抗生素对仔猪肠道微生物的影响

发布时间：2018-11-19 18:42

【摘要】：本研究旨在从肠道微生物的角度探究抗生素的作用机制,为今后绿色抗生素替代品添加剂的研究提供理论基础和参考。试验选用6头30日龄的杜×长×大三元杂交去势公猪,随机分为2组,分别为基础饲粮组(不添加抗生素)和抗生素组(基础饲粮中添加0.12%复合抗生素),每组3个重复,每个重复1头猪。试验期为35 d。结果表明:抗生素组肠道微生物物种多样性较基础饲粮组更为丰富;抗生素组厚壁菌门(Firmicute)/拟杆菌门(Bacteroidetes)(F/B)比例较高,因此添加抗生素有利于仔猪体内脂肪的贮存;从肠道微生物的角度考虑,抗生素可能是通过促进L7A_E11、Flexispira、粪球菌属(Coprococcus)等菌属,抑制光岗菌属(Mitsuokella)、巨球菌属(Megasphaera)、柔嫩菌属(Faecalibacterium)等菌属从而发挥预防疾病、提高成活率和促进生长的作用;使用PICRUSt进行功能预测,抗生素在脂类生物合成蛋白质、甲烷代谢、转运蛋白、转录因子、转录机制、RNA转运和脂肪酸生物合成等通路中发挥了重要的作用。由此可见,抗生素可以通过调节仔猪肠道微生物,加速仔猪体内脂肪的贮存,加速断奶仔猪对粗纤维的降解作用,促进短链脂肪酸的生成,从而发挥预防疾病、提高成活率和促进生长等作用。
[Abstract]:The purpose of this study is to explore the mechanism of antibiotics from the point of view of intestinal microorganism, and to provide theoretical basis and reference for the study of green antibiotic substitute additives in the future. Six 30-day-old ovariectomized boars were randomly divided into two groups: basal diet group (without antibiotics) and antibiotic group (basal diet supplemented with 0.12% compound antibiotics). There were 3 replicates in each group and 1 pig in each group. The experimental period is 35 days. The results showed that the species diversity of intestinal microorganism in antibiotic group was more abundant than that in basal diet group. The ratio of (Firmicute) / (Bacteroidetes) (F / B in antibiotic group was higher, so the addition of antibiotics was beneficial to the storage of fat in piglets. From the point of view of intestinal microbes, antibiotics may prevent diseases by promoting L7AE11Flexis pirae, faeces, (Coprococcus), (Mitsuokella), (Megasphaera), (Faecalibacterium), and so on. Increasing survival rate and promoting growth; Using PICRUSt for functional prediction, antibiotics play an important role in lipid biosynthesis proteins, methane metabolism, transporter, transcription factors, transcriptional mechanisms, RNA transport and fatty acid biosynthesis. It can be seen that antibiotics can accelerate the storage of fat in piglets, accelerate the degradation of crude fiber in weaned piglets, promote the formation of short chain fatty acids, and thus play a role in the prevention of diseases by regulating intestinal microbes of piglets, accelerating the storage of fat in piglets, and accelerating the degradation of crude fiber in weaned piglets. Increase survival rate and promote growth and so on.
【作者单位】：扬州大学动物科学与技术学院;太仓市金诸农业发展有限公司;江苏徐淮地区淮阴农业科学研究所;
【基金】：江苏省普通高校学术研究生科研创新计划项目(KYZZ16_0495) 江苏省农业科技自主创新资金项目(CX(16)1003) 江苏省苏北科技专项(BN2016078,BN2016095)
【分类号】：S828.5

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