宁夏某肉牛场牛支原体分离鉴定及病理组织学观察

发布时间：2018-12-16 23:07

【摘要】：为了分离鉴定宁夏某肉牛场牛支原体和分析该病原对靶器官的损伤,采用Thiaucourt's液体筛选培养基和固体培养基进行病原分离,设计牛支原体16SrRNA通用引物和uvrC特异性引物进行基因序列扩增并测序,使用DNA Star软件将分离菌株测序结果与GenBank中的标准株序列进行同源性比较,采用MEGA6.0软件中的邻接法(Neighbor-joining,NJ)依据16SrRNA和uvrC序列构建分离株系统发育树并进行遗传进化分析,采用石蜡切片,HE染色,病理组织学观察。结果表明,病原菌落呈油煎蛋样,16S rRNA和uvrC扩增片段与阳性对照一致,16S rRNA和uvrC基因序列构建的系统发育树与牛支原体在同一分支;肺组织结构消失,部分肺泡腔实变、塌陷,局灶性肺出血,肺泡腔内可见炎性细胞浸润;肺泡隔增厚、出血,肺泡内有少量脱落的上皮细胞;肺泡壁断裂,肺泡腔可见红色的炎性渗出物,有纤维结缔组织增生。
[Abstract]:In order to isolate and identify Mycoplasma bovis in a beef farm in Ningxia and analyze the damage of the pathogen to the target organ, Thiaucourt's liquid screening medium and solid medium were used to isolate the pathogen. 16SrRNA universal primers and uvrC specific primers for Mycoplasma bovis were designed to amplify the gene sequence and sequenced. The sequence of isolated strains was compared with that of standard strains in GenBank by DNA Star software. The phylogenetic tree of isolated strains was constructed by Neighbor-joining,NJ in MEGA6.0 software according to 16SrRNA and uvrC sequences, and the phylogenetic analysis was carried out. Paraffin sections, HE staining and histopathology were used to observe the phylogenetic tree. The results showed that the pathogenic bacteria were like fried eggs, the 16s rRNA and uvrC amplified fragments were consistent with the positive control, and the phylogenetic tree constructed by 16s rRNA and uvrC gene sequence was in the same branch as Mycoplasma bovis. The pulmonary tissue structure disappeared, partial alveolar cavity consolidation, collapse, focal pulmonary hemorrhage, inflammatory cell infiltration in alveolar cavity, alveolar septum thickening, hemorrhage, a small number of exfoliated epithelial cells in the alveoli. Alveolar wall rupture, alveolar cavity visible red inflammatory exudates, fibrous connective tissue proliferation.
【作者单位】：宁夏大学农学院;
【基金】：宁夏科技攻关重点专项项目(312-0164)
【分类号】：S852.62

【相似文献】