鸡性成熟启动前后下丘脑microRNA表达谱分析

发布时间：2018-11-01 12:08

【摘要】：本研究旨在探讨miRNA在鸡性成熟启动调控中的作用。利用Solexa高通量测序技术检测了鸡性成熟启动前(Before puberty onset,BPO)和性成熟启动后(After puberty onset,APO)下丘脑中miRNA表达谱,通过生物信息学方法进行靶基因预测和功能分析。结果表明,在鸡下丘脑中鉴定出374个已知miRNAs、46个新miRNAs,144个已知miRNAs(reads10)的表达水平在性成熟启动前后发生了显著改变(P0.05)。5个差异表达miRNAs被qRT-PCR方法进一步验证。15个差异最显著miRNA(|log2(fold-change)|2.0,P0.01)预测到2 013个靶基因,GO富集和KEGG调节通路分析结果表明,这些差异表达miRNAs在性成熟启动转录调节和信号转导通路中发挥了重要作用。综上表明,miRNA是参与鸡性成熟启动新的调节因子。鉴于miRNA功能的保守性,该研究结果将为深入开展动物(包括人类)性成熟的分子调控机制研究提供新的理论依据。
[Abstract]:The purpose of this study was to investigate the role of miRNA in the regulation of sexual maturation initiation in chickens. The expression profiles of miRNA in hypothalamus of chicken before and after sexual maturation were detected by Solexa high-throughput sequencing technique. Target gene prediction and functional analysis were performed by bioinformatics. The results showed that 374 known miRNAs, were identified in chicken hypothalamus and 46 new miRNAs, were identified. The expression level of 144 known miRNAs (reads10) changed significantly before and after the initiation of sexual maturation (P0.05). 5 differentially expressed miRNAs were further verified by qRT-PCR method. The 15 most significant miRNA (log2 (fold-change) 2.0), P0.01) predicted 2013 target genes. The results of GO enrichment and KEGG regulation pathway analysis showed that these differentially expressed miRNAs played an important role in the initiation of transcriptional regulation and signal transduction pathway in sexual maturation. It is concluded that miRNA is a new regulatory factor involved in the initiation of sexual maturation in chickens. In view of the conserved nature of miRNA function, this study will provide a new theoretical basis for the further study of the molecular regulation mechanism of sexual maturity in animals (including humans).
【作者单位】：中国农业科学院家禽研究所国家级地方鸡种基因库;
【基金】：国家自然科学基金(31201799) 江苏省农业科技创新项目(CX[15]1009)
【分类号】：S831

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