应用Illumina-MiSeq高通量测序技术分析脱氧雪腐镰刀菌烯醇对小鼠肠道菌群的影响
发布时间：2018-11-19 17:19
【摘要】：本试验旨在研究脱氧雪腐镰刀菌烯醇(DON)对小鼠肠道微生物多样性、物种丰度的影响。选用平均体重为20 g的BALB/c小鼠40只,随机分成对照组(灌胃灭菌生理盐水)和DON组(灌胃1.8 mg/kg BW DON),每组20个重复,每个重复1只小鼠,连续灌服28 d。试验结束后,收集新鲜粪便,每组随机选取3份,用Illumina-Mi Seq高通量测序技术分析微生物群落结构和组成的变化。结果表明,1)与对照组相比,DON组测序数量减少(P0.05)。2)DON组降低了Alpha、Beta多样性,Shannon指数较对照组显著降低(P0.05)。3)在门水平,与对照组相比,DON组显著降低了拟杆菌门(Bacteroidetes)和脱铁杆菌门(Deferribacteres)的物种丰度(P0.05),显著提高了变形菌门(Proteobacteria)的物种丰度(P0.05)。在属水平,与对照组相比,DON组显著降低了副类杆菌属(Parabacteroides)、理研菌属(Rikenella)、Algoriphagus、Mucispirillum、嗜甲基菌属(Methylophilus)、Francisella的物种丰度(P0.05),显著提高了梭菌属(Clostridium)、Robinsoniella、A llobaculum、A kkermansia的物种丰度(P0.05)。4)聚类分析显示,DON组与对照组的肠道微生物相似性降低。由此可见,DON能显著影响小鼠肠道微生物菌群多样性及物种丰度,提示DON致肠道损伤与这些肠道菌群的变化密切相关。
[Abstract]:The purpose of this study was to study the effects of deoxynivalenol (DON) on the microbial diversity and species abundance in the intestinal tract of mice. Forty BALB/c mice with an average body weight of 20 g were randomly divided into two groups: control group (normal saline by gastric administration) and DON group (20 replicates in each group of 1.8 mg/kg BW DON), by gavage for 28 days). At the end of the experiment, fresh feces were collected and three samples were randomly selected from each group. The changes of microbial community structure and composition were analyzed by Illumina-Mi Seq high-throughput sequencing technique. The results showed that: 1) compared with the control group, the number of DON sequencing decreased (P0.05). The DON group decreased the Alpha,Beta diversity, and the Shannon index significantly decreased compared with the control group (P0.05). DON group significantly decreased the species abundance of (Bacteroidetes) and (Deferribacteres) (P0.05), and significantly increased the species abundance of (Proteobacteria) (P0.05). At the generic level, compared with the control group, the DON group significantly decreased the species abundance of the genus (Parabacteroides), Rhinopsis, (Rikenella), Algoriphagus,Mucispirillum, (Methylophilus), Francisella (P0.05), and significantly increased the species abundance of the genus (Clostridium), Robinsoniella,. Cluster analysis of species abundance of A llobaculum,A kkermansia (P0.05) showed that the similarity of intestinal microbes between DON group and control group was decreased. It can be seen that DON can significantly affect the diversity and species abundance of intestinal microflora in mice, suggesting that the intestinal injury induced by DON is closely related to the changes of these intestinal flora.
【作者单位】： 上海市农业科学院农产品质量标准与检测技术研究所;
【基金】：上海市农委科技兴农重点攻关项目[沪农科攻字(2013)第3-8号] 上海市农委青年人才成长计划[沪农青字(2015)第1-33号]
【分类号】：S859.8
，
本文编号：2342934
[Abstract]:The purpose of this study was to study the effects of deoxynivalenol (DON) on the microbial diversity and species abundance in the intestinal tract of mice. Forty BALB/c mice with an average body weight of 20 g were randomly divided into two groups: control group (normal saline by gastric administration) and DON group (20 replicates in each group of 1.8 mg/kg BW DON), by gavage for 28 days). At the end of the experiment, fresh feces were collected and three samples were randomly selected from each group. The changes of microbial community structure and composition were analyzed by Illumina-Mi Seq high-throughput sequencing technique. The results showed that: 1) compared with the control group, the number of DON sequencing decreased (P0.05). The DON group decreased the Alpha,Beta diversity, and the Shannon index significantly decreased compared with the control group (P0.05). DON group significantly decreased the species abundance of (Bacteroidetes) and (Deferribacteres) (P0.05), and significantly increased the species abundance of (Proteobacteria) (P0.05). At the generic level, compared with the control group, the DON group significantly decreased the species abundance of the genus (Parabacteroides), Rhinopsis, (Rikenella), Algoriphagus,Mucispirillum, (Methylophilus), Francisella (P0.05), and significantly increased the species abundance of the genus (Clostridium), Robinsoniella,. Cluster analysis of species abundance of A llobaculum,A kkermansia (P0.05) showed that the similarity of intestinal microbes between DON group and control group was decreased. It can be seen that DON can significantly affect the diversity and species abundance of intestinal microflora in mice, suggesting that the intestinal injury induced by DON is closely related to the changes of these intestinal flora.
【作者单位】： 上海市农业科学院农产品质量标准与检测技术研究所;
【基金】：上海市农委科技兴农重点攻关项目[沪农科攻字(2013)第3-8号] 上海市农委青年人才成长计划[沪农青字(2015)第1-33号]
【分类号】：S859.8
，
本文编号：2342934
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