益生菌发酵复方中药产物治疗鸡大肠杆菌病效果研究

发布时间：2018-11-03 15:15

【摘要】：鸡大肠杆菌病是由大肠杆菌引起的一种传染病,具有发病率高、死亡率高的特点,严重影响养鸡业发展。所以如何防控大肠杆菌病是亟待解决的问题。目前防治该病主要依靠大量使用抗生素,容易造成菌群失调、产生耐药性和药物残留,极大影响鸡产品质量,}f食品安全造成极大隐患。本研究应用益生菌发酵中药技术,对益生菌复方制剂进行筛选,对复方中药进行配比和筛选,进行发酵液、中药液体外抑菌试验,并用对照组、感染组、中药组、发酵液组、西药组对鸡大肠杆菌病进行治疗和对比,用药三天后,测定SIgA、病理组织切片、抗氧化指标,通过整理数据分析治疗效果差异性。结果显示保加利亚乳杆菌与植物乳杆菌(1:4)复方制剂OD值最大,明显高于保加利亚乳杆菌、植物乳杆菌。选用18味中药组成抗大肠杆菌的复方制剂:黄芪20g,黄连10g,乌梅10g,白头翁30g,茯苓30g,黄芩20g,金银花20g,大青叶10g,葛根30g,千里光20g,神曲15g,甘草20g,山楂15g,泽泻10g,诃子10g,明矾10g,黄柏10g,麦芽25g,总计315g。发酵条件:温度37℃,时间48h,转速200rpm,接种量20mL,装液量480mL。发酵液体外抑菌实验的抑菌圈直径为25mm,中药体外抑菌实验的抑菌圈直径为20mm,发酵液体外抑菌效果要高于中药抑菌效果。病理组织切片结果显示发酵液组试验鸡的十二指肠、盲肠病理损伤情况明显轻于中药组和西药组;SIgA检测结果表明,发酵液组鸡盲肠内容物中SIgA水平高于西药组,与中药组差异不显著,发酵液组鸡十二指肠内容物中SIgA水与其他各组差异不明显。血清丙二醛含量检测结果表明发酵液组丙二醛含量高于中药组和西药组;血清谷胱甘肽过氧化物酶含量结果表明,发酵液组该酶含量明显高于中药组和西药组;肝脏超氧化物歧化酶浓度检测结果表明发酵液组高于西药组,而低于中药组;血清超氧化物酶浓度检测结果表明发酵液组高于西药组和中药组。结论:通过中药组、西药组、发酵液组体外抑菌试验、临床动物试验、指标检测试验,确定发酵液对大肠杆菌的治疗效果好于中药治疗效果。
[Abstract]:E. coli disease is an infectious disease caused by Escherichia coli, which has the characteristics of high incidence and high mortality, which seriously affects the development of chicken industry. Therefore, how to prevent and control Escherichia coli disease is an urgent problem to be solved. At present, the prevention and treatment of the disease mainly rely on the use of a large number of antibiotics, which is easy to cause bacteriological disorders, drug resistance and drug residues, greatly affect the quality of chicken products,} f food safety caused great hidden trouble. In this study, probiotic fermentation Chinese medicine technology was used to screen probiotic compound formulations, mixture and screening of compound Chinese medicine, fermentation broth, liquid external bacteriostatic test of traditional Chinese medicine, and control group, infection group, traditional Chinese medicine group, fermentation broth group, and the control group, infection group, Chinese medicine group, fermentation broth group. The treatment and comparison of chicken colibacillosis were carried out in the western medicine group. After three days of treatment, the pathological sections of SIgA, and the antioxidant index were determined, and the difference of treatment effect was analyzed by sorting out the data. The results showed that the OD value of the compound preparation of Lactobacillus bulgaricus and Lactobacillus plantarum (1:4) was higher than that of Lactobacillus bulgaricus and Lactobacillus plantarum. 18 Chinese herbs were used to make up the compound preparation of anti-Escherichia coli: astragalus 20g, Coptis chinensis 10g, Wupai 10g, wurin 30g, Scutellaria baicalensis 20g, honeysuckle 20g, big blue leaf 10g, kudzu root 30g, Qianliguang 20g, Shenqu 15g, licorice 20g, Hawthorn 15g. Alisma 10g, Chebula chebula 10g, alum 10g, juniper 10g, malt 25g, total 315g. The fermentation conditions were as follows: temperature 37 鈩，

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