新乡市鸡白痢沙门氏菌敏感药物调查分析
发布时间：2018-12-08 18:16
【摘要】：鸡白痢是由鸡沙门氏菌引起的一种发生极为普遍、危害极为严重的传染病。也是影响鸡养殖业健康发展的重要疾病之一,发生鸡白痢病造成的经济损失远大于新城疫,禽流感等重大动物疫病。因此,我们对新乡地区的鸡白痢沙门氏菌进行了流行病学调查、分离培养和生化鉴定、敏感药物筛选以及联合用药等试验,旨在研制出对新乡地区鸡白痢沙门氏菌敏感的复方药物,从而为鸡白痢沙门氏菌的防控提供一味良药。1.新乡地区鸡白痢沙门氏菌病流行病学调查为了 了解新乡地区鸡白痢沙门氏菌的流行情况,我们采用了常规的流行病学调查方法和沙门氏菌全血玻片凝集法对新乡地区40个养殖场的400份血液进行了检测。结果发现,新乡地区个别鸡场存在沙门氏菌感染的情况,且从400份采集血样中检出58份疑似沙门氏菌感染血液,发病率大约为15%,鸡沙门氏菌病呈零星分布。2.鸡白痢沙门氏菌的分离培养和鉴定为了确定疑似病鸡是否为沙门氏菌中鸡白痢沙门氏菌,我们对疑似的58只进行了病料采集、细菌分离培养、生化鉴定和血清学鉴定。最后从58份沙门氏菌病料中分离鉴定出22份鸡白痢沙门氏菌菌株。3.鸡白痢沙门氏菌敏感性药物筛选为了能够筛选出对新乡地区鸡白痢沙门氏菌敏感的药物,本试验采用药敏纸片扩散法对22株沙门氏菌进行了敏感药物筛选。通过筛选发现:新乡地区的鸡白痢沙门氏菌对氟喹诺酮类药物和多肽类药物中的硫酸粘杆菌素为中度敏感,而对链霉素、阿莫西林、氟苯尼考、乙酰甲喹等药物低度敏感或不敏感。4.新乡地区鸡白痢沙门氏菌联合用药措施为了防控新乡地区鸡白痢沙门氏菌病。本试验将对新乡地区鸡白痢沙门氏菌敏感的氟喹诺酮类药物中的恩诺沙星和多肽类中的硫酸粘杆菌素进来复方配制,并通过对鸡白痢沙门氏菌体外和体内杀菌试验,我们发现复方恩诺沙星-硫酸粘杆菌素比单独使用恩诺沙星或硫酸粘杆菌的体外杀菌试验提高了 8倍或16倍;在体内试验上提高了30%或 40%
[Abstract]:White dysentery is an infectious disease caused by salmonella. It is also one of the important diseases that affect the healthy development of chicken breeding. The economic loss caused by white dysentery is much greater than that caused by Newcastle disease, avian flu and other major animal diseases. Therefore, we conducted epidemiological investigation, isolation, culture and biochemical identification, screening of sensitive drugs and combined use of drugs for salmonella pullorum in Xinxiang area. The aim is to develop a compound drug sensitive to Salmonella pullorum in Xinxiang area, so as to provide a good medicine for the prevention and control of Salmonella pullorum. Epidemiological investigation of Salmonella pullorum in chickens in Xinxiang area in order to understand the prevalence of Salmonella pullorum in Xinxiang area, We used routine epidemiological investigation method and salmonella whole blood slide agglutination method to detect 400 blood samples from 40 farms in Xinxiang area. The results showed that there was salmonella infection in individual chicken farms in Xinxiang area, and 58 samples of suspected Salmonella infection were detected from 400 blood samples. The incidence of Salmonella infection was about 15. Isolation, Culture and Identification of Salmonella pullorum in order to determine whether the suspected infected chicken is Salmonella pullorum, 58 suspected Salmonella pullorum were collected, isolated, and identified by biochemical and serological methods. Finally, 22 strains of Salmonella pullorum were isolated from 58 samples of Salmonella pullorum. Screening of Salmonella pullorum sensitive drugs in order to screen the sensitive drugs to Salmonella pullorum in Xinxiang area 22 strains of Salmonella pullorum were screened by drug sensitive disk diffusion method. It was found that Salmonella pullorum in Xinxiang area was moderately sensitive to myxomycin sulfate in fluoroquinolones and polypeptide drugs, but to streptomycin, amoxicillin and florfenicol. Acetylmethaquine and other drugs are low sensitivity or insensitive. 4. Combined use of Salmonella pullorum in Xinxiang area to prevent and control Salmonella pullorum in Xinxiang area. In this experiment, enrofloxacin in salmonella pullorum sensitive drugs and mucin sulfate in polypeptides were prepared in Xinxiang area, and the bactericidal test of Salmonella pullorum in vitro and in vivo was carried out. We found that the in vitro bactericidal test of compound enrofloxacin-myxobacterium sulfate was 8 or 16 times higher than that of enrofloxacin or myxobacterium sulfate alone. Increased by 30% or 40% in vivo.
【学位授予单位】：南京农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.31
本文编号：2368789
[Abstract]:White dysentery is an infectious disease caused by salmonella. It is also one of the important diseases that affect the healthy development of chicken breeding. The economic loss caused by white dysentery is much greater than that caused by Newcastle disease, avian flu and other major animal diseases. Therefore, we conducted epidemiological investigation, isolation, culture and biochemical identification, screening of sensitive drugs and combined use of drugs for salmonella pullorum in Xinxiang area. The aim is to develop a compound drug sensitive to Salmonella pullorum in Xinxiang area, so as to provide a good medicine for the prevention and control of Salmonella pullorum. Epidemiological investigation of Salmonella pullorum in chickens in Xinxiang area in order to understand the prevalence of Salmonella pullorum in Xinxiang area, We used routine epidemiological investigation method and salmonella whole blood slide agglutination method to detect 400 blood samples from 40 farms in Xinxiang area. The results showed that there was salmonella infection in individual chicken farms in Xinxiang area, and 58 samples of suspected Salmonella infection were detected from 400 blood samples. The incidence of Salmonella infection was about 15. Isolation, Culture and Identification of Salmonella pullorum in order to determine whether the suspected infected chicken is Salmonella pullorum, 58 suspected Salmonella pullorum were collected, isolated, and identified by biochemical and serological methods. Finally, 22 strains of Salmonella pullorum were isolated from 58 samples of Salmonella pullorum. Screening of Salmonella pullorum sensitive drugs in order to screen the sensitive drugs to Salmonella pullorum in Xinxiang area 22 strains of Salmonella pullorum were screened by drug sensitive disk diffusion method. It was found that Salmonella pullorum in Xinxiang area was moderately sensitive to myxomycin sulfate in fluoroquinolones and polypeptide drugs, but to streptomycin, amoxicillin and florfenicol. Acetylmethaquine and other drugs are low sensitivity or insensitive. 4. Combined use of Salmonella pullorum in Xinxiang area to prevent and control Salmonella pullorum in Xinxiang area. In this experiment, enrofloxacin in salmonella pullorum sensitive drugs and mucin sulfate in polypeptides were prepared in Xinxiang area, and the bactericidal test of Salmonella pullorum in vitro and in vivo was carried out. We found that the in vitro bactericidal test of compound enrofloxacin-myxobacterium sulfate was 8 or 16 times higher than that of enrofloxacin or myxobacterium sulfate alone. Increased by 30% or 40% in vivo.
【学位授予单位】：南京农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.31
【引证文献】
相关期刊论文 前1条
1 廖洁丹;刘敏芳;谭华龙;陈建红;张济培;;竹丝鸡鸡白痢沙门菌的分离鉴定与药敏试验[J];中国家禽;2017年16期
，本文编号：2368789
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