酶法辅助超声提取功劳木中小檗碱的工艺优化
发布时间：2018-11-19 17:01
【摘要】：为了优化酶法辅助超声提取功劳木中小檗碱的工艺,在单因素试验基础上,根据BoxBehnken中心组合试验设计原理,采用4因素3水平的响应面分析法,以小檗碱收率为响应值进行回归分析。结果表明,酶解最佳工艺条件为:酶解pH值3.5、酶用量10.6 mg/g、酶解温度51℃、酶解时间97 min。在此条件下,小檗碱收率为22.46 mg/g。优选的提取工艺稳定可行、收率高,为功劳木的工业化生产提供参考。
[Abstract]:In order to optimize the enzymatic ultrasonic extraction process of berberine from Gongrou, based on the single factor test and the design principle of BoxBehnken center combination test, a response surface analysis method with 4 factors and 3 levels was used. Using berberine yield as response value, regression analysis was carried out. The results showed that the optimum conditions of enzymatic hydrolysis were as follows: the pH value of enzymatic hydrolysis was 3.5, the enzyme dosage was 10.6 mg/g, the hydrolysis temperature was 51 鈩，
本文编号：2342894
[Abstract]:In order to optimize the enzymatic ultrasonic extraction process of berberine from Gongrou, based on the single factor test and the design principle of BoxBehnken center combination test, a response surface analysis method with 4 factors and 3 levels was used. Using berberine yield as response value, regression analysis was carried out. The results showed that the optimum conditions of enzymatic hydrolysis were as follows: the pH value of enzymatic hydrolysis was 3.5, the enzyme dosage was 10.6 mg/g, the hydrolysis temperature was 51 鈩，
本文编号：2342894
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