猪圆环病毒2型山西株全基因组序列分析

发布时间：2018-11-23 07:16

【摘要】：为了解山西省猪圆环病毒2型(PCV2)的遗传变异情况,本试验采用PCR方法对山西省分离的4株猪圆环病毒流行株(SXXZ1株、SXXZ2株、SXTY株和SXJZ株)的全基因组进行了扩增、克隆和测序,并将其全基因组序列与国内外34株主要流行毒株进行核苷酸同源性与系统进化树分析。结果显示,4株PCV2山西流行株全基因组核酸序列全长SXJZ株为1 768bp,其余均为1 767bp,分别占25%和75%。4株毒株核苷酸同源性为95.9%~100.0%,与国内外34株参考株同源性为94.5%~99.9%,与国产疫苗株同源性为95.6%~99.8%;PCV2全基因组序列进化分析表明,本研究分离的SXXZ1株、SXXZ2株和SXTY株属于PCV-2b基因型,SXJZ株属于PCV-2e基因型,其中SXXZ1株和SXXZ2株与广东QY株的进化关系最近,SXTY与奥地利AUT5株的进化关系最近,SXJZ与福建FJ株的进化关系最近;而SXXZ1株、SXXZ2株和SXTY株与山西PCV2疫苗DBN-SX07-2株的进化关系较近,SXJZ株与国内PCV2疫苗LG株的进化关系较近。从而证实在山西省流行的PCV2毒株以PCV-2b为主,同时还分离出了PCV-2e亚型,表明山西省存在PCV-2e亚型毒株。本试验结果为山西省PCV2的分子流行病学、遗传变异及防控奠定了基础。
[Abstract]:In order to understand the genetic variation of porcine circovirus type 2 (PCV2) in Shanxi Province, the whole genome of four porcine circovirus epidemic strains (SXXZ1, SXXZ2, SXTY and SXJZ) were amplified by PCR method. The nucleotide homology and phylogenetic tree analysis were carried out by cloning and sequencing the whole genome sequence with 34 major epidemic strains at home and abroad. The results showed that the whole genome nucleic acid sequence of 4 strains of PCV2 in Shanxi province was 1 768 BP, while the rest was 1 767 BP, accounting for 25% and 75. 4% of the total nucleotide homology of 5. 59% and 75. 4%, respectively, and the nucleotide homology was 95. 9% and 10. 0%, respectively. The homology was 94.5% with 34 reference strains at home and abroad, 99.9 with domestic vaccine strains, and 99.8% with domestic vaccine strains. The phylogenetic analysis of PCV2 genome showed that the isolated SXXZ1 strains, SXXZ2 strains and SXTY strains belonged to PCV-2b genotypes, SXJZ strains belonged to PCV-2e genotypes, and SXXZ1 and SXXZ2 strains had the closest evolutionary relationship with QY strains in Guangdong. The evolutionary relationship between SXTY and Austrian AUT5 strain was the most recent, and that between SXJZ and Fujian FJ strain was the most recent. However, the evolutionary relationship among SXXZ1, SXXZ2 and SXTY strains was close to Shanxi PCV2 vaccine DBN-SX07-2 strain, and that of SXJZ strain was close to that of domestic PCV2 vaccine LG strain. It was confirmed that PCV-2b was the main PCV2 strain in Shanxi province, and PCV-2e subtype was also isolated, which indicated that PCV-2e subtype existed in Shanxi province. The results laid a foundation for molecular epidemiology, genetic variation and prevention and control of PCV2 in Shanxi Province.
【作者单位】：山西省农业科学院畜牧兽医研究所;陵川县畜牧兽医局;
【基金】：山西省科技攻关项目(20130311026-4)
【分类号】：S852.651

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