日粮蛋白水平对仔猪肝脏氨基酸代谢转化的影响研究
发布时间：2018-11-18 18:41
【摘要】：本论文旨在研究日粮蛋白水平对仔猪肝脏氨基酸代谢转化的影响规律及机制,包括以下四个研究内容：研究内容一：日粮蛋白水平对仔猪肝门静脉氨基酸浓度及门静脉净吸收的影响。选择18头体重为20±1.0 kg的杜×长×大三元杂交猪,分别安装门静脉、颈动脉、肠系膜静脉和肝静脉血插管,按单因子完全随机设计分配到粗蛋白(CP)为20%、17%、14%的三组日粮中,每组6个重复。动物自由饮水,每日于0800、1600、2400 h进行饲喂。待动物手术恢复后分别于0730、0930、1130、1430 h采集肝静脉、门静脉和颈动脉血液样品。结果表明,除谷氨酸(Glu)外,门静脉其他氨基酸的浓度均高于肝静脉(P0.05)；仔猪门静脉、肝静脉血浆总氨基酸(TAA)和非必需氨基酸(NEAA)浓度以及肝静脉血浆必需氨基酸(EAA)浓度随日粮蛋白水平的降低而显著降低(P0.05),但是门静脉血浆EAA浓度不受日粮蛋白水平的影响(P0.05)；20%、17%、14%蛋白组门静脉血浆EAA浓度与NEAA浓度的比值分别为1.21、1.23、1.24,明显高于肝静脉中的相应比值(P0.05)。降低日粮蛋白水平显著降低门静脉TAA、NEAA的净吸收量(P0.05),但是对门静脉EAA的净吸收量无显著影响(P0.05)；20%、17%、14%蛋白质组门静脉EAA净吸收与NEAA净吸收的比值分别为1.70、2.08和2.06。氨基酸经过肝脏后TAA的绝对变化量不受日粮蛋白水平的影响(P0.05), NEAA的绝对变化量随日粮蛋白水平的降低而显著降低(P0.05),而EAA的绝对变化量随日粮蛋白水平的降低而显著升高(P0.05)；在肝脏中EAA绝对变化量与NEAA绝对变化量的比值随日粮蛋白水平的降低而升高(P0.05)；经过肝脏代谢后,Glu流通量有显著增加。研究内容二：日粮蛋白水平对仔猪物质代谢的影响,试验设计同试验一。结果表明,随着日粮蛋白水平的增加,门静脉与肝静脉血浆葡萄糖(GLU)和血尿素氮(BUN)浓度显著上升(P0.05),但是日粮蛋白水平对颈动脉GLU和BUN浓度无显著影响(P0.05)。日粮蛋白水平对仔猪门静脉、肝静脉及颈动脉总蛋白浓度无显著影响(P0.05)。研究内容三：日粮蛋白水平对仔猪肝脏氨基酸代谢转化酶的影响。选择54头28日龄的杜×长×大三元杂交断奶仔猪,采用单因子完全随机设计分配到CP为20%、17%、14%的三组日粮中。试验预饲期为7 d,正试期为45 d。在正试期的第10 d、第25 d及第45 d,分别从每组挑选6头仔猪进行屠宰,采集肝脏样品。结果表明,仔猪肝脏谷丙转氨酶、谷草转氨酶及谷氨酰胺合成酶的活力随日粮蛋白水平的增加而显著上升(P0.05),日粮蛋白水平对仔猪肝脏谷氨酸脱氢酶活力无显著影响(P0.05)。研究内容四,日粮蛋白水平对仔猪肝脏氨基酸转运载体表达的影响。试验设计同研究内容三。结果表明,氨基酸转运载体SLC6A15、SLC6A20、SLC36A1及SLC38A2 mRNA表达量随蛋白水平的增加而升高。小结：本研究表明,除了适当的降低日粮蛋白水平及补充EAA外,还需补充特定的NEAA在日粮中的比例。这不仅可以降低氮的排放,维持氨基酸代谢转化酶活力和氨基酸转运载体的表达,还可以提高仔猪肝脏氨基酸代谢转化效率。
[Abstract]:The purpose of this study is to study the effect of daily grain protein level on the transformation of liver amino acid metabolism in piglets, including the following four studies: the effect of the level of daily grain protein on the amino acid concentration of the portal vein and the net absorption of the portal vein in the piglets. The portal vein, the carotid artery, the mesenteric vein and the hepatic venous blood tube were respectively installed at 18 head weight of 20 to 1. 0kg. The total random design of the single factor was 20%, 17% and 14% of the three groups of daily food, and 6 repeats in each group. The animals were fed with free drinking water at 0800, 1600 and 2400 hours a day. The hepatic vein, portal vein and carotid blood samples were collected at 0730, 0930, 1130, and 1430h after the recovery of the animal. The results showed that, in addition to glutamic acid (Glu), the concentration of other amino acids in the portal vein was higher than that of the hepatic vein (P0.05); the portal vein of the piglets, The concentration of total amino acid (TAA) and non-essential amino acid (NEAA) in the hepatic vein and the concentration of essential amino acid (EAA) in the plasma of the hepatic vein decreased significantly with the decrease of the daily food protein level (P0.05), but the plasma EAA concentration in the portal vein was not affected by the protein level of the day (P0.05); 20%, The ratio of the concentration of EAA to the concentration of NEAA in the portal vein of the 14% protein group was 1.21, 1.23, and 1.24, respectively, which was higher than that in the hepatic vein (P0.05). The net absorption of TAA and NEAA in the portal vein was significantly lower than that of the portal vein (P0.05), but the net absorption of the portal vein (EAA) was not significantly affected (P0.05). The ratio of the net absorption of the portal vein (EAA) and the net absorption of the NEAA in the portal vein (EAA) of the portal vein (EAA) was 1.70, 2.08 and 2.06, respectively. The absolute change of TAA in the liver of the amino acid was not affected by the dietary protein level (P0.05). The absolute change of the NEA decreased significantly with the decrease of the daily food protein level (P0.05), while the absolute change of the EAA was significantly increased with the decrease of the daily food protein level (P0.05). The ratio of the absolute change of the EAA and the absolute change of the NEA in the liver increased with the decrease of the daily food protein level (P0.05); after the liver metabolism, the turnover of Glu increased significantly. Study content 2: The effect of daily grain protein level on the metabolism of the pig's substance and the design of the test were the same as that of the experiment. The results showed that the plasma glucose (GLU) and blood urea nitrogen (BUN) concentration in the portal vein and the hepatic vein were significantly increased with the increase of daily food protein level (P0.05), but the level of daily grain protein had no significant effect on the concentration of GLU and BUN in the carotid artery (P0.05). The level of daily grain protein had no significant effect on the total protein concentration of portal vein, hepatic vein and carotid artery (P0.05). The effect of dietary protein on the metabolism of amino acid in the liver of piglets was studied. A total of 20%, 17% and 14% of the three-day diets with a single factor of 20%, 17% and 14% were randomly assigned to the weaned piglets at the age of 54 to 28 days. The pre-feeding period of the trial was 7 days, and the positive test period was 45 days. 6 piglets were selected from each group for slaughter and the liver samples were collected during the 10th day, the 25th day and the 45th day of the positive test period. The results showed that the activity of the glutamic-pyruvic transaminase, the aspartate aminotransferase and the amino-aminopeptidase in the liver of the piglets increased significantly with the increase of the daily grain protein level (P0.05), and the dietary protein level had no significant effect on the activity of the liver glutamate dehydrogenase of the piglets (P0.05). The effect of dietary protein on the expression of amino acid transporter in piglets was studied. The test design is the same as the study content. The results showed that the expression of SLC6A15, SLC6A20, SLC36A1 and SLC38A2 mRNA increased with the increase of protein level. Summary: This study shows that, in addition to the proper reduction of dietary protein levels and the addition of EAA, the proportion of specific NEAA in daily food needs to be supplemented. The invention not only can reduce the emission of nitrogen, maintain the activity of the amino acid metabolism invertase and the expression of the amino acid transport vector, but also improve the conversion efficiency of the amino acid metabolism of the liver of the piglets.
【学位授予单位】：西南大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S828.5
本文编号：2340834
[Abstract]:The purpose of this study is to study the effect of daily grain protein level on the transformation of liver amino acid metabolism in piglets, including the following four studies: the effect of the level of daily grain protein on the amino acid concentration of the portal vein and the net absorption of the portal vein in the piglets. The portal vein, the carotid artery, the mesenteric vein and the hepatic venous blood tube were respectively installed at 18 head weight of 20 to 1. 0kg. The total random design of the single factor was 20%, 17% and 14% of the three groups of daily food, and 6 repeats in each group. The animals were fed with free drinking water at 0800, 1600 and 2400 hours a day. The hepatic vein, portal vein and carotid blood samples were collected at 0730, 0930, 1130, and 1430h after the recovery of the animal. The results showed that, in addition to glutamic acid (Glu), the concentration of other amino acids in the portal vein was higher than that of the hepatic vein (P0.05); the portal vein of the piglets, The concentration of total amino acid (TAA) and non-essential amino acid (NEAA) in the hepatic vein and the concentration of essential amino acid (EAA) in the plasma of the hepatic vein decreased significantly with the decrease of the daily food protein level (P0.05), but the plasma EAA concentration in the portal vein was not affected by the protein level of the day (P0.05); 20%, The ratio of the concentration of EAA to the concentration of NEAA in the portal vein of the 14% protein group was 1.21, 1.23, and 1.24, respectively, which was higher than that in the hepatic vein (P0.05). The net absorption of TAA and NEAA in the portal vein was significantly lower than that of the portal vein (P0.05), but the net absorption of the portal vein (EAA) was not significantly affected (P0.05). The ratio of the net absorption of the portal vein (EAA) and the net absorption of the NEAA in the portal vein (EAA) of the portal vein (EAA) was 1.70, 2.08 and 2.06, respectively. The absolute change of TAA in the liver of the amino acid was not affected by the dietary protein level (P0.05). The absolute change of the NEA decreased significantly with the decrease of the daily food protein level (P0.05), while the absolute change of the EAA was significantly increased with the decrease of the daily food protein level (P0.05). The ratio of the absolute change of the EAA and the absolute change of the NEA in the liver increased with the decrease of the daily food protein level (P0.05); after the liver metabolism, the turnover of Glu increased significantly. Study content 2: The effect of daily grain protein level on the metabolism of the pig's substance and the design of the test were the same as that of the experiment. The results showed that the plasma glucose (GLU) and blood urea nitrogen (BUN) concentration in the portal vein and the hepatic vein were significantly increased with the increase of daily food protein level (P0.05), but the level of daily grain protein had no significant effect on the concentration of GLU and BUN in the carotid artery (P0.05). The level of daily grain protein had no significant effect on the total protein concentration of portal vein, hepatic vein and carotid artery (P0.05). The effect of dietary protein on the metabolism of amino acid in the liver of piglets was studied. A total of 20%, 17% and 14% of the three-day diets with a single factor of 20%, 17% and 14% were randomly assigned to the weaned piglets at the age of 54 to 28 days. The pre-feeding period of the trial was 7 days, and the positive test period was 45 days. 6 piglets were selected from each group for slaughter and the liver samples were collected during the 10th day, the 25th day and the 45th day of the positive test period. The results showed that the activity of the glutamic-pyruvic transaminase, the aspartate aminotransferase and the amino-aminopeptidase in the liver of the piglets increased significantly with the increase of the daily grain protein level (P0.05), and the dietary protein level had no significant effect on the activity of the liver glutamate dehydrogenase of the piglets (P0.05). The effect of dietary protein on the expression of amino acid transporter in piglets was studied. The test design is the same as the study content. The results showed that the expression of SLC6A15, SLC6A20, SLC36A1 and SLC38A2 mRNA increased with the increase of protein level. Summary: This study shows that, in addition to the proper reduction of dietary protein levels and the addition of EAA, the proportion of specific NEAA in daily food needs to be supplemented. The invention not only can reduce the emission of nitrogen, maintain the activity of the amino acid metabolism invertase and the expression of the amino acid transport vector, but also improve the conversion efficiency of the amino acid metabolism of the liver of the piglets.
【学位授予单位】：西南大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S828.5
【引证文献】
相关期刊论文 前2条
1 杨静;许庆庆;李貌;陈澄;黄飞若;吴赵亮;孙志洪;;饲粮粗蛋白质水平对仔猪门静脉回流组织氮代谢的影响[J];中国畜牧杂志;2016年21期
2 孙志洪;李貌;许庆庆;印遇龙;朱伟云;江青艳;黄飞若;;猪氨基酸代谢节俭机制新假说[J];动物营养学报;2016年11期
相关硕士学位论文 前2条
1 杨静;甘氨酸和丙氨酸在猪肝脏中的代谢去向研究[D];西南大学;2016年
2 吴赵亮;不同生长阶段猪门静脉排流组织氨基酸代谢规律研究[D];西南大学;2016年
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