杜仲叶对绵羊脂肪代谢的影响及其机理
发布时间：2018-12-18 20:52
【摘要】：为研究杜仲叶对绵羊脂肪代谢和肉品质的影响,本研究随机选择70~80日龄、体重25~30 kg的绵羊(湖羊)30只,平均分为3组,分别为对照组(CTL组,饲粮中不含杜仲叶)、低水平杜仲叶组(EUL1组,饲粮含10%杜仲叶)和高水平杜仲叶组(EUL2组,饲粮含20%杜仲叶),每组10只。预试期15 d,正试期90 d。采集血液,测定血浆脂质代谢分析指标;采集背最长肌,测定营养物质含量;采集肝脏组织,测定脂肪代谢相关酶和核转录因子的mRNA表达水平和蛋白水平。结果表明:1)各组干物质采食量EUL1组EUL2组CTL组,组间差异显著(P0.05)。2)与CTL组相比,EUL2组血浆极低密度脂蛋白(VLDL)、高密度脂蛋白(HDL)、非酯化脂肪酸(NEFA)含量显著升高(P0.05),EUL1和EUL2组血浆低密度脂蛋白(LDL)(0.05≤P0.10)、总胆固醇(TC)(P0.05)含量降低。3)与CTL组相比,EUL1组肌肉粗蛋白质含量、EUL2组肌肉粗脂肪含量显著升高(P0.05),EUL1组肌肉剪切力显著减小(P0.05)。4)与CTL组相比,EUL1组和EUL2组肌肉饱和脂肪酸(SFA)含量略有降低(P0.05),肌肉不饱和脂肪酸(UFA)含量显著升高(P0.05),其中,EUL2组肌肉MUFA、PUFA含量及UFA/SFA显著升高(P0.05)。5)与CTL组相比,杜仲叶能够显著下调肝脏脂肪合成相关酶硬脂酰辅酶A去饱和酶1(SCD1)和核转录因子固醇调节元件结合蛋白1c(SREBP-1c)、过氧化物酶体增生激活受体γ(PPARγ)的mRNA表达水平(P0.05);显著上调肝脏脂肪酸分解相关酶肉毒碱棕榈酰基转移酶1A(CPT1A)、脂蛋白酯酶(LPL)mRNA表达水平显著上调(P0.05);与CTL组相比,EUL2组肝脏SCD1蛋白水平显著下降(P0.05)。结果提示,杜仲叶通过调节脂肪合成和分解相关基因的表达,显著影响绵羊脂肪的代谢和肌肉脂肪酸的组成。
[Abstract]:In order to study the effect of Eucommia ulmoides leaves on fat metabolism and meat quality of sheep, 30 sheep (Hu sheep) were randomly divided into three groups (CTL group, diet without Eucommia ulmoides). Low level Eucommia ulmoides leaves group (EUL1 group, diet containing 10% Eucommia ulmoides leaves) and high level Eucommia ulmoides leaf group (EUL2 group, diet containing 20% Eucommia ulmoides leaves), 10 rats in each group. The pre-test period was 15 days and the current test period was 90 days. Blood was collected and plasma lipid metabolism was measured; longissimus dorsi muscle was collected to determine the content of nutrients; liver tissue was collected to determine the mRNA expression level and protein level of lipid-metabolization-related enzymes and nuclear transcription factors. The results showed that: 1) there was significant difference between CTL group and CTL group in EUL1 group (P0.05). 2. Compared with CTL group, very low density lipoprotein (VLDL), high density lipoprotein (HDL), in EUL2 group was higher than that in CTL group. The content of nonesterified fatty acid (NEFA) increased significantly (P0.05), plasma LDL (LDL) (0.05 鈮，
本文编号：2386486
[Abstract]:In order to study the effect of Eucommia ulmoides leaves on fat metabolism and meat quality of sheep, 30 sheep (Hu sheep) were randomly divided into three groups (CTL group, diet without Eucommia ulmoides). Low level Eucommia ulmoides leaves group (EUL1 group, diet containing 10% Eucommia ulmoides leaves) and high level Eucommia ulmoides leaf group (EUL2 group, diet containing 20% Eucommia ulmoides leaves), 10 rats in each group. The pre-test period was 15 days and the current test period was 90 days. Blood was collected and plasma lipid metabolism was measured; longissimus dorsi muscle was collected to determine the content of nutrients; liver tissue was collected to determine the mRNA expression level and protein level of lipid-metabolization-related enzymes and nuclear transcription factors. The results showed that: 1) there was significant difference between CTL group and CTL group in EUL1 group (P0.05). 2. Compared with CTL group, very low density lipoprotein (VLDL), high density lipoprotein (HDL), in EUL2 group was higher than that in CTL group. The content of nonesterified fatty acid (NEFA) increased significantly (P0.05), plasma LDL (LDL) (0.05 鈮，
本文编号：2386486
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