一例肉鸡心包积水-包涵体肝炎综合征的诊断
发布时间：2018-12-20 15:32
【摘要】：采集疑似心包积水-包涵体肝炎综合征(HPS-IBH)病鸡肝脏、肺脏、脾脏、法氏囊等病料,采用针对禽腺病毒(FAdV)的Fiber基因及传染性法氏囊病病毒(IBDV)的vVP2、VP1-b基因设计特异引物进行病毒的PCR检测以及基因序列测定与分析,并对病变肝脏组织进行组织病理学观察。结果:分别在肝脏、脾脏、肺脏组织中扩增出FAdV-Fiber基因的目的条带,在法氏囊和脾脏组织中扩增出IBDV-vVP2、-VP1-b基因的目的条带;经测序分析,该病例中FAdV属于Ⅰ群禽腺病毒血清4型(FAdV-4),IBDV属于基因重排病毒;病变肝脏组织H-E染色观察显示,肝细胞核内有典型的嗜碱性包涵体。综合分析,确诊该HPS-IBH病例是由于鸡群感染IBDV后继发感染FAdV-4所致。
[Abstract]:Liver, lung, spleen and bursa were collected from chickens with suspected pericardial hydronephrosis and inclusion body hepatitis syndrome (HPS-IBH). Fiber gene of (FAdV) and vVP2, of (IBDV) of infectious bursal disease virus were used. The specific primers designed for VP1-b gene were used to detect the virus by PCR, to detect the gene sequence, and to observe the histopathology of the diseased liver tissue. Results: the target bands of FAdV-Fiber gene were amplified in liver, spleen and lung, and IBDV-vVP2,-VP1-b gene was amplified in bursa of Fabricius and spleen. Sequencing analysis showed that FAdV belonged to group 鈪，
本文编号：2388187
[Abstract]:Liver, lung, spleen and bursa were collected from chickens with suspected pericardial hydronephrosis and inclusion body hepatitis syndrome (HPS-IBH). Fiber gene of (FAdV) and vVP2, of (IBDV) of infectious bursal disease virus were used. The specific primers designed for VP1-b gene were used to detect the virus by PCR, to detect the gene sequence, and to observe the histopathology of the diseased liver tissue. Results: the target bands of FAdV-Fiber gene were amplified in liver, spleen and lung, and IBDV-vVP2,-VP1-b gene was amplified in bursa of Fabricius and spleen. Sequencing analysis showed that FAdV belonged to group 鈪，
本文编号：2388187
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