番鸭中网状内皮组织增生症病毒感染的检测

发布时间：2018-11-15 09:34

【摘要】：网状内皮组织增生症病毒(reticuloendotheliosis virus,REV)是一种可感染多品种禽的免疫抑制病病原,禽类感染REV后会导致机体发生免疫抑制并由此引发多种病原的继发感染(或混合感染),给养禽业造成极大损失。本研究利用针对REV基因组长末端重复序列(long terminal repeat,LTR)的特异性引物,对21份2013年收集的福建(12份)和浙江(9份)两省的番鸭源病料(肝脏和脾脏组织)进行检测。其中来源于福建漳州(1株,记为REV-FJ-MD01)和浙江宁波(1株,记为REV-ZJ-MD01)的番鸭源病料检测到REV感染阳性。将目的片段经克隆后进行序列测定分析发现,REV-FJ-MD01和REV-ZJ-MD01在该扩增区域的核苷酸同源性为100%,与GenBank中的REV参考株(除鸽源REV和鸭源713株仅1个碱基差异外)核苷酸同源性也为100%;从遗传进化关系可以看出,REV-FJ-MD01和REV-ZJ-MD01与REV参考株遗传进化较近,处于相同的遗传进化分支。本研究首次证实在福建番鸭中存在REV感染。
[Abstract]:Reticuloendotheliosis virus (reticuloendotheliosis virus,REV) is a pathogen of immunosuppressive disease that can infect many species of poultry. Infection of REV in poultry can lead to immunosuppression and lead to secondary infection (or mixed infection) of many pathogens. The poultry industry has caused great losses. In this study, 21 samples of muscovy duck (liver and spleen) from Fujian (12) and Zhejiang (9) were collected in 2013 by using specific primers targeting the long terminal repeat (long terminal repeat,LTR) of REV genome. Among them, Muscovy duck samples from Zhangzhou (1 strain, REV-FJ-MD01) and Ningbo (1 strain, REV-ZJ-MD01) from Zhejiang Province were found to be positive for REV infection. The nucleotide homology of REV-FJ-MD01 and REV-ZJ-MD01 in the amplified region was 100. The nucleotide homology of REV reference strain in GenBank (except for only one base difference between pigeon REV and duck 713 strain) was 100. It can be seen from the genetic evolution relationship that the genetic evolution of REV-FJ-MD01 and REV-ZJ-MD01 is close to that of REV reference strains, and they are in the same branch of genetic evolution. This study first confirmed the presence of REV infection in Fujian Muscovy Duck.
【作者单位】：福建省农业科学院畜牧兽医研究所/福建省畜禽疫病防治工程技术研究中心/福建省禽病防治重点实验室;
【基金】：国家水禽产业技术体系项目(CARS-43) 福建省畜禽疫病防控技术重大研发平台(2014N2003) 福建省优良蛋鸭品种与设施化养殖创新产业化工程项目(2014-2016) 福建省农业科学院畜牧兽医研究所基金[MYQJ2015(S)-3]
【分类号】：S858.32

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