宠物犬及犬粮源沙门氏菌的耐药性分析及PFGE分型

发布时间：2018-12-15 17:53

【摘要】：为了解宠物犬和犬粮源沙门氏菌的耐药表型特征与耐药基因分布,以及不同分离菌株之间的同源性,本研究采用K-B法测定27株分离菌的药物敏感性、PCR检测耐药基因、脉冲场凝胶电泳(PFGE)进行基因分型。结果显示,27株沙门氏菌分离株对氨苄西林、阿莫西林/克拉维酸、链霉素、复方新诺明、强力霉素耐药率在29.63%~22.22%,33.33%分离菌对3种及3种以上抗生素耐药,其中阿莫西林/克拉维酸-氨苄西林-复方新诺明-强力霉素所占比率最大,为66.67%(6/9);74.07%分离菌至少含有1种耐药基因,以str A-B、bla PSE、tet B、sul1检出率最高,分别为40.7%、37.0%、29.6%、25.9%,四环素类、氯霉素类、磺胺类、β-内酰胺类耐药基因与耐药表型符合率较高,分别为100%、100%、87.5%、72.7%;27株分离菌形成12种PFGE基因型,优势型别为pt1和pt7型。本研究结果表明宠物犬和犬粮源沙门氏菌呈现多重耐药,耐药表型与其相对应的耐药基因间存在密切的相关性;PFGE基因型呈多态性,而其与血清型之间不呈相关性,但与多重耐药菌株(耐8)间存在一定程度的关联性。
[Abstract]:In order to understand the phenotypic characteristics of drug resistance and the distribution of drug resistance genes in dog and dog grain source Salmonella and the homology among different isolates, K-B method was used to determine the drug sensitivity of 27 isolates and PCR was used to detect drug resistance genes. The genotyping was performed by pulsed field gel electrophoresis (PFGE). The results showed that 27 Salmonella isolates were resistant to ampicillin, amoxicillin / clavulanic acid, streptomycin, compound sulfamycin, doxycycline and doxycycline. 33. 33% of the isolates were resistant to 3 or more antibiotics, among which amoxicillin / clavulanic acid-ampicillin-doxycycline was the largest (66.67%). 74.07% of the isolates contained at least one drug resistance gene, and the detection rate of str A-Bnbla PSE,tet Bnsul1 was the highest, which was 40.7% and 37.0%, and 29.6% and 25.9%, respectively, tetracycline, chloramphenicol, sulfamide, tetracycline, chloramphenicol, sulfanilamide. The coincidence rate between 尾 -lactam resistance genes and phenotypes of drug resistance was 100%, 87.5% and 72.7%, respectively. There were 12 PFGE genotypes in 27 isolates, and the dominant genotypes were pt1 and pt7. The results showed that there were multiple drug resistance in dog and dog grain source Salmonella, and there was a close correlation between drug resistance phenotype and its corresponding drug resistance gene. PFGE genotypes were polymorphic, but not correlated with serotype, but correlated with multidrug resistant strains (8) to some extent.
【作者单位】：安徽农业大学动物科技学院;
【基金】：国家级大学生创新训练项目(201410364003) 安徽省自然科学基金项目(1508085MC44) 安徽省高等学校省级自然科学研究项目(KJ2008B056) 安徽省质量工程项目(2013sxzx008)
【分类号】：S852.61

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