猪支气管败血波氏杆菌的分离鉴定及部分生物学特性的研究

发布时间：2018-12-14 11:41

【摘要】：为了解猪支气管败血波氏杆菌(Bb)在四川规模化猪场的感染情况,为猪支气管败血波氏杆菌的诊断及临床治疗提供参考,本研究从四川省5个猪场采取具有呼吸道症状的猪鼻腔棉拭子16份,采用改良Sm ith培养基分离该菌,通过对该菌的生长特性、菌落形态和革兰染色菌体形态和排列方式进行观察以及对特异性基因fla和D nt进行PCR检测,共分离鉴定出4株Bb,该菌从猪场中的分离率为80%(4/5),猪个体阳性率为25%(4/16)。小鼠皮肤坏死试验结果表明,小鼠颈部皮下注射分离菌粗提的Dnt毒素后,注射局部皮肤坏死,剖检见脾萎缩、肺充血和肝边缘坏死。药敏试验结果表明,分离菌株对头孢唑啉、四环素、环丙沙星等14种抗生素高度敏感,对氨苄西林、杆菌肽、复方新诺明等7种抗生素耐药率较高,试验结果说明,四川省猪群中存在致病性较强的猪支气管败血波氏杆菌,为选用敏感抗菌药物预防和治疗Bb病提供了依据。
[Abstract]:In order to understand the infection of (Bb) in Sichuan large-scale swine farm, and to provide reference for the diagnosis and clinical treatment of Bacillus bronchius in swine. In this study, 16 pig nasal cotton swabs with respiratory symptoms were collected from 5 pig farms in Sichuan Province. The bacteria were isolated by modified Sm ith medium. Colony morphology and Gram staining cell morphology and arrangement were observed, and specific genes fla and D nt were detected by PCR. Four strains of Bb, were isolated and identified from pig farm. The isolation rate of Bb, from pig farm was 80% (4 / 5). The positive rate of pigs was 25% (4 / 16). The results of skin necrosis test showed that local skin necrosis, spleen atrophy, pulmonary hyperemia and liver edge necrosis were observed after subcutaneous injection of Dnt toxin extracted from isolated bacteria in the neck of mice. The results of drug sensitivity test showed that the isolated strains were highly sensitive to 14 antibiotics, such as cefazolin, tetracycline, ciprofloxacin, etc. There is a strong pathogenicity of Bordetella bronchus septicus in swine population in Sichuan Province, which provides a basis for the selection of sensitive antimicrobial agents for the prevention and treatment of Bb's disease.
【作者单位】：西南民族大学生命科学与技术学院;
【基金】：“十三五”国家重点研发计划项目(2016YFD0500705) 西南民族大学研究生“创新型科研项目”(CX2017SZ078) 兽医专业学位研究生省级教育实践基地建设项目
【分类号】：S852.61

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