宁夏地区奶牛乳房炎源沙门氏菌的分离鉴定与耐药性研究

发布时间：2018-12-15 17:22

【摘要】：乳房炎是奶牛养殖过程中最常见的一种多发性疾病,.不仅对患病奶牛的产乳量、产乳品质有很大的影响,而且严重影响奶牛的产后发情时间以及妊娠时间,还会危及人类健康。本研究利用宁夏地区所患临床型以及隐性型乳房炎的奶牛为来源,采集乳样分离疑似沙门氏菌菌株,分别进行了奶牛乳房炎源沙门氏菌的分离,生化鉴定试验,相关基因的PCR检测方法的建立与应用；奶牛乳房炎源沙门氏菌的血清型的鉴定分析；奶牛乳房炎源沙门氏菌的耐药性检测试验。1.奶牛乳房炎源沙门氏菌的分离,生化鉴定试验,相关基因的PCR(聚合酶链式反应)检测方法的建立与应用：利用传统方法,沙门氏菌鉴定培养基分离鉴定,共得到疑似分离株12株；根据相关报道,设计出五种鉴定沙门细菌常用引物hns、invA、 hilA、hut、sms,建立PCR方法,对奶牛乳房炎源沙门氏菌DNA(脱氧核糖核酸)进行PCR扩增并进行确认。最终,应用此方法鉴定出本次试验收集到的12株奶牛乳房炎源沙门氏菌疑似株中,12株均为沙门氏菌。2.奶牛乳房炎源沙门氏菌的血清型的鉴定分析：对现有的12株奶牛乳房炎源沙门氏菌进行O抗原血清学鉴定。鉴定结果表明,12株奶牛乳房炎源沙门氏菌株优势血清型为04(66.7%)、04,12(66.7%)、09,12(33.3%),以多价为主。3.奶牛乳房炎源沙门氏菌的耐药性检测试验。对12株奶牛乳房炎源沙门氏菌进行耐药性试验,发现多株沙门氏菌出现多重耐药性。耐药性高于50%有NAL(萘啶酸)、AMP(氨苄西林)、CFI(头孢唑啉)、STR(链霉素)、TET(四环素)、DC(多西环素)、AZI(阿奇霉素)一共七种,占本实验使用抗生素的58.3%,耐药率低于20%有NOR(诺氟沙星)、CRO(头孢曲松钠)、CTX(头孢噻肟)、AKN(丁胺卡那)占本实验总是用抗生素种类的33.3%。各株细菌对抗生素的耐药性存在较大差异。
[Abstract]:Mastitis is one of the most common diseases in dairy cattle breeding. It not only has a great influence on milk yield and milk quality of sick cows, but also seriously affects the time of postpartum estrus and pregnancy time of dairy cows, and also endangers human health. In this study, milk samples were collected from clinical and recessive mastitis cows in Ningxia area to isolate the suspected Salmonella strains, and the isolation and biochemical identification of Salmonella from dairy mastitis were carried out. Establishment and application of PCR detection method for related genes; Identification and analysis of serotypes of salmonella from mastitis in dairy cattle; drug resistance test of salmonella from mastitis in dairy cattle. 1. Isolation of Salmonella from Bovine mastitis, biochemical Identification Test, Establishment and Application of PCR (Polymerase chain reaction) method for Detection of related genes: isolation and Identification of Salmonella by traditional methods, A total of 12 suspected isolates were obtained. According to relevant reports, five kinds of primers hns,invA, hilA,hut,sms, were designed to establish PCR method to amplify and confirm DNA (deoxyribonucleic acid) of Salmonella mastitis. Finally, this method was used to identify 12 suspected strains of salmonella from cow mastitis collected in this experiment, 12 of which were salmonella. Identification and analysis of serotypes of Salmonella mastitis in dairy cattle: 12 strains of Salmonella mastitis were serologically identified by O antigen. The results showed that the dominant serotypes of 12 strains of salmonella from mastitis of dairy cattle were 04 (66.7%), 04 (66.7%), 09 (33.3%), respectively. Drug Resistance Test of Salmonella from mastitis in Dairy cows. The drug resistance of 12 strains of Salmonella from mastitis of dairy cattle was tested and many strains of salmonella were found to have multiple drug resistance. More than 50% of drug resistance was found in seven NAL (), AMP (nalididinate, ampicillin,), CFI (, cefazolin,), STR (, streptomycin,), TET (, tetracycline,), DC (, doxycycline,), AZI (, azithromycin). NOR (norfloxacin), CRO (ceftriaxone sodium), CTX (cefotaxime), AKN (butyrate) accounted for 33.3% of the antibiotics used in this experiment. There were significant differences in antibiotic resistance among different strains of bacteria.
【学位授予单位】：宁夏大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.23

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