发酵与酶解处理玉米秸秆工艺优化及其对饲用品质影响研究
发布时间：2018-12-11 22:51
【摘要】：本文研究了发酵和酶解对玉米秸秆的影响并优化得到最佳工艺参数,以粗蛋白含量、粗纤维降解率、NDF降解率、ADF降解率、半纤维素降解率、纤维素降解率、木质素降解率、总酸含量、总糖和还原糖含量为考察指标,研究比较多菌发酵、单独酶解以及菌酶结合处理对玉米秸秆饲用品质的改善情况。以玉米秸秆粗蛋白含量为评价指标,通过单因素试验考察了发酵时间、发酵温度、酵母和乳酸菌接种量对玉米秸秆发酵后粗蛋白含量变化的影响,并在单因素基础上通过响应面试验优化了玉米秸秆发酵工艺参数,确定了玉米秸秆最佳发酵工艺:即在发酵时间为9天,发酵温度为40℃、接种量为1.5%,在此发酵条件下玉米秸秆的粗蛋白含量达到了15.47%左右。以玉米秸秆粗纤维降解率为评价指标,通过单因素试验考察了pH、酶解温度、酶解时间和酶浓度对玉米秸秆酶解后粗纤维变化的影响,并在单因素基础上通过响应面试验优化了玉米秸秆酶解工艺参数,确定了玉米秸秆最佳发酶解工艺:即pH为5.0、酶解温度为55℃、酶解时间60h,酶浓度为50 FPU/g,在此酶解条件下,玉米秸秆粗纤维降解率达到了45.62%左右。以玉米秸秆粗蛋白含量、粗纤维降解率、总酸含量、总糖和还原糖含量为指标,考察了发酵和酶解共同处理对玉米秸秆饲粮化品质的影响。结果表明,当纤维素酶和菌类发酵剂共同处理玉米秸秆时,其粗蛋白含量比对照组提高了近四倍,并且比单独酶解作用高13%,比单独发酵提高了5%。玉米秸秆经过酶解与发酵共同处理后粗纤维降解更加显著,是对照组的5.5倍,降解效果均优于单独酶解和单独菌类发酵。菌酶共同处理后NDF和ADF降解率分别为25.57±2.67%和21.29±1.37%,降解效果均优于单独酶解和单独菌类发酵。菌酶共同处理后半纤维素降解率高达50.61±6.67%,降解效果明显优于单独处理。菌酶共同处理后纤维素降解率是对照组的近6倍,达到13.54±2.65%,均高于双菌发酵和单独酶解的降解率。菌酶共同处理后木质素降解率达到49.56±6.65%,降解的能力明显高于双菌发酵和单独酶解。经过酵母和乳酸菌发酵后,玉米秸秆中总酸含量迅速上升了近一倍,经过酶解处理的玉米秸秆的总酸含量也比对照组高出约0.3%左右,而菌酶结合处理后的玉米秸秆的产酸量明显高于单独发酵和单独酶解作用,与对照组相比提高了一倍。发酵和酶解共同处理玉米秸秆后,总糖和还原糖含量略有降低,这可能是与乳酸菌利用糖类生长繁殖有关。
[Abstract]:In this paper, the effects of fermentation and enzymatic hydrolysis on corn straw were studied and the optimum technological parameters were obtained, including crude protein content, crude fiber degradation rate, NDF degradation rate, ADF degradation rate, hemicellulose degradation rate, lignin degradation rate. The contents of total acid, total sugar and reducing sugar were used as indexes to study the improvement of forage quality of corn straw by multi-bacterial fermentation, single enzymatic hydrolysis and bacteria-enzyme binding treatment. The effects of fermentation time, fermentation temperature and inoculation amount of yeast and lactic acid bacteria on the content of crude protein in corn straw were investigated by single factor experiment. On the basis of single factor, the fermentation parameters of corn straw were optimized by response surface test. The optimum fermentation technology was determined: the fermentation time was 9 days, the fermentation temperature was 40 鈩，
本文编号：2373369
[Abstract]:In this paper, the effects of fermentation and enzymatic hydrolysis on corn straw were studied and the optimum technological parameters were obtained, including crude protein content, crude fiber degradation rate, NDF degradation rate, ADF degradation rate, hemicellulose degradation rate, lignin degradation rate. The contents of total acid, total sugar and reducing sugar were used as indexes to study the improvement of forage quality of corn straw by multi-bacterial fermentation, single enzymatic hydrolysis and bacteria-enzyme binding treatment. The effects of fermentation time, fermentation temperature and inoculation amount of yeast and lactic acid bacteria on the content of crude protein in corn straw were investigated by single factor experiment. On the basis of single factor, the fermentation parameters of corn straw were optimized by response surface test. The optimum fermentation technology was determined: the fermentation time was 9 days, the fermentation temperature was 40 鈩，
本文编号：2373369
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