日粮蛋白水平对生长育肥猪肠道微生物酶活性的影响
发布时间：2018-10-29 19:14
【摘要】：为探讨日粮粗蛋白(crude protein,CP)水平对生长育肥猪肠道微生物胞内酶活性的影响,试验分别选择体质量分别为(30±2.54),(60±3.18)kg的杜×长×大三元杂交猪各18头,均采用单因子完全随机分组设计分配到CP为12%,15%,18%和10%,13%,16%的3组日粮中,每组6个重复,每个重复1头猪。预试期7d,正试期30d。在正饲期31d,每组随机挑选6头进行屠宰,采集各肠段食糜和粪便样品,研究日粮CP水平对生长育肥猪肠道微生物胞内酶活性的影响。结果表明,生长育肥猪肠道微生物酶活力均具有肠段特异性;日粮CP水平能显著影响猪肠道微生物硝酸还原酶(P0.05)、谷氨酰胺合成酶(P0.05)、谷草转氨酶(P0.05)、谷丙转氨酶(P0.05)、乳酸脱氢酶(P0.05)、尿素酶(P0.05)及蛋白酶(P0.05)水平;日粮CP水平对肠道微生物的蛋氨酸、谷氨酸、精氨酸、酪氨酸、色氨酸脱羧酶活力影响显著(P0.05);日粮CP水平显著影响肠道微生物酶的活力,是肠道微生物对氨基酸差异性降解和动物对蛋白质利用率低的原因之一。
[Abstract]:In order to investigate the effect of dietary crude protein (crude protein,CP) level on the intracellular enzyme activity of intestinal microbes in growing and fattening pigs, 18 DU 脳 Chang 脳 Da three-way crossbred pigs with a body weight of (30 卤2.54), (60 卤3.18) kg were selected respectively. All of them were divided into 3 groups with CP of 12 15% and 10 15% and 16% respectively by a single factor complete randomized grouping design. Each group had 6 repeats and 1 pig per replica. The pretest period was 7 days and the current test period was 30 days. On the first day of feeding, 6 pigs in each group were randomly selected for slaughter. The effects of dietary CP level on the intracellular enzyme activity of intestinal microbes in growing and fattening pigs were studied. The results showed that the intestinal microbial enzyme activity of growing and fattening pigs had intestinal segment specificity. Dietary CP levels significantly affected intestinal nitrate reductase (P0.05), glutamine synthase (P0.05), aspartate aminotransferase (P0.05), alanine aminotransferase (P0.05), lactate dehydrogenase (P0.05). The levels of urease (P0.05) and protease (P0.05); Dietary CP levels significantly affected the activities of methionine, glutamate, arginine, tyrosine and tryptophan decarboxylase in intestinal microbes (P0.05). Dietary CP level significantly affected the activity of intestinal microbial enzymes, which was one of the reasons for the different degradation of amino acids by intestinal microbes and the low utilization of protein in animals.
【作者单位】： 西南大学生物饲料与分子营养实验室;
【基金】：国家“973”重点基础研究发展计划资助项目(2013CB127303) 国家“948”计划资助项目(2015Z74) 重庆市自然科学基金资助项目(cstc2016jcyjA1414) 西南大学基本科研业务费资助项目(XDJK2015D005,XDJK2016D010,XDJK2014B028)
【分类号】：S828.5
[Abstract]:In order to investigate the effect of dietary crude protein (crude protein,CP) level on the intracellular enzyme activity of intestinal microbes in growing and fattening pigs, 18 DU 脳 Chang 脳 Da three-way crossbred pigs with a body weight of (30 卤2.54), (60 卤3.18) kg were selected respectively. All of them were divided into 3 groups with CP of 12 15% and 10 15% and 16% respectively by a single factor complete randomized grouping design. Each group had 6 repeats and 1 pig per replica. The pretest period was 7 days and the current test period was 30 days. On the first day of feeding, 6 pigs in each group were randomly selected for slaughter. The effects of dietary CP level on the intracellular enzyme activity of intestinal microbes in growing and fattening pigs were studied. The results showed that the intestinal microbial enzyme activity of growing and fattening pigs had intestinal segment specificity. Dietary CP levels significantly affected intestinal nitrate reductase (P0.05), glutamine synthase (P0.05), aspartate aminotransferase (P0.05), alanine aminotransferase (P0.05), lactate dehydrogenase (P0.05). The levels of urease (P0.05) and protease (P0.05); Dietary CP levels significantly affected the activities of methionine, glutamate, arginine, tyrosine and tryptophan decarboxylase in intestinal microbes (P0.05). Dietary CP level significantly affected the activity of intestinal microbial enzymes, which was one of the reasons for the different degradation of amino acids by intestinal microbes and the low utilization of protein in animals.
【作者单位】： 西南大学生物饲料与分子营养实验室;
【基金】：国家“973”重点基础研究发展计划资助项目(2013CB127303) 国家“948”计划资助项目(2015Z74) 重庆市自然科学基金资助项目(cstc2016jcyjA1414) 西南大学基本科研业务费资助项目(XDJK2015D005,XDJK2016D010,XDJK2014B028)
【分类号】：S828.5
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