镰刀菌毒素对断奶仔猪生产性能、肠道损伤及促炎性因子的影响
发布时间：2018-12-07 15:29
【摘要】：本试验旨在探讨镰刀菌毒素对断奶仔猪生产性能、肠道二糖酶活性、氧化应激以及肠道促炎性因子的分布和mRNA表达量的影响。试验分两个阶段进行。第一阶段选择35日龄体重为8.45±0.94 kg的健康三元杂交(杜×长×大)雌性仔猪50头,仔猪随机分为2个处理,对照组20头,镰刀菌毒素组30头。对照组饲喂基础饲粮,镰刀菌毒素组饲喂含有镰刀菌毒素(玉米赤霉烯酮0.90 mg/kg,呕吐毒素1.43 mg/kg,烟曲霉毒素5.85 mg/kg)的试验饲粮,预试期7 d,正试期35 d,试验结束每个处理选取10头仔猪进行屠宰取样。结果表明:镰刀菌毒素显著降低了(P0.05)十二指肠和空肠的乳糖酶活性、空肠和回肠的蔗糖酶活性、结肠的乳糖酶和蔗糖酶活性、空肠和回肠的过氧化氢酶活力以及十二指肠、回肠和结肠的总超氧化物歧化酶和谷胱甘肽过氧化物酶活力,而空肠和回肠的丙二醛含量以及十二指肠IL-1β、十二指肠和空肠的IL-6、结肠IL-1β和IL-6的mRNA显著高于对照组(P0.05),且小肠和结肠促炎性因子的mRNA表达量存在正相关关系(P0.05);与对照组相比,镰刀菌毒素组小肠各段促炎性因子IL-1β和IL-6的阳性染色从分散在固有层细胞上向绒毛淋巴细胞胞质集中,而结肠的IL-1β和IL-6阳性点主要分布在固有层细胞胞质上。第二阶段以第一阶段屠宰试验后剩余的30头仔猪为试验动物,对照组剩余10头继续饲喂基础饲粮;镰刀菌毒素组剩余20头仔猪随机分为两个处理组,10头继续饲喂毒素饲粮作为镰刀菌毒素组,10头改饲基础饲粮作为恢复组;试验期21 d,结束后进行屠宰取样。结果表明:与对照组相比,镰刀菌毒素显著降低了(P0.05)小肠二糖酶的活性、十二指肠的总超氧化物歧化酶和谷胱甘肽过氧化物酶活力以及空肠和回肠的抗氧化酶活力,而小肠各肠段丙二醛含量显著高于对照组(P0.05);与镰刀菌毒素组相比,恢复组十二指肠的蔗糖酶活性、空肠和回肠的蔗糖酶和麦芽糖酶活性、十二指肠和空肠的总超氧化物歧化酶活力、十二指肠和回肠的谷胱甘肽过氧化物酶活力、空肠和回肠的过氧化氢酶活力显著升高(P0.05),小肠各肠段丙二醛含量有显著的降低(P0.05)。整个试验期镰刀菌毒素显著降低了(P0.05)仔猪的平均日采食量和日增重,而料重比则显著高于对照组(P0.05);与镰刀菌毒素组相比,恢复组的平均日增重和料重比有明显的改善(P0.05)。以上结果表明,本试验条件下,镰刀菌毒素能够通过氧化应激途径对肠道造成损伤并激发肠道的炎症反应,最终对断奶仔猪生产性能产生负面影响。停饲镰刀菌毒素饲粮21d后断奶仔猪的生产性能和肠道损伤有明显恢复,说明仔猪肠道能通过自身免疫修复改善生长情况。
[Abstract]:The purpose of this study was to investigate the effects of Fusarium toxin on production performance, intestinal disaccharide enzyme activity, oxidative stress, distribution of intestinal pro-inflammatory factors and expression of mRNA in weaned piglets. The experiment was conducted in two stages. In the first stage, 50 female piglets with body weight of 8.45 卤0.94 kg were randomly divided into two groups: control group (n = 20) and Fusarium toxin group (n = 30). The control group was fed with basic diet, and the Fusarium toxin group was fed with Fusarium toxin (0.90 mg/kg, vomiting toxin 1.43 mg/kg, tobacco aspergillus toxin 5.85 mg/kg) for 7 days. At the end of the trial, 10 piglets were slaughtered and sampled. The results showed that the activities of lactase in duodenum and jejunum, sucrase activity in jejunum and ileum, lactase and sucrase activity in colon were significantly decreased by Fusarium toxin. The activity of catalase in jejunum and ileum, the activity of total superoxide dismutase and glutathione peroxidase in duodenum, ileum and colon, the content of malondialdehyde in jejunum and ileum and IL-1 尾 in duodenum. The mRNA of IL-6, colon IL-1 尾 and IL-6 in duodenum and jejunum were significantly higher than those in control group (P0.05), and there was a positive correlation between mRNA expression of small intestine and colon proinflammatory factor (P0.05). Compared with the control group, the positive staining of proinflammatory factors IL-1 尾 and IL-6 in the small intestinal segments of the Fusarium toxin group was concentrated from the cells scattered in the lamina propria to the cytoplasm of the chorionic lymphocytes. The positive spots of IL-1 尾 and IL-6 in colon were mainly located in the cytoplasm of lamina propria. In the second stage, the remaining 30 piglets after the first stage slaughter trial were used as experimental animals, while the remaining 10 piglets in the control group continued to be fed with basic diet. The remaining 20 piglets in Fusarium toxin group were randomly divided into two groups: 10 piglets were fed with toxin as Fusarium toxin diet, 10 piglets were fed basal diet as recovery group, and slaughtered samples were taken after 21 days. The results showed that compared with the control group, Fusarium toxin significantly decreased the activity of small intestinal dismutase, total superoxide dismutase (SOD) and glutathione peroxidase (Glutathione peroxidase) in duodenum, and antioxidant enzyme activity in jejunum and ileum. The content of malondialdehyde in small intestine was significantly higher than that in control group (P0.05). Compared with Fusarium toxin group, sucrase activity in duodenum, sucrase and maltase activity in jejunum and ileum, total superoxide dismutase activity in duodenum and jejunum in recovery group, and total superoxide dismutase activity in duodenum and jejunum. Glutathione peroxidase activity in duodenum and ileum, catalase activity in jejunum and ileum increased significantly (P0.05), and malondialdehyde (MDA) content in intestine decreased significantly (P0.05). The average daily feed intake and daily gain of piglets were significantly decreased (P0.05), while the feed to weight ratio was significantly higher than that of the control group (P0.05). Compared with the Fusarium toxin group, the average daily gain and feed weight ratio of the recovery group were significantly improved (P0.05). The above results indicated that Fusarium toxin could damage the intestine and stimulate the inflammatory reaction of the intestine through the oxidative stress pathway under the condition of this experiment and finally had a negative effect on the production performance of weaning piglets. The production performance and intestinal injury of weaned piglets recovered obviously after 21 days of discontinuation of Fusarium toxin diet, which indicated that intestinal tract of piglets could improve growth through autoimmune repair.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28
[Abstract]:The purpose of this study was to investigate the effects of Fusarium toxin on production performance, intestinal disaccharide enzyme activity, oxidative stress, distribution of intestinal pro-inflammatory factors and expression of mRNA in weaned piglets. The experiment was conducted in two stages. In the first stage, 50 female piglets with body weight of 8.45 卤0.94 kg were randomly divided into two groups: control group (n = 20) and Fusarium toxin group (n = 30). The control group was fed with basic diet, and the Fusarium toxin group was fed with Fusarium toxin (0.90 mg/kg, vomiting toxin 1.43 mg/kg, tobacco aspergillus toxin 5.85 mg/kg) for 7 days. At the end of the trial, 10 piglets were slaughtered and sampled. The results showed that the activities of lactase in duodenum and jejunum, sucrase activity in jejunum and ileum, lactase and sucrase activity in colon were significantly decreased by Fusarium toxin. The activity of catalase in jejunum and ileum, the activity of total superoxide dismutase and glutathione peroxidase in duodenum, ileum and colon, the content of malondialdehyde in jejunum and ileum and IL-1 尾 in duodenum. The mRNA of IL-6, colon IL-1 尾 and IL-6 in duodenum and jejunum were significantly higher than those in control group (P0.05), and there was a positive correlation between mRNA expression of small intestine and colon proinflammatory factor (P0.05). Compared with the control group, the positive staining of proinflammatory factors IL-1 尾 and IL-6 in the small intestinal segments of the Fusarium toxin group was concentrated from the cells scattered in the lamina propria to the cytoplasm of the chorionic lymphocytes. The positive spots of IL-1 尾 and IL-6 in colon were mainly located in the cytoplasm of lamina propria. In the second stage, the remaining 30 piglets after the first stage slaughter trial were used as experimental animals, while the remaining 10 piglets in the control group continued to be fed with basic diet. The remaining 20 piglets in Fusarium toxin group were randomly divided into two groups: 10 piglets were fed with toxin as Fusarium toxin diet, 10 piglets were fed basal diet as recovery group, and slaughtered samples were taken after 21 days. The results showed that compared with the control group, Fusarium toxin significantly decreased the activity of small intestinal dismutase, total superoxide dismutase (SOD) and glutathione peroxidase (Glutathione peroxidase) in duodenum, and antioxidant enzyme activity in jejunum and ileum. The content of malondialdehyde in small intestine was significantly higher than that in control group (P0.05). Compared with Fusarium toxin group, sucrase activity in duodenum, sucrase and maltase activity in jejunum and ileum, total superoxide dismutase activity in duodenum and jejunum in recovery group, and total superoxide dismutase activity in duodenum and jejunum. Glutathione peroxidase activity in duodenum and ileum, catalase activity in jejunum and ileum increased significantly (P0.05), and malondialdehyde (MDA) content in intestine decreased significantly (P0.05). The average daily feed intake and daily gain of piglets were significantly decreased (P0.05), while the feed to weight ratio was significantly higher than that of the control group (P0.05). Compared with the Fusarium toxin group, the average daily gain and feed weight ratio of the recovery group were significantly improved (P0.05). The above results indicated that Fusarium toxin could damage the intestine and stimulate the inflammatory reaction of the intestine through the oxidative stress pathway under the condition of this experiment and finally had a negative effect on the production performance of weaning piglets. The production performance and intestinal injury of weaned piglets recovered obviously after 21 days of discontinuation of Fusarium toxin diet, which indicated that intestinal tract of piglets could improve growth through autoimmune repair.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28
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