猪传染性胃肠炎病毒实时荧光定量PCR检测方法的建立及应用
发布时间：2018-11-20 08:13
【摘要】：针对猪传染性胃肠炎病毒(TGEV)S基因的保守序列设计引物,以TEGV疫苗毒株为模板,克隆S基因,并构建重组阳性质粒,优化反应体系和扩增条件,建立基于SYBR GreenⅠ染料的实时荧光定量PCR检测方法,并对其特异性、重复性和灵敏度进行分析。结果显示:建立的检测方法灵敏度可达10拷贝/μL,标准曲线线性关系良好(r=0.997),扩增效率高,具有良好的特异性和重复性。使用该方法对124份临床疑似TGEV病料进行检测,阳性样本有17份,检测样本的阳性率为13.7%。该方法可用于兽医临床上TGEV的快速检测和流行病学分析。
[Abstract]:According to the conserved sequence of (TGEV) S gene of porcine transmissible gastroenteritis virus, the S gene was cloned by using TEGV vaccine strain as template, and the recombinant positive plasmid was constructed to optimize the reaction system and amplification conditions. A real-time fluorescent quantitative PCR method based on SYBR Green 鈪，
本文编号：2344360
[Abstract]:According to the conserved sequence of (TGEV) S gene of porcine transmissible gastroenteritis virus, the S gene was cloned by using TEGV vaccine strain as template, and the recombinant positive plasmid was constructed to optimize the reaction system and amplification conditions. A real-time fluorescent quantitative PCR method based on SYBR Green 鈪，
本文编号：2344360
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