来源于鸡肠道的乳酸菌的筛选及其饲用性的评价

发布时间：2018-11-12 11:10

【摘要】：为给益生菌的制备提供良好的株菌来源,自健康成年鸡肠道内分离鉴定得到9株(S1、S2、K1、K2、K3、H1、H2、M1、M2)乳酸菌,从生长速度、耐酸、耐胆盐、耐鸡胆汁、抑菌能力、安全性等方面对株菌进行初步筛选。结果表明:S1、S2、K3和M14株菌接种后8 h快速生长,进入对数生长期,接种22 h后进入细菌生长稳定期。耐酸试验表明S1、S2、K3和M1表现为较强的耐酸能力,其中M1耐酸能力最强,在p H为2.0、3.0、4.0的培养基中存活率分别为76.8%、79.2%、91.5%。耐胆盐试验表明S1、S2、K3和M1 4株在0.1%、0.2%、0.3%浓度的胆盐培养基中的存活率分别为93.5%、89.6%、86.3%;96.1%、90.3%、86.7%;94.8%、88.7%、85.6%;95.2%、91.1%、85.3%。在0.1%、0.2%、0.3%浓度的胆汁培养基中S1、S2、K3和M1的存活率分别为93.8%、90.5%、87.1%;95.9%、90.8%、86.8%;95.6%、88.7%、85.8%;94.8%、91.7%、86.2%。S2和M1对大肠杆菌的抑菌圈直径为16.23和18.25mm,表现为高度敏感;S1和K3对大肠杆菌的抑菌圈直径为11.68和12.58mm,表现为中度敏感。
[Abstract]:In order to provide a good source for the preparation of probiotics, nine strains of lactic acid bacteria were isolated from the intestinal tract of healthy adult chickens. The bacteriostatic ability and safety of the strain were preliminarily screened. The results showed that S1S2K3 and M14 strains grew rapidly 8 h after inoculation, entered logarithmic growth stage, and entered stable growth period after 22 h inoculation. The acid tolerance test showed that S1S2K3 and M1 showed strong acid tolerance, among which M1 had the strongest acid tolerance, and the survival rate of M1 was 76.8% 79.2% and 91.5%, respectively, when pH was 2.0 ~ 3.0 ~ 4.0. The results of bile salt tolerance test showed that the survival rate of S1S2K3 and M11 strains in 0.1% and 0.2% concentration bile salt medium was 93.510% and 89.6%, respectively. The survival rate of S1S2OK3 and M11 strain was 96.3% and 96.3%, respectively, and the survival rate of S1S2K3 and M11 strains was 86.7%. The score of 88.7 is 85.6 and the part of 95.1 is about 85.3. The survival rates of S1S2K3 and M1 in the bile medium of 0.2wt% and 0.3wt% were 93.8and 90.5and 87.1respectively. The survival rate of S1S2K3 and M1 was 90.880 and 86.8, respectively. The diameter of the inhibition circle of S.2 and M1 to Escherichia coli was 16.23 mm and 18.25 mm, respectively, and the diameter of the inhibition circle of S.2 and M1 was 16.23 mm and 18.25 mm, respectively. The bacteriostasis diameters of S1 and K3 to Escherichia coli were 11.68 mm and 12.58 mm, showing moderate sensitivity.
【作者单位】：北京农业职业学院畜牧兽医系;
【基金】：北京市教育委员会科技计划面上项目禽源益生菌添加剂的研发与推广(KM201512448006)
【分类号】：S816.3

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