无铜饲料对小鼠肠道菌群的影响
发布时间：2018-12-21 21:22
【摘要】：铜是动物机体必需的金属元素,参与重要的代谢反应。本试验旨在研究无铜饲料对小鼠肠道微生物菌落组成和相关代谢通路的影响,选择健康、体重相近、遗传背景为野生型C57BL/6J的成年公鼠20只,随机分为2组,试验组和对照组各10只。待小鼠适应性饲养1周后用无铜饲料饲喂,对照组饲喂25 mg/L含铜水,试验组饲喂无铜水,试验期30 d。试验期结束时,尾静脉采血进行铜蓝蛋白含量的测定,并采集小鼠新鲜盲肠内容物利用高通量测序技术对小鼠的盲肠内容物16S rRNA基因组进行分析。结果显示,与对照组相比,无铜组小鼠血清铜蓝蛋白含量极显著降低(P0.001)。无铜组小鼠盲肠微生物菌群在门、属水平分别与对照组有5和43个差异显著菌群(P0.05);通过差异菌群功能预测分析发现,两组共有19种KEGG二级代谢通路差异显著(P0.05),其中与代谢相关的功能是脂代谢、甘氨酸生物合成与代谢、多酮类化合物和萜类化合物代谢、其他次生代谢产物的生物合成和氨基酸代谢,说明饲料中铜的缺失可以改变少数肠道菌群的组成及其功能,本试验为研究铜在小鼠肠道菌群结构及代谢中的功能提供了重要思路。
[Abstract]:Copper is an essential metal element in animal body and is involved in important metabolic reactions. The purpose of this study was to study the effects of copper free diet on the composition and related metabolic pathways of intestinal microbial colonies in mice. Twenty adult male rats with similar body weight and genetic background of wild type C57BL/6J were selected and randomly divided into 2 groups. There were 10 rats in the test group and 10 in the control group. The mice were fed with copper-free feed for 1 week, the control group was fed with copper-containing water for 25 mg/L, and the experimental group was fed with copper-free water for 30 days. At the end of the trial period, the caudal vein blood samples were collected for the determination of ceruloplasmin content, and the fresh caecum contents of mice were collected. The 16s rRNA genomes of the caecum contents were analyzed by high-throughput sequencing technique. The results showed that compared with the control group, the serum ceruloplasmin content in the copper free group was significantly lower than that in the control group (P0.001). There were 5 and 43 significant differences in the cecum microflora between the copper free group and the control group (P0.05). It was found that there were 19 KEGG secondary metabolic pathways in the two groups (P0.05), among which lipid metabolism, glycine biosynthesis and metabolism were related to metabolism. Metabolism of polyketones, terpenoids, other secondary metabolites, biosynthesis and amino acid metabolism suggests that the absence of copper in feed can change the composition and function of a few intestinal flora. This experiment provides an important way to study the function of copper in intestinal microflora structure and metabolism of mice.
【作者单位】： 中国农业科学院北京畜牧兽医研究所;
【基金】：中国农业科学院基本科研业务费预算增量项目(2015ywf-zd-30) 中国农业科学院北京畜牧兽医研究所科技创新工程特设项目(ASTIP-IAS-TS-02)
【分类号】：S816
本文编号：2389485
[Abstract]:Copper is an essential metal element in animal body and is involved in important metabolic reactions. The purpose of this study was to study the effects of copper free diet on the composition and related metabolic pathways of intestinal microbial colonies in mice. Twenty adult male rats with similar body weight and genetic background of wild type C57BL/6J were selected and randomly divided into 2 groups. There were 10 rats in the test group and 10 in the control group. The mice were fed with copper-free feed for 1 week, the control group was fed with copper-containing water for 25 mg/L, and the experimental group was fed with copper-free water for 30 days. At the end of the trial period, the caudal vein blood samples were collected for the determination of ceruloplasmin content, and the fresh caecum contents of mice were collected. The 16s rRNA genomes of the caecum contents were analyzed by high-throughput sequencing technique. The results showed that compared with the control group, the serum ceruloplasmin content in the copper free group was significantly lower than that in the control group (P0.001). There were 5 and 43 significant differences in the cecum microflora between the copper free group and the control group (P0.05). It was found that there were 19 KEGG secondary metabolic pathways in the two groups (P0.05), among which lipid metabolism, glycine biosynthesis and metabolism were related to metabolism. Metabolism of polyketones, terpenoids, other secondary metabolites, biosynthesis and amino acid metabolism suggests that the absence of copper in feed can change the composition and function of a few intestinal flora. This experiment provides an important way to study the function of copper in intestinal microflora structure and metabolism of mice.
【作者单位】： 中国农业科学院北京畜牧兽医研究所;
【基金】：中国农业科学院基本科研业务费预算增量项目(2015ywf-zd-30) 中国农业科学院北京畜牧兽医研究所科技创新工程特设项目(ASTIP-IAS-TS-02)
【分类号】：S816
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，本文编号：2389485
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