiserum provide the preparation for further study of the immune pathological mechanism of sheep aphthous ulcer in our laboratory.
【学位授予单位】:西北农林科技大学
【学位级别】:硕士
【学位授予年份】:2015
【分类号】:S858.27
【相似文献】
相关期刊论文 前10条
1 刘玉芬,刘洪雨,张奎武;羊口疮的诊断与治疗[J];黑龙江畜牧兽医;2004年10期
2 王炳清;;山羊口疮的诊治体会[J];畜牧兽医杂志;2008年04期
3 鲜思美;胡廷帅;熊朝丽;向智龙;陈秀华;;贵州省羊口疮的调查研究[J];动物医学进展;2012年11期
4 李前瑞;李杰;田婷婷;姚运亮;许君艳;赵燕青;陈德坤;张淼涛;;羊口疮病毒多重PCR检测方法的建立[J];动物医学进展;2013年06期
5 蒋建;;羊口疮临床病理与防治[J];当代畜牧;2014年20期
6 罗燕雯,陈兴芳,王玉智,马爱荣,李平,高金梅;用犊牛睾丸细胞生产羊口疮疫苗时应注意的问题[J];中国兽药杂志;2003年07期
7 张廷胜;;碘酊治疗羊口疮[J];中兽医学杂志;2007年01期
8 任宝忠;夏会秋;王淑娟;;羊口疮病的防治[J];畜牧与饲料科学;2007年05期
9 颜新敏;张强;;羊口疮病毒的研究进展[J];中国动物检疫;2008年11期
10 王树春;周关发;李志华;高林生;;中西医结合治疗羔羊口疮[J];云南畜牧兽医;2008年02期
相关会议论文 前2条
1 何水林;陈杰;蔡玉书;姚龙涛;;羊口疮病毒的分离与鉴定[A];第四届中国畜牧兽医青年科技工作者学术研讨会论文集[C];2001年
2 刘远;李文杨;林仕欣;高承芳;张晓佩;董晓宁;;福建省羊口疮的流行病学调查与PCR诊断[A];中国畜牧兽医学会养羊学分会2012年全国养羊生产与学术研讨会议论文集[C];2012年
相关重要报纸文章 前3条
1 华坪县畜牧兽医站 杨永军;不可小视羊口疮病[N];云南科技报;2013年
2 张国华;浅析山羊口疮的综合防治措施[N];中国畜牧兽医报;2006年
3 ;综合防治山羊口疮[N];中国畜牧兽医报;2007年
相关硕士学位论文 前10条
1 杨雯昱;羊口疮病程中Th17细胞相关细胞因子的变化[D];西北农林科技大学;2015年
2 田婷婷;羊口疮疫苗研制及其免疫效果评估[D];西北农林科技大学;2013年
3 张瑜;羊口疮病毒杨凌株和泸西株的分离鉴定及毒力致弱研究[D];西北农林科技大学;2014年
4 张立强;羊口疮病原分子流行病学调查及蜂胶佐剂灭活疫苗免疫效果研究[D];西北农林科技大学;2014年
5 高晶晖;羊口疮病毒威海株和昆明株的分离鉴定及毒力致弱研究[D];西北农林科技大学;2014年
6 贾云晓;富平某奶山羊养殖场羊口疮分子病原学调查及灭活疫苗免疫效果评估[D];西北农林科技大学;2014年
7 刘方;杨凌地区羊口疮流行病学调查、病毒分离鉴定及相关基因的生物信息学分析[D];西北农林科技大学;2014年
8 李杰;羊口疮抗体ELISA检测方法的建立及应用[D];西北农林科技大学;2013年
9 孔汉金;羊口疮病毒感染宿主细胞的蛋白质组变化及功能分析[D];华中农业大学;2014年
10 李浩;不同地区羊口疮病毒三个主要结构蛋白基因的遗传进化分析[D];内蒙古农业大学;2015年
,本文编号:2298245
本文链接:https://www.wllwen.com/yixuelunwen/dongwuyixue/2298245.html