禽波氏杆菌山东地区不同分离株的致病性研究

发布时间：2018-12-18 17:31

【摘要】：为检测禽波氏杆菌鸡胚分离株和呼吸道分离株对雏鸡的致病性差异,本研究对2004年~2011年分离鉴定的12株禽波氏杆菌进行了鸡胚半数致死量和雏鸡半数致死量的检测,筛选强毒菌株进行雏鸡的鼻腔感染试验。结果表明LL、XT和AQ分离株的毒力最强,其它分离株的毒力稍弱。3株强毒菌株均可感染雏鸡呼吸道和肺脏,引起呼吸道症状以XT和AQ的症状最为明显,而在其它内脏中造成感染的鸡只比例以LL株最高,可达30%~40%;AQ最低,仅10%。HE染色结果显示对气管的损伤以XT最强,而肺脏、肝脏等内脏器官的病理变化以LL最严重。免疫酶组化试验也证实LL在内脏组织中的载量要多于XT和AQ。3株菌株均可导致雏鸡的生长抑制和免疫器官损伤,对新城疫灭活苗免疫后的抗体水平具有轻微的抑制作用,且均以LL的抑制作用最强。实验结果显示在所检测的12株禽波氏杆菌中,鸡胚分离株LL毒力最强,具有比呼吸道分离株XT和AQ更强的感染雏鸡内脏组织的能力,本研究为进一步鉴定禽波氏杆菌毒力因子和免疫原性奠定了基础。
[Abstract]:In order to detect the pathogenicity of chicken embryo isolates and respiratory tract isolates of Boxella fowls to chicks, the 50% lethal dose of chicken embryos and the 50% lethal dose of chicken chickens were determined in 12 isolates isolated from 2004 to 2011. The nasal cavity infection test of chicks was carried out by screening virulent strains. The results showed that the virulence of LL,XT and AQ isolates was the strongest, while the virulence of other isolates was weaker. All three virulent strains could infect the respiratory tract and lung of chicks, and the respiratory symptoms of XT and AQ were the most obvious. In other viscera, the proportion of chickens infected with LL strain was the highest, up to 30% and 40%. AQ was the lowest, only 10%.HE staining showed that XT was the most serious injury to trachea, while LL was the most serious in visceral organs such as lung and liver. The immunohistochemical test also confirmed that LL in visceral tissue was more than that of XT and AQ.3 strains, which could induce growth inhibition and immune organ damage in chicks, and had a slight inhibitory effect on the antibody level of Newcastle disease inactivated vaccine after immunization. The inhibitory effect of LL was the strongest. The results showed that the chicken embryo isolate (LL) was the most virulent of the 12 strains detected, and had stronger ability to infect chicken visceral tissue than respiratory isolates XT and AQ. This study laid a foundation for further identification of virulence factor and immunogenicity of B. fowls.
【作者单位】：山东农业大学动物科技学院;山东省动物疫病预防与控制中心;
【基金】：山东省自然科学基金项目(ZR2014CM044) 国家自然科学基金项目(31602067、31272595、30972183)
【分类号】：S852.61

【相似文献】