SPF级豚鼠饲料中VC的添加与饲料辐照效果的研究
[Abstract]:Due to strict control of microorganism, restriction of feeding conditions, standardization and large-scale production and breeding of SPF grade guinea pigs, it is necessary to adopt full-price degreening feed which is easy to operate and sterilize. In this study, the addition of VC and irradiation sterilization in SPF grade guinea pig forage were studied, and three experiments were carried out. The results are as follows: 1. Taking ordinary guinea pig as experimental object, ethyl cellulose coated with VC and monomer VC was used as the main source of vitamin C in guinea pig degreening feed, and the feasibility test was carried out by using ethyl cellulose coated with VC and monomer VC as the main source of vitamin C in guinea pig degreening feed. The results showed that the degreening feed with ethyl cellulose coated with VC and monomer VC could basically meet the need of growth and reproduction of guinea pigs, and the effect of VC coated with ethyl cellulose was better. So it is feasible to use ethyl cellulose coated with VC as the main source of vitamin C in guinea pig forage. 2. The experiment was carried out on guinea pigs of SPF grade with the addition of ethyl cellulose coated with VC of 500 mg/kg,1000 mg/kg,1500 mg/kg, respectively, in order to optimize the amount of ethyl cellulose coated with VC. The results showed that 1000 mg/kg,1500 mg/kg was more beneficial to the growth and reproduction of SPF grade guinea pigs than 500 mg/kg, and the addition of 1500 mg/kg had no significant difference compared with 1000 mg/kg in the growth and reproduction index. So combined with feed cost, select 1000 mg/kg as the optimal addition amount. 3. The weight and organ coefficient of main organs of SPF guinea pigs and normal guinea pigs fed with two kinds of feed were compared, and the suitability of SPF grade guinea pigs was further determined. 4. In order to be used in SPF grade guinea pigs, degreening forage must be sterilized by irradiation, so the effect of feed on growth and reproduction of guinea pigs after 15 kGy,30kGy,45 kGy irradiation sterilization was compared. The results showed that the bacteria growth appeared in the feed sterilized by 15 kGy irradiation dose, and the preservation period was not long enough. After 30 kGy irradiation sterilization, the feed had long storage life and was fed to SPF grade guinea pigs, and the growth and reproduction status was stable. After 45 kGy of sterilized feed, the feeding condition of guinea pigs was poor because of the odour, and the growth and reproduction index showed difference and instability. Therefore, 30 kGy was determined as the best irradiation dose of SPF grade guinea pig forage, and the storage time was 8 months. By analyzing the effects of VC addition and irradiation dose on growth and reproduction of guinea pigs, it was found that the effects of VC addition and irradiation dose on the growth and reproduction of guinea pigs were interacted. The change of growth and reproduction index of guinea pigs is that irradiation dose can change the VC coated with ethyl cellulose through the action of degreening feed, and then affect the growth and reproduction of SPF grade guinea pigs.
【学位授予单位】:兰州理工大学
【学位级别】:硕士
【学位授予年份】:2016
【分类号】:S816
【相似文献】
相关期刊论文 前3条
1 高勤卫;李明子;董晓;;乙基纤维素共混改性聚乳酸的研究[J];南京林业大学学报(自然科学版);2006年01期
2 张岩,陈岚,李保国,华泽钊,伍贻文,刘哲鹏,陆伟跃;超临界CO_2喷雾干燥制备乙基纤维素微粒的实验研究[J];农业工程学报;2004年05期
3 ;[J];;年期
相关会议论文 前7条
1 沈大娲;马林;康宏亮;于慧;黄勇;;乙基纤维素接枝聚丙烯酸叔丁酯的合成与表征[A];2005年全国高分子学术论文报告会论文摘要集[C];2005年
2 陈日清;王春鹏;储富祥;;乙基纤维素丙烯酸酯杂化乳液的制备[A];第九届中国林业青年学术年会论文摘要集[C];2010年
3 刘文勇;刘瑞刚;康宏亮;吴敏;黄勇;;乙基纤维素接枝聚丙烯酸共聚物在水体系中的自组装[A];中国化学会第26届学术年会功能高分子科学前沿分会场论文集[C];2008年
4 严春晓;朱海燕;周朝华;陈艺曦;;乙基纤维素包覆活性炭微粒的制备与表征[A];第五届全国工业催化技术与应用年会论文集(上册)[C];2008年
5 李洪青;邹华生;刘会冲;;乙基纤维素合成用稀释剂的实验研究[A];第九届全国化学工艺学术年会论文集[C];2005年
6 袁伟忠;张锦春;邹辉;沈天翔;;具有双侧链的两亲性乙基纤维素梳型共聚物:简易合成、自组装与可调温度-pH响应性[A];2011年全国高分子学术论文报告会论文摘要集[C];2011年
7 袁清华;张志英;董知之;吴世臻;王曙光;王冬梅;肖长发;;乙基纤维素修饰的PEO薄膜的等温结晶动力学[A];第八届功能性纺织品及纳米技术研讨会论文集[C];2008年
相关硕士学位论文 前5条
1 周桂娥;聚合物乙基纤维素膜材料研制及高效液相色谱法在其性能研究中的应用[D];安徽大学;2003年
2 杨猛;乙基纤维素手性膜的制备及分离性能的研究[D];云南师范大学;2008年
3 张磊;几种吸附剂对大鼠血内毒素及炎症因子吸附作用的比较[D];中南大学;2007年
4 姚从春;乙基纤维素气体复合膜的制备与改性研究[D];大连理工大学;2013年
5 简春梅;基于乙基纤维素/壳聚糖的刺激响应性超分子体系的构筑[D];清华大学;2014年
,本文编号:2332163
本文链接:https://www.wllwen.com/yixuelunwen/dongwuyixue/2332163.html