MYNN基因在猪不同组织及肌肉中的表达规律

发布时间：2018-11-18 13:13

【摘要】：试验旨在研究myoneurin(MYNN)基因在猪不同组织中的表达特征及其在肌肉(背最长肌、股二头肌和腰大肌)、小脑、肝脏、胰脏、肾脏、胃、脾脏、肺脏组织中的发育性表达规律。采用实时荧光定量PCR技术研究猪MYNN mRNA在90日龄大白猪和马身猪的心脏、肝脏、脾脏、肺脏、肾脏、小脑、小肠、胰脏、胃、股二头肌及脂肪共11个组织中的表达谱,以及在大白猪和马身猪1、90、180日龄3个发育阶段的肌肉、小脑、肝脏、胰脏、肾脏、胃、脾脏、肺脏组织中的发育性表达规律。结果表明,MYNN在猪的各种组织中广泛表达,且各组织间表达差异显著或极显著(P0.05;P0.01);MYNN在大白猪和马身猪的肌肉、小脑、肝脏、胰脏、肾脏、胃、脾脏、肺脏组织中的不同发育阶段表达差异显著或极显著(P0.05;P0.01),并具有特定规律,由此推测其可能在猪的这几种组织中发挥重要作用。MYNN基因的表达与组织、日龄及品种的遗传背景有关。本试验为研究猪MYNN基因的生物学功能提供了依据,但还需要深入的研究来探索其作用的具体机制,尤其是在骨骼肌发育中的调节机制。
[Abstract]:The purpose of this study was to investigate the expression characteristics of myoneurin (MYNN) gene in different tissues of pigs and its developmental expression in muscle (longissimus dorsi, biceps femoris and psoas major), cerebellum, liver, pancreas, kidney, stomach, spleen and lung. The expression profiles of porcine MYNN mRNA in heart, liver, spleen, lung, kidney, cerebellum, small intestine, pancreas, stomach, biceps femoris and fat were studied by real-time fluorescence quantitative PCR. And the developmental expression of muscle, cerebellum, liver, pancreas, kidney, stomach, spleen and lung in three developmental stages of 1: 90180 days old in white pig and horse body pig. The results showed that MYNN was widely expressed in all kinds of pig tissues, and there were significant or extremely significant differences among the tissues (P0.05, P0.01). The expression of MYNN in muscle, cerebellum, liver, pancreas, kidney, stomach, spleen and lung was significantly different (P0.05). The expression of MYNN gene is related to the genetic background of tissue, age and variety. This study provides a basis for the study of the biological function of porcine MYNN gene, but it needs to be further studied to explore the specific mechanism of its action, especially the regulatory mechanism in skeletal muscle development.
【作者单位】： 山西农业大学动物科技学院;
【基金】：山西省科技创新重点团队项目(201605D131045-24) 三晋学者项目(2016) 山西省农业科技攻关项目(20140311020-5)
【分类号】：S828
，

本文编号：2340123