主成分分析法与聚类分析法在胎衣不下奶牛血液生化研究中的应用

发布时间：2018-11-06 15:32

【摘要】：通过分析胎衣不下奶牛血液生化指标的变化特征,为奶牛胎衣不下早期诊断方法的建立提供依据。产犊后立即无菌采集47头奶牛血液,其中25头为胎衣正常排出奶牛,22头为胎衣不下奶牛;血液生化分析仪检测血清生化指标,主成分分析法与聚类分析法分析检测结果。结果表明,16项血清生化指标被聚类成3个主成分,主成分聚类分析的结果与临床采集样品的分类结果一致;与胎衣正常排出奶牛相比,胎衣不下奶牛的血清中乳酸脱氢酶、谷草转氨酶、谷氨酰胺转移酶、肌酸激酶水平显著升高。表明,主成分分析和聚类分析可以有效地区别胎衣不下奶牛血清和胎衣正常排出奶牛血清,因此可以通过此方法对奶牛产后胎衣不下的发生进行提前诊断;还表明奶牛胎衣不下的发生不仅与蛋白质代谢、离子代谢和肌肉代谢紊乱有关,而且肝脏和肾脏的机能损伤也可能导致胎衣不下。
[Abstract]:The changes of blood biochemical indexes were analyzed to provide basis for the establishment of early diagnosis method. The blood samples of 47 cows were collected immediately after calving, of which 25 cows were normally excreted by fetal coat and 22 cows were uncoated cows. The serum biochemical indexes were detected by blood biochemistry analyzer, and the results of principal component analysis and cluster analysis were analyzed. The results showed that 16 serum biochemical indexes were clustered into three principal components, and the results of principal component cluster analysis were consistent with those of clinical samples. The levels of lactate dehydrogenase, glutamic oxaloacetic transaminase, glutamine transferase and creatine kinase in serum were significantly higher than those in cows with normal excretion of fetal coat, and the levels of lactate dehydrogenase, aspartate aminotransferase and creatine kinase were significantly increased. The results showed that the principal component analysis and cluster analysis could effectively distinguish the serum from the normal excretion of cow serum, so it could be used to diagnose the occurrence of postpartum fetal coat in advance. It is also suggested that the occurrence of fetal coat is not only related to the disorder of protein metabolism, ion metabolism and muscle metabolism, but also the damage of liver and kidney may lead to fetal coat failure.
【作者单位】：中国农业科学院兰州畜牧与兽药研究所甘肃省中兽药工程技术研究中心;
【基金】：公益性行业(农业)科研专项(201303040) 国家奶牛产业技术体系(CARS-37-06)~~
【分类号】：S858.23

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