中国广西龙虎山野生猕猴粪便的病毒宏基因组学分析

发布时间：2018-11-24 09:34

【摘要】：非人灵长类携带的病毒种类繁多,其中部分对人具有致病性。为深入了解我国野生猕猴携带病毒的状况,本研究应用MiSeq高通量测序及生物信息学分析技术,对从广西采集的280份猕猴粪便标本进行了病毒宏基因组学的分析。高通量测序共获得了233 726 79条读长(Reads),其中4641条序列与病毒相关,进一步注释到27个病毒科(包含细小病毒科中的细小病毒亚科和浓核病毒亚科),包括其中5种脊椎动物病毒(占78.2%)、6种昆虫病毒(占5.5%)、11种植物病毒(占10.4%)、其他的病毒(占9.8%);遗传进化分析结果显示,被注释为萨佩罗病毒、肠道病毒、细小病毒、腺相关病毒等序列与已知病毒相似,部分序列呈现明显的差异;应用PCR扩增进一步证实了病毒序列的真实存在。本研究初步确定了广西地区猕猴粪便中病毒的病毒谱,为深入分析和研究其中有潜在公共卫生意义的病毒奠定了基础。
[Abstract]:Non-human primates carry a wide variety of viruses, some of which are pathogenic to humans. In order to understand the status of wild rhesus monkey carrying virus in China, MiSeq high-throughput sequencing and bioinformatics analysis techniques were used to analyze the viral macrogenomics of 280 fecal samples of rhesus monkeys collected from Guangxi. A total of 233,726,79 (Reads), were obtained by high-throughput sequencing, 4641 of which were associated with virus, and were further annotated to 27 families (including parvovirus subfamily and concentrated nucleovirus subfamily). There were 5 vertebrate viruses (78.2%), 6 insect viruses (5.5%), 11 plant viruses (10.4%), and other viruses (9.8%), including 5 vertebrate viruses (78.2%), 6 insect viruses (5.5%), 11 plant viruses (10.4%) and others (9.8%). The results of genetic evolution analysis showed that the sequences of SAV, enterovirus, parvovirus and adeno-associated virus were similar to those of known viruses, and some of the sequences showed obvious differences. PCR amplification further confirmed the real existence of the virus sequence. In this study, the viral spectrum of rhesus monkey feces in Guangxi was preliminarily determined, which laid a foundation for further analysis and study of the viruses with potential public health significance.
【作者单位】：湖南师范大学生命科学学院蛋白质化学与鱼类发育生物学重点实验室;中国疾病预防控制中心病毒病预防控制所病毒性腹泻室;
【基金】：国家自然科学基金(81290345) 湖南省科技计划资助项目(2014FJ3084) 湖南省教育厅资助科研项目(13C523)
【分类号】：S852.65

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