江苏地区2株山羊痘病毒的分离与鉴定

发布时间：2018-11-20 10:01

【摘要】：为对江苏部分地区羊场羊痘病毒进行有效的监测和防控,及时了解病毒遗传进化趋势,降低规模化养羊业的风险,本研究从江苏部分地区养羊场采集疑似羊痘病羊的皮肤丘疹、水疱或脓疱组织病料样品,分别接种9日龄SPF鸡胚绒毛尿囊膜、胎羊睾丸细胞(LT)和牛肾细胞(MDBK),观察鸡胚病变和细胞病变情况。同时通过PCR方法扩增羊痘病毒p32基因进行序列分析,并进行p32基因限制性片段长度多态性分析。结果显示,接种鸡胚绒毛尿囊膜形成典型的痘斑,胚体出血;系统进化分析显示,两株分离株属于山羊痘病毒(GTPV);限制性片段长度多态性分析结果也显示符合GTPV的特征。本研究所分离到的2株GTPV,为当地制定山羊痘的防控策略提供依据。
[Abstract]:In order to effectively monitor and control sheep pox virus in sheep farms in some parts of Jiangsu province, to understand the genetic evolution trend of the virus and to reduce the risk of large-scale sheep farming, the skin papules of sheep with suspected sheep pox disease were collected from sheep farms in some parts of Jiangsu Province. The samples of blister or pustular tissue were inoculated with the chorioallantoic membrane of 9 day old SPF chicken embryo respectively. (LT) of fetal sheep testis and (MDBK), of bovine kidney cell were used to observe the pathological changes of chicken embryo and cytopathic changes. The p32 gene of sheep pox virus was amplified by PCR and the restriction fragment length polymorphism of p32 gene was analyzed. The results showed that the chorioallantoic membrane of chorioallantoic chorioviruses inoculated with chorioallantoic membrane formed typical pockle spots and hemorrhage of embryo body. Phylogenetic analysis showed that the two isolates belonged to the restriction fragment length polymorphism of (GTPV); of goat pox virus. The two strains of GTPV, isolated in this study provide evidence for the formulation of local control strategies for goat pox.
【作者单位】：扬州大学兽医学院;江苏省动物重要疫病与人兽共患防控协同创新中心;徐州睢宁魏集镇双惠养羊专业合作社;
【基金】：苏北科技专项资金(富民强县)项目(BN2014002)
【分类号】：S852.654

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