6株猪源芽孢杆菌的分离鉴定及其益生性研究

发布时间：2018-12-11 06:35

【摘要】：本研究旨在通过高温处理和常规厌氧菌分离法从健康肥猪粪便中分离芽孢杆菌,并通过培养性状、生化试验、PCR、肠道耐受、抗菌活性和接种试验动物等方法鉴定筛选性状稳定、优良的益生性芽孢杆菌。结果显示,共分离到6株芽孢杆菌,其中1株巨大芽孢杆菌、1株死谷芽孢杆菌、2株解淀粉芽孢杆菌和2株枯草芽孢杆菌;分离菌对多黏菌素和青霉素耐药性较强,对其他12种常用药物较敏感,对致病性大肠杆菌和金黄色葡萄球菌的抑菌效果较好;4种芽孢杆菌均可在酸性、高胆盐和高温条件下生存,特别是巨大芽孢杆菌和枯草芽孢杆菌耐受能力较强;分离的芽孢杆菌对小鼠均无致病性,其中巨大芽孢杆菌和死谷芽孢杆菌有较明显的促进试验动物生长的作用。结果提示,巨大芽孢杆菌和死谷芽孢杆菌可作为益生性芽孢杆菌的初步选择。
[Abstract]:The purpose of this study was to isolate Bacillus spp from the feces of healthy pigs by means of high temperature treatment and routine anaerobic isolation. The purpose of this study was to isolate Bacillus spp from feces of healthy pigs. The purpose of this study was to isolate bacillus from feces of healthy Antimicrobial activity and inoculation of experimental animals were used to identify the stable and excellent probiotic Bacillus. The results showed that six strains of Bacillus were isolated, one of them was Bacillus megaterium, one was Bacillus solani, two were Bacillus amylolyticus and two were Bacillus subtilis. The isolates were resistant to polymyxin and penicillin, sensitive to other 12 common drugs, and had better bacteriostatic effect on pathogenic Escherichia coli and Staphylococcus aureus. All the four Bacillus species could survive in acid, high bile salt and high temperature, especially Bacillus megaterium and Bacillus subtilis. The isolated bacillus had no pathogenicity to mice, among which Bacillus megaterium and Bacillus cereus had obvious effects on the growth of experimental animals. The results suggest that Bacillus megaculis and Bacillus stillus can be used as the primary selection of probiotic Bacillus.
【作者单位】：江西农业大学动物科学与技术学院;南昌金牧动物保健服务有限公司;
【基金】：江西省科技支撑计划(20132BBF60038) 江西省生猪产业体系(JXARS-03-疫病防控岗位)
【分类号】：S852.61

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