L-精氨酸和α-酮戊二酸对热应激肉鸡肠道吸收功能、抗氧化能力、能量代谢的影响
发布时间:2018-10-31 06:57
【摘要】:本试验旨在研究L-精氨酸(Arg)和α-酮戊二酸(AKG)对热应激肉鸡肠道功能的影响。选用200只1日龄Cobb肉鸡,随机分为4个处理组:对照组(基础日粮)、热应激组(基础日粮)、Arg组(基础日粮+0.5%Arg)、AKG组(基础日粮+0.5%AKG),每个组5个重复,每个重复10只鸡。试验为期5周,第1周各组室温控制在32~34℃,第2~5周热应激组、Arg组和AKG组控制在(36±2)℃,而对照组第2周开始慢慢降至(22±2)℃,并从第3周开始维持在(22±2)℃,湿度为50%~60%。结果表明:与对照组相比,热应激处理降低了肉鸡生长性能和血液三碘甲腺原氨酸(T3)含量(P0.05),同时也显著降低了空肠和回肠脂肪酶、回肠胰蛋白酶、空肠过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)、回肠超氧化物歧化酶(SOD)和CAT的活力、十二指肠和回肠三磷酸腺苷(ATP)含量、空肠小肽转运载体(Pep T1)、紧密连接蛋白(Claudin-5)m RNA表达量(P0.05),显著提高了死亡率、血液皮质酮、十二指肠和回肠丙二醛(MDA)含量、空肠低氧诱导因子-1α(HIF-1α)、AMP依赖的蛋白激酶α1(AMPK-α1)m RNA表达量(P0.05);与热应激组相比,日粮中添加0.5%Arg可显著提高热应激肉鸡饲料转化率、十二指肠脂肪酶和回肠胰蛋白酶活力、十二指肠和空肠DNA含量、空肠CAT活力和HMOX-1 m RNA表达量(P0.05),降低死亡率、十二指肠和空肠RNA/DNA比值、空肠TP/DNA比值、HIF-1α和AMPK-α1 m RNA表达量(P0.05),而日粮中添加0.5%AKG可显著提高热应激肉鸡血液T3、十二指肠和空肠DNA含量、十二指肠CAT和GSH-Px的活力、十二指肠和空肠及回肠ATP含量(P0.05),并显著降低死亡率、血液皮质酮含量、空肠RNA/DNA及TP/DNA比值、十二指肠MDA和回肠过氧化氢(H2O2)含量(P0.05)。因此,日粮中添加0.5%Arg可提高热应激肉鸡肠道消化能力和抗氧化功能,而添加0.5%AKG可促进热应激肉鸡肠道黏膜细胞生长,提高肠道抗氧化能力,并改善肠道能量代谢状况。
[Abstract]:The purpose of this study was to study the effects of L-arginine (Arg) and 伪 -ketoglutarate (AKG) on intestinal function of heat stressed broilers. Two hundred 1-day-old Cobb broilers were randomly divided into four groups: control group (basal diet), heat stress group (basal diet), Arg group (basal diet 0.5%Arg), AKG group), basal diet 0.5%AKG group). There were 5 repeats in each group and 10 chickens in each group. The experiment lasted for 5 weeks. The room temperature of each group was controlled at 32 鈩,
本文编号:2301322
[Abstract]:The purpose of this study was to study the effects of L-arginine (Arg) and 伪 -ketoglutarate (AKG) on intestinal function of heat stressed broilers. Two hundred 1-day-old Cobb broilers were randomly divided into four groups: control group (basal diet), heat stress group (basal diet), Arg group (basal diet 0.5%Arg), AKG group), basal diet 0.5%AKG group). There were 5 repeats in each group and 10 chickens in each group. The experiment lasted for 5 weeks. The room temperature of each group was controlled at 32 鈩,
本文编号:2301322
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