高通量测序分析人工养殖成年林麝粪便古菌菌群多样性

发布时间：2018-12-14 08:58

【摘要】：本试验的目的是采用高通量测序技术研究人工养殖成年林麝粪便中古菌的结构和组成,并对比不同性别之间的差异。选取12只3岁健康的林麝依据性别分为雄性组(M组)和雌性组(F组),每组各6只。采集其新鲜粪便,提取DNA,用古菌通用引物PCR扩增古菌16S rRNA的V4~V5区,用M i Seq 300 PE测序平台对扩增产物进行高通量测序,用QIIM E等软件对测序序列进行分析统计。结果表明:在从门到属的各级分类水平上,F组与M组古菌的相对丰度差异均不显著(P0.05)。M组和F组的组内遗传距离分别为0.16±0.03和0.27±0.06,组间为0.24±0.07,样品的相似度很高。林麝粪便中的古菌可以分为3个门,优势门为阔古细菌门(Euryarchaeota);在属水平上可以分为7个已知属,优势属为甲烷短杆菌属(Methanobrevibacter),其次为热裸单胞菌属(Thermogymnomonas)。结果提示,雄性和雌性林麝粪便中古菌的结构和组成都没有显著差异,Methanobrevibacter是林麝粪便中的优势古菌属
[Abstract]:The purpose of this study was to study the structure and composition of archaic bacteria in the feces of adult musk deer cultured in artificial forest by using high-throughput sequencing technique and to compare the differences between sexes. Twelve healthy forest musk deer aged 3 years were divided into male group (M group) and female group (F group) according to their sex. Fresh feces were collected, DNA, was extracted and the V4~V5 region of 16s rRNA was amplified by PCR, high throughput sequencing was carried out by M i Seq 300 PE sequencing platform, and the sequence was analyzed by QIIM E and other software. The results showed that there was no significant difference in relative abundance between group F and group M at the taxonomic level from phylum to genus (P 0.05). The genetic distances in group). M and group F were 0.16 卤0.03 and 0.27 卤0.06, respectively. The similarity between the two groups was 0.24 卤0.07, and the similarity of the samples was very high. The ancient bacteria in the faeces of musk deer can be divided into 3 phylum, and the dominant phylum is (Euryarchaeota);, which can be divided into 7 known genera at the generic level, and the dominant genus is (Methanobrevibacter), followed by (Thermogymnomonas). The results showed that there was no significant difference in the structure and composition between male and female forest musk deer faeces, and Methanobrevibacter was the dominant ancient fungus in the faeces of forest musk deer.
【作者单位】：四川农业大学动物营养研究所动物抗病营养教育部重点实验室;四川养麝研究所;
【基金】：科技部国际合作项目“畜禽低碳养殖关键调控技术合作研究”(2014DFA32860)
【分类号】：S865.41;S852.6

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