高水平母源抗体仔猪接种猪繁殖与呼吸综合征弱毒活疫苗的免疫效果分析

发布时间：2018-11-23 09:43

【摘要】：为评估猪繁殖与呼吸综合征(PRRS)弱毒活疫苗的免疫效果,本研究于规模猪场选取具有母源抗体的仔猪,接种PRRS弱毒活疫苗后不同时间采集血清,应用RT-PCR检测PRRS病毒(PRRSV)核酸,应用N-ELISA和G-ELISA两种试剂盒检测PRRSV抗体。结果显示,在免疫接种后60 d内可在实验仔猪血清中检测到疫苗株核酸,而在整个实验期间未能检测到野毒株核酸。仔猪在免疫前具有较高的母源抗体,阳性率达到100%,此后逐渐下降,在免疫后第28 d/60 d(第2次试验/第1次试验)降到低点后又稳步升高,从免疫后第60 d/90 d到150 d试验结束时阳性率均达到100%。应用两种ELISA试剂盒同时检测329份血清样品,检测结果的符合率为96.96%;配对χ~2检验,p0.05,两者差异不显著;Kappa值为0.81,属于极强一致性;两者相关系数r为0.794,具有显著线性相关。本研究结果表明,高水平母源抗体猪群接种弱毒活疫苗后取得良好的免疫效果,可选用N-ELISA或G-ELISA试剂盒进行免疫效果评估。
[Abstract]:In order to evaluate the immune effect of porcine reproductive and respiratory syndrome (RRS) (PRRS) attenuated live vaccine, piglets with maternal antibodies were selected from large scale pig farms and sera were collected at different times after inoculation of PRRS attenuated live vaccine. PRRS virus (PRRSV) nucleic acid was detected by RT-PCR and PRRSV antibody was detected by N-ELISA and G-ELISA kits. The results showed that the nucleic acid of the vaccine strain could be detected in the serum of the experimental piglets within 60 days after immunization, but the nucleic acid of the wild strain could not be detected during the whole experiment period. Piglets had high maternal antibody before immunization, the positive rate reached 100, then decreased gradually, and then increased steadily after 28d / 60d (test 2 / test 1). From the 60th day to the 150th day after immunization, the positive rate was 100%. Two kinds of ELISA kits were used to simultaneously detect 329 serum samples. The coincidence rate of the test results was 96.966.The paired 蠂 ~ 2 test showed that there was no significant difference between them, the Kappa value was 0.81which was very consistent. The correlation coefficient r was 0.794, and there was a significant linear correlation between them. The results showed that high level maternal antibody could be used to evaluate the immune response of pigs after inoculation of live attenuated vaccine. N-ELISA or G-ELISA kit could be used to evaluate the immune effect.
【作者单位】：广西动物疫病预防控制中心;广西大学动物科学技术学院;广西黔江示范牧场;
【基金】：广西科学研究与技术开发计划项目(桂科转14125004-22) 广西水产畜牧科技项目(桂渔牧科201528017)
【分类号】：S858.28

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