转化生长因子β对体外成熟猪卵子成熟率的影响
发布时间：2018-11-15 09:09
【摘要】：在猪卵子的体外生产方面,关于卵子的体外成熟和体外受精技术目前还存在着不足,特别是猪的受精卵体外生产方面存在复杂的细胞质成熟、多精子侵入率较高以及前核形成受到抑制等问题。为改善猪卵子体外成熟体系,将转化生长因子β(transforming growth factor-β,简称TGFβ)添加到体外成熟的培养液中,研究TGFβ对裸卵和卵丘卵母细胞复合体成熟率的影响。在没有添加TGFβ的体外成熟培养液中,裸卵和卵丘卵母细胞复合体被成熟培养24 h,结果没有卵子达到M-Ⅱ时期,但是在添加TGFβ的体外成熟培养液中,观察到有卵子达到M-Ⅱ时期。另一方面,当卵丘卵母细胞复合体在体外成熟培养时,将TGFβ添加到不同培养阶段(0~24 h或24~48 h)的培养液时,卵子的成熟率没有差别。对裸卵在体外成熟培养24 h时,当在前半期添加TGFβ时(0~24 h),卵子的成熟率为59%;当在后半期添加TGFβ时(24~48 h),卵子的成熟率为57%。同样在裸卵组中,当在全期添加TGFβ时(0~48 h),卵子的成熟率为27%;当无添加TGFβ时,卵子的成熟率为38%。前2组与后2组相比,卵子成熟率有显著差异。
[Abstract]:In the field of in vitro production of porcine eggs, there are still deficiencies in vitro maturation and fertilization techniques, especially in the complex cytoplasmic maturation of pig fertilized eggs. The rate of polysperm invasion is high and the formation of anterior nucleus is inhibited. In order to improve the in vitro maturation system of porcine eggs, transforming growth factor 尾 (transforming growth factor- 尾 (TGF 尾) was added to the culture medium to study the effect of TGF 尾 on maturation rate of naked eggs and cumulus oocyte complexes. In vitro maturation medium without TGF 尾, nake-oocyte and cumulus oocyte complexes were cultured for 24 h, and no eggs reached M- 鈪，
本文编号：2332858
[Abstract]:In the field of in vitro production of porcine eggs, there are still deficiencies in vitro maturation and fertilization techniques, especially in the complex cytoplasmic maturation of pig fertilized eggs. The rate of polysperm invasion is high and the formation of anterior nucleus is inhibited. In order to improve the in vitro maturation system of porcine eggs, transforming growth factor 尾 (transforming growth factor- 尾 (TGF 尾) was added to the culture medium to study the effect of TGF 尾 on maturation rate of naked eggs and cumulus oocyte complexes. In vitro maturation medium without TGF 尾, nake-oocyte and cumulus oocyte complexes were cultured for 24 h, and no eggs reached M- 鈪，
本文编号：2332858
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